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Molecularly imprinted solid-phase extraction of cephalexin from water-based matrices.
J Sep Sci. 2009 Oct; 32(19):3319-26.JS

Abstract

In the present paper, we describe the synthesis of a cephalexin (CFX) molecularly imprinted polymer (MIP), the direct application of the MIP to SPE for the determination of CFX (which is a beta-lactam antibiotic) in human urine and the use of the MIP in a tandem SPE system to determine CFX in river water. The molecularly imprinted polymers (MIP) showed cross-selectivity for amoxicillin (AMX; also a beta-lactam antibiotic). This allowed both CFX and AMX to be quantified in acidified human urine, with recoveries of 78 and 60% for CFX and AMX, respectively, when the urine samples were spiked with CFX and AMX at 4 mg/L. These analyses were facile because the molecularly imprinted solid-phase extraction (MISPE) extracts were clear compared to the nonpurified samples. In order to increase the sample volume for river water analyses, a tandem SPE system incorporating a commercially available sorbent was implemented. With this set-up, CFX was determined with recoveries in excess of 50% when 200 mL of acidified river water samples spiked at 10 microg/L with CFX were percolated through the tandem system.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Beltran A
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain.
Fontanals N
No affiliation info available
Marcé RM
No affiliation info available
Cormack PA
No affiliation info available
Borrull F
No affiliation info available

MeSH

AmoxicillinAnti-Bacterial AgentsCephalexinChromatography, High Pressure LiquidHumansMolecular ImprintingMolecular StructurePenicillin VPolymersRiversSolid Phase ExtractionUrineWaterWater Pollutants, Chemical

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19722176

Citation

Beltran, Antoni, et al. "Molecularly Imprinted Solid-phase Extraction of Cephalexin From Water-based Matrices." Journal of Separation Science, vol. 32, no. 19, 2009, pp. 3319-26.
Beltran A, Fontanals N, Marcé RM, et al. Molecularly imprinted solid-phase extraction of cephalexin from water-based matrices. J Sep Sci. 2009;32(19):3319-26.
Beltran, A., Fontanals, N., Marcé, R. M., Cormack, P. A., & Borrull, F. (2009). Molecularly imprinted solid-phase extraction of cephalexin from water-based matrices. Journal of Separation Science, 32(19), 3319-26. https://doi.org/10.1002/jssc.200900268
Beltran A, et al. Molecularly Imprinted Solid-phase Extraction of Cephalexin From Water-based Matrices. J Sep Sci. 2009;32(19):3319-26. PubMed PMID: 19722176.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecularly imprinted solid-phase extraction of cephalexin from water-based matrices. AU - Beltran,Antoni, AU - Fontanals,Núria, AU - Marcé,Rosa M, AU - Cormack,Peter A G, AU - Borrull,Francesc, PY - 2009/9/2/entrez PY - 2009/9/2/pubmed PY - 2009/12/16/medline SP - 3319 EP - 26 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 19 N2 - In the present paper, we describe the synthesis of a cephalexin (CFX) molecularly imprinted polymer (MIP), the direct application of the MIP to SPE for the determination of CFX (which is a beta-lactam antibiotic) in human urine and the use of the MIP in a tandem SPE system to determine CFX in river water. The molecularly imprinted polymers (MIP) showed cross-selectivity for amoxicillin (AMX; also a beta-lactam antibiotic). This allowed both CFX and AMX to be quantified in acidified human urine, with recoveries of 78 and 60% for CFX and AMX, respectively, when the urine samples were spiked with CFX and AMX at 4 mg/L. These analyses were facile because the molecularly imprinted solid-phase extraction (MISPE) extracts were clear compared to the nonpurified samples. In order to increase the sample volume for river water analyses, a tandem SPE system incorporating a commercially available sorbent was implemented. With this set-up, CFX was determined with recoveries in excess of 50% when 200 mL of acidified river water samples spiked at 10 microg/L with CFX were percolated through the tandem system. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/19722176/Molecularly_imprinted_solid_phase_extraction_of_cephalexin_from_water_based_matrices_ L2 - https://doi.org/10.1002/jssc.200900268 DB - PRIME DP - Unbound Medicine ER -