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The effect of formulation variables on the characteristics of insulin-loaded poly(lactic-co-glycolic acid) microspheres prepared by a single phase oil in oil solvent evaporation method.
Colloids Surf B Biointerfaces. 2009 Nov 01; 74(1):340-9.CS

Abstract

Biodegradable polymeric microspheres are ideal vehicles for controlled delivery applications of drugs, peptides and proteins. Amongst them, poly(lactic-co-glycolic acid) (PLGA) has generated enormous interest due to their favorable properties and also has been approved by FDA for drug delivery. Insulin-loaded PLGA microparticles were prepared by our developed single phase oil in oil (o/o) emulsion solvent evaporation technique. Insulin, a model protein, was successfully loaded into microparticles by changing experimental variables such as polymer molecular weight, polymer concentration, surfactant concentration and stirring speed in order to optimize process variables on drug encapsulation efficiency, release rates, size and size distribution. A 2(4) full factorial design was employed to evaluate systematically the combined effect of variables on responses. Scanning electron microscope (SEM) confirmed spherical shapes, smooth surface morphology and microsphere structure without aggregation. FTIR and DSC results showed drug-polymer interaction. The encapsulation efficiency of insulin was mainly influenced by surfactant concentration. Moreover, polymer concentration and polymer molecular weight affected burst release of drug and size characteristics of microspheres, respectively. It was concluded that using PLGA with higher molecular weight, high surfactant and polymer concentrations led to a more appropriate encapsulation efficiency of insulin with low burst effect and desirable release pattern.

Authors+Show Affiliations

Pharmaceutical Technology Laboratory, Drug Applied Research Center, Tabriz University (Medical Sciences), Tabriz, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19717287

Citation

Hamishehkar, Hamed, et al. "The Effect of Formulation Variables On the Characteristics of Insulin-loaded Poly(lactic-co-glycolic Acid) Microspheres Prepared By a Single Phase Oil in Oil Solvent Evaporation Method." Colloids and Surfaces. B, Biointerfaces, vol. 74, no. 1, 2009, pp. 340-9.
Hamishehkar H, Emami J, Najafabadi AR, et al. The effect of formulation variables on the characteristics of insulin-loaded poly(lactic-co-glycolic acid) microspheres prepared by a single phase oil in oil solvent evaporation method. Colloids Surf B Biointerfaces. 2009;74(1):340-9.
Hamishehkar, H., Emami, J., Najafabadi, A. R., Gilani, K., Minaiyan, M., Mahdavi, H., & Nokhodchi, A. (2009). The effect of formulation variables on the characteristics of insulin-loaded poly(lactic-co-glycolic acid) microspheres prepared by a single phase oil in oil solvent evaporation method. Colloids and Surfaces. B, Biointerfaces, 74(1), 340-9. https://doi.org/10.1016/j.colsurfb.2009.08.003
Hamishehkar H, et al. The Effect of Formulation Variables On the Characteristics of Insulin-loaded Poly(lactic-co-glycolic Acid) Microspheres Prepared By a Single Phase Oil in Oil Solvent Evaporation Method. Colloids Surf B Biointerfaces. 2009 Nov 1;74(1):340-9. PubMed PMID: 19717287.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of formulation variables on the characteristics of insulin-loaded poly(lactic-co-glycolic acid) microspheres prepared by a single phase oil in oil solvent evaporation method. AU - Hamishehkar,Hamed, AU - Emami,Jaber, AU - Najafabadi,Abdolhossein Rouholamini, AU - Gilani,Kambiz, AU - Minaiyan,Mohsen, AU - Mahdavi,Hamid, AU - Nokhodchi,Ali, Y1 - 2009/08/12/ PY - 2009/04/15/received PY - 2009/05/18/revised PY - 2009/08/04/accepted PY - 2009/9/1/entrez PY - 2009/9/1/pubmed PY - 2009/12/16/medline SP - 340 EP - 9 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 74 IS - 1 N2 - Biodegradable polymeric microspheres are ideal vehicles for controlled delivery applications of drugs, peptides and proteins. Amongst them, poly(lactic-co-glycolic acid) (PLGA) has generated enormous interest due to their favorable properties and also has been approved by FDA for drug delivery. Insulin-loaded PLGA microparticles were prepared by our developed single phase oil in oil (o/o) emulsion solvent evaporation technique. Insulin, a model protein, was successfully loaded into microparticles by changing experimental variables such as polymer molecular weight, polymer concentration, surfactant concentration and stirring speed in order to optimize process variables on drug encapsulation efficiency, release rates, size and size distribution. A 2(4) full factorial design was employed to evaluate systematically the combined effect of variables on responses. Scanning electron microscope (SEM) confirmed spherical shapes, smooth surface morphology and microsphere structure without aggregation. FTIR and DSC results showed drug-polymer interaction. The encapsulation efficiency of insulin was mainly influenced by surfactant concentration. Moreover, polymer concentration and polymer molecular weight affected burst release of drug and size characteristics of microspheres, respectively. It was concluded that using PLGA with higher molecular weight, high surfactant and polymer concentrations led to a more appropriate encapsulation efficiency of insulin with low burst effect and desirable release pattern. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/19717287/The_effect_of_formulation_variables_on_the_characteristics_of_insulin_loaded_poly_lactic_co_glycolic_acid__microspheres_prepared_by_a_single_phase_oil_in_oil_solvent_evaporation_method_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(09)00362-2 DB - PRIME DP - Unbound Medicine ER -