Tags

Type your tag names separated by a space and hit enter

MUC5AC expression is increased in bronchial submucosal glands of stable COPD patients.
Histopathology. 2009 Sep; 55(3):321-31.H

Abstract

AIMS

Mucus expectoration is a common feature of chronic obstructive pulmonary disease (COPD). MUC5AC and MUC5B, the major mucins, are released predominantly from submucosal glands in the central airways. The aim was to investigate gland size and MUC5AC and MUC5B expression in bronchial rings from smokers with COPD compared with control groups.

METHODS AND RESULTS

Bronchial rings from 10 non-smoking subjects, 20 smokers with normal lung function and 20 smokers with COPD were studied. Periodic acid-Schiff (PAS) and Alcian blue histochemistry and MUC5AC and MUC5B immunohistochemistry followed by quantification of the immunoreactive area was performed. The area occupied by MUC5AC+ cells in bronchial submucosal glands was increased in COPD [20% (5.5-31.7%) gland area] compared with smokers with normal lung function [9.5% (2.5-17.5%); P < 0.05] and non-smoking subjects [2% (0.4-6.2%); P < 0.05]. The area occupied by MUC5AC+ cells in the bronchial surface epithelium was also increased in smokers (with/without COPD) [73.5% (25-92%) epithelial area] compared with non-smoking subjects [15% (2.7-32%); P < 0.01]. Gland size, PAS, Alcian blue staining and MUC5B expression were not significantly different among groups. MUC5AC expression correlated with the degree of airflow obstruction. MUC5AC and MUC5B expression correlated with pack-years.

CONCLUSIONS

COPD is associated with increased MUC5AC expression in submucosal glands, indicating that MUC5AC may be involved in the pathophysiology of the disease.

Authors+Show Affiliations

Centro di Ricerca su Asma e BPCO, University of Ferrara, Via Savonarola 9, Ferrara 44121, Italy. gaetano.caramori@unife.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19723147

Citation

Caramori, Gaetano, et al. "MUC5AC Expression Is Increased in Bronchial Submucosal Glands of Stable COPD Patients." Histopathology, vol. 55, no. 3, 2009, pp. 321-31.
Caramori G, Casolari P, Di Gregorio C, et al. MUC5AC expression is increased in bronchial submucosal glands of stable COPD patients. Histopathology. 2009;55(3):321-31.
Caramori, G., Casolari, P., Di Gregorio, C., Saetta, M., Baraldo, S., Boschetto, P., Ito, K., Fabbri, L. M., Barnes, P. J., Adcock, I. M., Cavallesco, G., Chung, K. F., & Papi, A. (2009). MUC5AC expression is increased in bronchial submucosal glands of stable COPD patients. Histopathology, 55(3), 321-31. https://doi.org/10.1111/j.1365-2559.2009.03377.x
Caramori G, et al. MUC5AC Expression Is Increased in Bronchial Submucosal Glands of Stable COPD Patients. Histopathology. 2009;55(3):321-31. PubMed PMID: 19723147.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MUC5AC expression is increased in bronchial submucosal glands of stable COPD patients. AU - Caramori,Gaetano, AU - Casolari,Paolo, AU - Di Gregorio,Carmela, AU - Saetta,Marina, AU - Baraldo,Simonetta, AU - Boschetto,Piera, AU - Ito,Kazuhiro, AU - Fabbri,Leonardo M, AU - Barnes,Peter J, AU - Adcock,Ian M, AU - Cavallesco,Giorgio, AU - Chung,Kian F, AU - Papi,Alberto, PY - 2009/9/3/entrez PY - 2009/9/3/pubmed PY - 2009/11/6/medline SP - 321 EP - 31 JF - Histopathology JO - Histopathology VL - 55 IS - 3 N2 - AIMS: Mucus expectoration is a common feature of chronic obstructive pulmonary disease (COPD). MUC5AC and MUC5B, the major mucins, are released predominantly from submucosal glands in the central airways. The aim was to investigate gland size and MUC5AC and MUC5B expression in bronchial rings from smokers with COPD compared with control groups. METHODS AND RESULTS: Bronchial rings from 10 non-smoking subjects, 20 smokers with normal lung function and 20 smokers with COPD were studied. Periodic acid-Schiff (PAS) and Alcian blue histochemistry and MUC5AC and MUC5B immunohistochemistry followed by quantification of the immunoreactive area was performed. The area occupied by MUC5AC+ cells in bronchial submucosal glands was increased in COPD [20% (5.5-31.7%) gland area] compared with smokers with normal lung function [9.5% (2.5-17.5%); P < 0.05] and non-smoking subjects [2% (0.4-6.2%); P < 0.05]. The area occupied by MUC5AC+ cells in the bronchial surface epithelium was also increased in smokers (with/without COPD) [73.5% (25-92%) epithelial area] compared with non-smoking subjects [15% (2.7-32%); P < 0.01]. Gland size, PAS, Alcian blue staining and MUC5B expression were not significantly different among groups. MUC5AC expression correlated with the degree of airflow obstruction. MUC5AC and MUC5B expression correlated with pack-years. CONCLUSIONS: COPD is associated with increased MUC5AC expression in submucosal glands, indicating that MUC5AC may be involved in the pathophysiology of the disease. SN - 1365-2559 UR - https://www.unboundmedicine.com/medline/citation/19723147/MUC5AC_expression_is_increased_in_bronchial_submucosal_glands_of_stable_COPD_patients_ L2 - https://doi.org/10.1111/j.1365-2559.2009.03377.x DB - PRIME DP - Unbound Medicine ER -