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Functional characterization of two 13-lipoxygenase genes from olive fruit in relation to the biosynthesis of volatile compounds of virgin olive oil.
J Agric Food Chem. 2009 Oct 14; 57(19):9097-107.JA

Abstract

Two LOX cDNA clones, Oep1LOX2 and Oep2LOX2, have been isolated from olive (Olea europaea cv. Picual). Both deduced amino acid sequences showed significant similarity to known plant LOX2, and they contain an N-terminal chloroplastic transit peptide. Genomic Southern blot analyses suggest that at least three copies of Oep1LOX2 and one copy of Oep2LOX2 should be present in the olive genome. Linolenic acid proved to be the preferred substrate for both olive recombinant LOXs, and analyses of reaction products revealed that both enzymes produce primarily 13-hydroperoxides from linoleic and linolenic acids. Expression levels of both genes were measured in the mesocarp and seeds during development and ripening of Picual and Arbequina olive fruit along with the level of volatile compounds in the corresponding virgin olive oils. Biochemical and gene expression data suggest a major involvement of the Oep2LOX2 gene in the biosynthesis of virgin olive oil aroma compounds.

Authors+Show Affiliations

Department of Physiology and Technology of Plant Products, Instituto de la Grasa (CSIC), Seville, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19722522

Citation

Padilla, María N., et al. "Functional Characterization of Two 13-lipoxygenase Genes From Olive Fruit in Relation to the Biosynthesis of Volatile Compounds of Virgin Olive Oil." Journal of Agricultural and Food Chemistry, vol. 57, no. 19, 2009, pp. 9097-107.
Padilla MN, Hernández ML, Sanz C, et al. Functional characterization of two 13-lipoxygenase genes from olive fruit in relation to the biosynthesis of volatile compounds of virgin olive oil. J Agric Food Chem. 2009;57(19):9097-107.
Padilla, M. N., Hernández, M. L., Sanz, C., & Martínez-Rivas, J. M. (2009). Functional characterization of two 13-lipoxygenase genes from olive fruit in relation to the biosynthesis of volatile compounds of virgin olive oil. Journal of Agricultural and Food Chemistry, 57(19), 9097-107. https://doi.org/10.1021/jf901777j
Padilla MN, et al. Functional Characterization of Two 13-lipoxygenase Genes From Olive Fruit in Relation to the Biosynthesis of Volatile Compounds of Virgin Olive Oil. J Agric Food Chem. 2009 Oct 14;57(19):9097-107. PubMed PMID: 19722522.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional characterization of two 13-lipoxygenase genes from olive fruit in relation to the biosynthesis of volatile compounds of virgin olive oil. AU - Padilla,María N, AU - Hernández,M Luisa, AU - Sanz,Carlos, AU - Martínez-Rivas,José M, PY - 2009/9/3/entrez PY - 2009/9/3/pubmed PY - 2010/1/29/medline SP - 9097 EP - 107 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 19 N2 - Two LOX cDNA clones, Oep1LOX2 and Oep2LOX2, have been isolated from olive (Olea europaea cv. Picual). Both deduced amino acid sequences showed significant similarity to known plant LOX2, and they contain an N-terminal chloroplastic transit peptide. Genomic Southern blot analyses suggest that at least three copies of Oep1LOX2 and one copy of Oep2LOX2 should be present in the olive genome. Linolenic acid proved to be the preferred substrate for both olive recombinant LOXs, and analyses of reaction products revealed that both enzymes produce primarily 13-hydroperoxides from linoleic and linolenic acids. Expression levels of both genes were measured in the mesocarp and seeds during development and ripening of Picual and Arbequina olive fruit along with the level of volatile compounds in the corresponding virgin olive oils. Biochemical and gene expression data suggest a major involvement of the Oep2LOX2 gene in the biosynthesis of virgin olive oil aroma compounds. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19722522/Functional_characterization_of_two_13_lipoxygenase_genes_from_olive_fruit_in_relation_to_the_biosynthesis_of_volatile_compounds_of_virgin_olive_oil_ L2 - https://doi.org/10.1021/jf901777j DB - PRIME DP - Unbound Medicine ER -