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Leptin affects intestinal epithelial cell turnover in correlation with leptin receptor expression along the villus-crypt axis after massive small bowel resection in a rat.
Pediatr Res. 2009 Dec; 66(6):648-53.PR

Abstract

In this study, we examine the responsiveness of intestinal epithelial cell turnover to leptin (LEP) in correlation with leptin receptor (LEPr) expression along the villus-crypt axis in a rat with short bowel syndrome (SBS). Adult rats underwent either a 75% intestinal resection or a transection. SBS-LEP rats underwent bowel resection and were treated with LEP starting from the fourth postoperative day. Parameters of intestinal adaptation, enterocyte proliferation, and enterocyte apoptosis were determined at sacrifice. RT-PCR technique was used to determine Bax and Bcl-2 gene expression in ileal mucosa. Villus tips, lateral villi, and crypts were separated using laser capture microdissection. LEPr expression for each compartment was assessed by quantitative real-time PCR (Taqman). Treatment with LEP significantly stimulated all parameters of adaptation. LEPr expression in crypts significantly increased in SBS rats (vs Sham rats) and was accompanied by a significant increase in enterocyte proliferation and decreased apoptosis after LEP administration. A significant increase in LEPr expression at the tip of the villus in SBS rats was accompanied by decreased cell apoptosis. In conclusion LEP accelerated enterocyte turnover and stimulated intestinal adaptation. The effect of LEP on enterocyte proliferation and enterocyte apoptosis correlated with receptor expression along the villus-crypt axis.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Sukhotnik I
Department Pediatric Surgery, Bnai Zion Medical Center, 47 Golomb St., P.O.B. 4940, Haifa 31048, Israel. igor-dr@internet-zahav.net
Coran AG
No affiliation info available
Mogilner JG
No affiliation info available
Shamian B
No affiliation info available
Karry R
No affiliation info available
Lieber M
No affiliation info available
Shaoul R
No affiliation info available

MeSH

Analysis of VarianceAnimalsApoptosisDNA PrimersEnterocytesIntestinal MucosaIntestine, SmallLeptinMaleMicrodissectionRatsRats, Sprague-DawleyReceptors, LeptinReverse Transcriptase Polymerase Chain Reaction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19730157

Citation

Sukhotnik, Igor, et al. "Leptin Affects Intestinal Epithelial Cell Turnover in Correlation With Leptin Receptor Expression Along the Villus-crypt Axis After Massive Small Bowel Resection in a Rat." Pediatric Research, vol. 66, no. 6, 2009, pp. 648-53.
Sukhotnik I, Coran AG, Mogilner JG, et al. Leptin affects intestinal epithelial cell turnover in correlation with leptin receptor expression along the villus-crypt axis after massive small bowel resection in a rat. Pediatr Res. 2009;66(6):648-53.
Sukhotnik, I., Coran, A. G., Mogilner, J. G., Shamian, B., Karry, R., Lieber, M., & Shaoul, R. (2009). Leptin affects intestinal epithelial cell turnover in correlation with leptin receptor expression along the villus-crypt axis after massive small bowel resection in a rat. Pediatric Research, 66(6), 648-53. https://doi.org/10.1203/PDR.0b013e3181be9f84
Sukhotnik I, et al. Leptin Affects Intestinal Epithelial Cell Turnover in Correlation With Leptin Receptor Expression Along the Villus-crypt Axis After Massive Small Bowel Resection in a Rat. Pediatr Res. 2009;66(6):648-53. PubMed PMID: 19730157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leptin affects intestinal epithelial cell turnover in correlation with leptin receptor expression along the villus-crypt axis after massive small bowel resection in a rat. AU - Sukhotnik,Igor, AU - Coran,Arnold G, AU - Mogilner,Jorge G, AU - Shamian,Benhoor, AU - Karry,Rahel, AU - Lieber,Michael, AU - Shaoul,Ron, PY - 2009/9/5/entrez PY - 2009/9/5/pubmed PY - 2010/3/23/medline SP - 648 EP - 53 JF - Pediatric research JO - Pediatr Res VL - 66 IS - 6 N2 - In this study, we examine the responsiveness of intestinal epithelial cell turnover to leptin (LEP) in correlation with leptin receptor (LEPr) expression along the villus-crypt axis in a rat with short bowel syndrome (SBS). Adult rats underwent either a 75% intestinal resection or a transection. SBS-LEP rats underwent bowel resection and were treated with LEP starting from the fourth postoperative day. Parameters of intestinal adaptation, enterocyte proliferation, and enterocyte apoptosis were determined at sacrifice. RT-PCR technique was used to determine Bax and Bcl-2 gene expression in ileal mucosa. Villus tips, lateral villi, and crypts were separated using laser capture microdissection. LEPr expression for each compartment was assessed by quantitative real-time PCR (Taqman). Treatment with LEP significantly stimulated all parameters of adaptation. LEPr expression in crypts significantly increased in SBS rats (vs Sham rats) and was accompanied by a significant increase in enterocyte proliferation and decreased apoptosis after LEP administration. A significant increase in LEPr expression at the tip of the villus in SBS rats was accompanied by decreased cell apoptosis. In conclusion LEP accelerated enterocyte turnover and stimulated intestinal adaptation. The effect of LEP on enterocyte proliferation and enterocyte apoptosis correlated with receptor expression along the villus-crypt axis. SN - 1530-0447 UR - https://www.unboundmedicine.com/medline/citation/19730157/Leptin_affects_intestinal_epithelial_cell_turnover_in_correlation_with_leptin_receptor_expression_along_the_villus_crypt_axis_after_massive_small_bowel_resection_in_a_rat_ DB - PRIME DP - Unbound Medicine ER -