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Characterization of highly pathogenic H5N1 avian influenza viruses isolated from poultry markets in central China.
Virus Res. 2009 Dec; 146(1-2):19-28.VR

Abstract

H5N1 highly pathogenic avian influenza (HPAI) viruses have seriously affected the Asian poultry industry since their recurrence in 2003. While surveillance in southern China has revealed that H5N1 viruses underwent extensive genetic reassortment to generate many different viral genotype viruses, little is known concerning the genotypes of H5N1 virus that circulated in central China in recent years. In this study, 16 H5N1 influenza viruses were isolated from the poultry market in central China during late 2006 and early 2007, and the genotypes and pathogenicity of the viruses were identified and characterized. All eight segments of each virus were sequenced, and phylogenetic analysis showed that the two surface glycoprotein genes, hemagglutinin (HA) and neuraminidase (NA), of all the viruses were closely related to the H5N1 viruses isolated in poultry in southern China since 2006. Phylogenetic analysis of the internal protein genes indicated that four viral genotypes circulated in poultry markets in central China. The virulence of 7 of the 16 isolates was tested in chickens and mice. The results showed that the 7 isolates were highly pathogenic for SPF chickens, and had a varied virulence in mice. Our results indicate that the H5N1 viruses circulated in central China have diversified characteristics of genotype and virulence.

Authors+Show Affiliations

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Hubei, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19720095

Citation

Chen, Jianjun, et al. "Characterization of Highly Pathogenic H5N1 Avian Influenza Viruses Isolated From Poultry Markets in Central China." Virus Research, vol. 146, no. 1-2, 2009, pp. 19-28.
Chen J, Fang F, Yang Z, et al. Characterization of highly pathogenic H5N1 avian influenza viruses isolated from poultry markets in central China. Virus Res. 2009;146(1-2):19-28.
Chen, J., Fang, F., Yang, Z., Liu, X., Zhang, H., Zhang, Z., Zhang, X., & Chen, Z. (2009). Characterization of highly pathogenic H5N1 avian influenza viruses isolated from poultry markets in central China. Virus Research, 146(1-2), 19-28. https://doi.org/10.1016/j.virusres.2009.08.010
Chen J, et al. Characterization of Highly Pathogenic H5N1 Avian Influenza Viruses Isolated From Poultry Markets in Central China. Virus Res. 2009;146(1-2):19-28. PubMed PMID: 19720095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of highly pathogenic H5N1 avian influenza viruses isolated from poultry markets in central China. AU - Chen,Jianjun, AU - Fang,Fang, AU - Yang,Zhongdong, AU - Liu,Xueying, AU - Zhang,Hongbo, AU - Zhang,Zhiping, AU - Zhang,Xianen, AU - Chen,Ze, Y1 - 2009/08/29/ PY - 2009/04/21/received PY - 2009/08/13/revised PY - 2009/08/21/accepted PY - 2009/9/2/entrez PY - 2009/9/2/pubmed PY - 2010/2/27/medline SP - 19 EP - 28 JF - Virus research JO - Virus Res. VL - 146 IS - 1-2 N2 - H5N1 highly pathogenic avian influenza (HPAI) viruses have seriously affected the Asian poultry industry since their recurrence in 2003. While surveillance in southern China has revealed that H5N1 viruses underwent extensive genetic reassortment to generate many different viral genotype viruses, little is known concerning the genotypes of H5N1 virus that circulated in central China in recent years. In this study, 16 H5N1 influenza viruses were isolated from the poultry market in central China during late 2006 and early 2007, and the genotypes and pathogenicity of the viruses were identified and characterized. All eight segments of each virus were sequenced, and phylogenetic analysis showed that the two surface glycoprotein genes, hemagglutinin (HA) and neuraminidase (NA), of all the viruses were closely related to the H5N1 viruses isolated in poultry in southern China since 2006. Phylogenetic analysis of the internal protein genes indicated that four viral genotypes circulated in poultry markets in central China. The virulence of 7 of the 16 isolates was tested in chickens and mice. The results showed that the 7 isolates were highly pathogenic for SPF chickens, and had a varied virulence in mice. Our results indicate that the H5N1 viruses circulated in central China have diversified characteristics of genotype and virulence. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/19720095/Characterization_of_highly_pathogenic_H5N1_avian_influenza_viruses_isolated_from_poultry_markets_in_central_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(09)00294-9 DB - PRIME DP - Unbound Medicine ER -