Tags

Type your tag names separated by a space and hit enter

Solid-phase extraction of melamine from aqueous samples using water-compatible molecularly imprinted polymers.
J Sep Sci. 2009 Oct; 32(19):3310-8.JS

Abstract

A method for molecularly imprinted SPE of the melamine from environmental water samples was investigated. Cyromazine-imprinted polymers were synthesized in water-methanol systems for the selective extraction of melamine from aqueous samples, followed by HPLC analysis. Molecular recognition properties and binding capability to melamine were evaluated by adsorption test and Scatchard analysis, which showed the dissociation constant (KD) and the maximum binding quantity (Qmax) were 0.032 micromol/L and 4.77 micromol/g for high affinity binding site, and 0.26 micromol/L and 19.10 micromol/g for lower affinity binding site, respectively. Under the optimum extraction protocol, the method can be successfully applied to selectively extract and enrich melamine in environmental water. The linearity was ranged from 0.500 to 100.0 ng/mL (r > 0.999) in tap water, lake water, and seawater analysis. When 50 mL of the water samples loaded, the LODs of the method were ca. 0.1 ng/mL, and the LOQs were ca. 0.5 ng/mL. The mean recoveries of melamine from blank water samples spiked at 0.5, 5.0, and 50 ng/mL were more than 86.3%, with the RSD less than 8.8%.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, College of Veterinary Medicine, South China Agriculture University, Guangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19722175

Citation

He, Limin, et al. "Solid-phase Extraction of Melamine From Aqueous Samples Using Water-compatible Molecularly Imprinted Polymers." Journal of Separation Science, vol. 32, no. 19, 2009, pp. 3310-8.
He L, Su Y, Shen X, et al. Solid-phase extraction of melamine from aqueous samples using water-compatible molecularly imprinted polymers. J Sep Sci. 2009;32(19):3310-8.
He, L., Su, Y., Shen, X., Zheng, Y., Guo, H., & Zeng, Z. (2009). Solid-phase extraction of melamine from aqueous samples using water-compatible molecularly imprinted polymers. Journal of Separation Science, 32(19), 3310-8. https://doi.org/10.1002/jssc.200900407
He L, et al. Solid-phase Extraction of Melamine From Aqueous Samples Using Water-compatible Molecularly Imprinted Polymers. J Sep Sci. 2009;32(19):3310-8. PubMed PMID: 19722175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solid-phase extraction of melamine from aqueous samples using water-compatible molecularly imprinted polymers. AU - He,Limin, AU - Su,Yijuan, AU - Shen,Xiangguang, AU - Zheng,Yaqiu, AU - Guo,Hongbin, AU - Zeng,Zhenling, PY - 2009/9/2/entrez PY - 2009/9/2/pubmed PY - 2009/12/16/medline SP - 3310 EP - 8 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 19 N2 - A method for molecularly imprinted SPE of the melamine from environmental water samples was investigated. Cyromazine-imprinted polymers were synthesized in water-methanol systems for the selective extraction of melamine from aqueous samples, followed by HPLC analysis. Molecular recognition properties and binding capability to melamine were evaluated by adsorption test and Scatchard analysis, which showed the dissociation constant (KD) and the maximum binding quantity (Qmax) were 0.032 micromol/L and 4.77 micromol/g for high affinity binding site, and 0.26 micromol/L and 19.10 micromol/g for lower affinity binding site, respectively. Under the optimum extraction protocol, the method can be successfully applied to selectively extract and enrich melamine in environmental water. The linearity was ranged from 0.500 to 100.0 ng/mL (r > 0.999) in tap water, lake water, and seawater analysis. When 50 mL of the water samples loaded, the LODs of the method were ca. 0.1 ng/mL, and the LOQs were ca. 0.5 ng/mL. The mean recoveries of melamine from blank water samples spiked at 0.5, 5.0, and 50 ng/mL were more than 86.3%, with the RSD less than 8.8%. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/19722175/Solid_phase_extraction_of_melamine_from_aqueous_samples_using_water_compatible_molecularly_imprinted_polymers_ L2 - https://doi.org/10.1002/jssc.200900407 DB - PRIME DP - Unbound Medicine ER -