Citation
Saito, Yoshiro, et al. "Preparation and Application of Monoclonal Antibodies Against Oxidized DJ-1. Significant Elevation of Oxidized DJ-1 in Erythrocytes of Early-stage Parkinson Disease Patients." Neuroscience Letters, vol. 465, no. 1, 2009, pp. 1-5.
Saito Y, Hamakubo T, Yoshida Y, et al. Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients. Neurosci Lett. 2009;465(1):1-5.
Saito, Y., Hamakubo, T., Yoshida, Y., Ogawa, Y., Hara, Y., Fujimura, H., Imai, Y., Iwanari, H., Mochizuki, Y., Shichiri, M., Nishio, K., Kinumi, T., Noguchi, N., Kodama, T., & Niki, E. (2009). Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients. Neuroscience Letters, 465(1), 1-5. https://doi.org/10.1016/j.neulet.2009.08.074
Saito Y, et al. Preparation and Application of Monoclonal Antibodies Against Oxidized DJ-1. Significant Elevation of Oxidized DJ-1 in Erythrocytes of Early-stage Parkinson Disease Patients. Neurosci Lett. 2009 Nov 6;465(1):1-5. PubMed PMID: 19733211.
TY - JOUR
T1 - Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients.
AU - Saito,Yoshiro,
AU - Hamakubo,Takao,
AU - Yoshida,Yasukazu,
AU - Ogawa,Yoko,
AU - Hara,Yasuo,
AU - Fujimura,Harutoshi,
AU - Imai,Yasuharu,
AU - Iwanari,Hiroko,
AU - Mochizuki,Yasuhiro,
AU - Shichiri,Mototada,
AU - Nishio,Keiko,
AU - Kinumi,Tomoya,
AU - Noguchi,Noriko,
AU - Kodama,Tatsuhiko,
AU - Niki,Etsuo,
Y1 - 2009/09/03/
PY - 2009/05/11/received
PY - 2009/08/25/revised
PY - 2009/08/29/accepted
PY - 2009/9/8/entrez
PY - 2009/9/8/pubmed
PY - 2010/1/15/medline
SP - 1
EP - 5
JF - Neuroscience letters
JO - Neurosci Lett
VL - 465
IS - 1
N2 - DJ-1 was initially identified as a novel oncogene and has recently been found to be a causative gene for a familial form of Parkinson's disease (PD), viz, PARK7. Cysteine residue at position 106 (Cys-106) in DJ-1 was found to be oxidized preferentially under oxidative stress. In the present study, we developed specific antibodies against Cys-106-oxidized DJ-1 using baculovirus particles displaying the surface glycoprotein gp64-fusion protein as the immunizing agent. Western blot analysis combined with two-dimensional gel electrophoresis revealed that these antibodies specifically recognized oxidized DJ-1. Furthermore, we developed a competitive enzyme-linked immunosorbent assay (ELISA) for detecting oxidized DJ-1 and measured blood levels of oxidized DJ-1 in PD patients (n=15). It was observed that the levels of oxidized DJ-1 in erythrocytes of unmedicated PD patients were markedly higher without overlap than those of medicated PD patients and healthy subjects. No significant difference was observed in DJ-1 levels between mediated and unmediated PD patient. These results suggest the oxidative modification of DJ-1 in PD patients and the potential application of the antibody for diagnosis of PD at early-stage.
SN - 1872-7972
UR - https://www.unboundmedicine.com/medline/citation/19733211/Preparation_and_application_of_monoclonal_antibodies_against_oxidized_DJ_1__Significant_elevation_of_oxidized_DJ_1_in_erythrocytes_of_early_stage_Parkinson_disease_patients_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(09)01182-3
DB - PRIME
DP - Unbound Medicine
ER -
