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Neuroprotective effect of baicalein on hydrogen peroxide-mediated oxidative stress and mitochondrial dysfunction in PC12 cells.
J Mol Neurosci. 2010 Mar; 40(3):311-20.JM

Abstract

Oxidative stress plays an important role in many neurodegenerative disorders. In this study, the effect of baicalein, a natural flavonoid isolated from the root of Scutellaria baicalensis G., on hydrogen peroxide (H2O2)-induced cytotoxicity in PC12 cells were investigated. Exposure of PC12 cells to 0.15 mM H2O2 for 20 min induced a significant decrease in cell viability accompanied by increased oxidative stress, mitochondrial dysfunction, downregulation of Bcl-2, upregulation of Bax, and cell apoptosis. Pretreatment of PC12 cells with baicalein inhibited H2O2-induced cell viability loss, intracellular reactive oxygen species generation, and lipid peroxidation in a dose-dependent manner. Meanwhile, baicalein potentially inhibited H2O2-induced cell apoptosis characterized with the DNA fragment. And the mitochondrial pathway involving the mitochondrial dysfunction associated with cell apoptosis including membrane potential loss, the release of cytochrome c, the downregulation of Bcl-2, upregulation of Bax induced by H2O2 were also abrogated in the presence of baicalein. Taken together, these results suggest that baicalein can block H2O2-induced apoptosis by prevention of oxidative stress as well as regulation of Bcl-2 family members and suppression of mitochondria dysfunction, which might be beneficial for the treatment of oxidative stress in aging and age-associated neurodegenerative diseases.

Authors+Show Affiliations

Laboratory of Fiber Materials and Modern Textile Growing Base for State Key Laboratory, Qingdao University, 308 Ningxia Road, Qingdao, 266071, People's Republic of China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19731100

Citation

Zhang, Shehua, et al. "Neuroprotective Effect of Baicalein On Hydrogen Peroxide-mediated Oxidative Stress and Mitochondrial Dysfunction in PC12 Cells." Journal of Molecular Neuroscience : MN, vol. 40, no. 3, 2010, pp. 311-20.
Zhang S, Ye J, Dong G. Neuroprotective effect of baicalein on hydrogen peroxide-mediated oxidative stress and mitochondrial dysfunction in PC12 cells. J Mol Neurosci. 2010;40(3):311-20.
Zhang, S., Ye, J., & Dong, G. (2010). Neuroprotective effect of baicalein on hydrogen peroxide-mediated oxidative stress and mitochondrial dysfunction in PC12 cells. Journal of Molecular Neuroscience : MN, 40(3), 311-20. https://doi.org/10.1007/s12031-009-9285-5
Zhang S, Ye J, Dong G. Neuroprotective Effect of Baicalein On Hydrogen Peroxide-mediated Oxidative Stress and Mitochondrial Dysfunction in PC12 Cells. J Mol Neurosci. 2010;40(3):311-20. PubMed PMID: 19731100.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effect of baicalein on hydrogen peroxide-mediated oxidative stress and mitochondrial dysfunction in PC12 cells. AU - Zhang,Shehua, AU - Ye,Junli, AU - Dong,Guoxiong, PY - 2009/04/16/received PY - 2009/08/03/accepted PY - 2009/9/5/entrez PY - 2009/9/5/pubmed PY - 2010/5/8/medline SP - 311 EP - 20 JF - Journal of molecular neuroscience : MN JO - J Mol Neurosci VL - 40 IS - 3 N2 - Oxidative stress plays an important role in many neurodegenerative disorders. In this study, the effect of baicalein, a natural flavonoid isolated from the root of Scutellaria baicalensis G., on hydrogen peroxide (H2O2)-induced cytotoxicity in PC12 cells were investigated. Exposure of PC12 cells to 0.15 mM H2O2 for 20 min induced a significant decrease in cell viability accompanied by increased oxidative stress, mitochondrial dysfunction, downregulation of Bcl-2, upregulation of Bax, and cell apoptosis. Pretreatment of PC12 cells with baicalein inhibited H2O2-induced cell viability loss, intracellular reactive oxygen species generation, and lipid peroxidation in a dose-dependent manner. Meanwhile, baicalein potentially inhibited H2O2-induced cell apoptosis characterized with the DNA fragment. And the mitochondrial pathway involving the mitochondrial dysfunction associated with cell apoptosis including membrane potential loss, the release of cytochrome c, the downregulation of Bcl-2, upregulation of Bax induced by H2O2 were also abrogated in the presence of baicalein. Taken together, these results suggest that baicalein can block H2O2-induced apoptosis by prevention of oxidative stress as well as regulation of Bcl-2 family members and suppression of mitochondria dysfunction, which might be beneficial for the treatment of oxidative stress in aging and age-associated neurodegenerative diseases. SN - 1559-1166 UR - https://www.unboundmedicine.com/medline/citation/19731100/Neuroprotective_effect_of_baicalein_on_hydrogen_peroxide_mediated_oxidative_stress_and_mitochondrial_dysfunction_in_PC12_cells_ L2 - https://dx.doi.org/10.1007/s12031-009-9285-5 DB - PRIME DP - Unbound Medicine ER -