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Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients.
Neurosci Lett. 2009 Nov 06; 465(1):1-5.NL

Abstract

DJ-1 was initially identified as a novel oncogene and has recently been found to be a causative gene for a familial form of Parkinson's disease (PD), viz, PARK7. Cysteine residue at position 106 (Cys-106) in DJ-1 was found to be oxidized preferentially under oxidative stress. In the present study, we developed specific antibodies against Cys-106-oxidized DJ-1 using baculovirus particles displaying the surface glycoprotein gp64-fusion protein as the immunizing agent. Western blot analysis combined with two-dimensional gel electrophoresis revealed that these antibodies specifically recognized oxidized DJ-1. Furthermore, we developed a competitive enzyme-linked immunosorbent assay (ELISA) for detecting oxidized DJ-1 and measured blood levels of oxidized DJ-1 in PD patients (n=15). It was observed that the levels of oxidized DJ-1 in erythrocytes of unmedicated PD patients were markedly higher without overlap than those of medicated PD patients and healthy subjects. No significant difference was observed in DJ-1 levels between mediated and unmediated PD patient. These results suggest the oxidative modification of DJ-1 in PD patients and the potential application of the antibody for diagnosis of PD at early-stage.

Authors+Show Affiliations

Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan. ysaito@mail.doshisha.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19733211

Citation

Saito, Yoshiro, et al. "Preparation and Application of Monoclonal Antibodies Against Oxidized DJ-1. Significant Elevation of Oxidized DJ-1 in Erythrocytes of Early-stage Parkinson Disease Patients." Neuroscience Letters, vol. 465, no. 1, 2009, pp. 1-5.
Saito Y, Hamakubo T, Yoshida Y, et al. Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients. Neurosci Lett. 2009;465(1):1-5.
Saito, Y., Hamakubo, T., Yoshida, Y., Ogawa, Y., Hara, Y., Fujimura, H., Imai, Y., Iwanari, H., Mochizuki, Y., Shichiri, M., Nishio, K., Kinumi, T., Noguchi, N., Kodama, T., & Niki, E. (2009). Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients. Neuroscience Letters, 465(1), 1-5. https://doi.org/10.1016/j.neulet.2009.08.074
Saito Y, et al. Preparation and Application of Monoclonal Antibodies Against Oxidized DJ-1. Significant Elevation of Oxidized DJ-1 in Erythrocytes of Early-stage Parkinson Disease Patients. Neurosci Lett. 2009 Nov 6;465(1):1-5. PubMed PMID: 19733211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients. AU - Saito,Yoshiro, AU - Hamakubo,Takao, AU - Yoshida,Yasukazu, AU - Ogawa,Yoko, AU - Hara,Yasuo, AU - Fujimura,Harutoshi, AU - Imai,Yasuharu, AU - Iwanari,Hiroko, AU - Mochizuki,Yasuhiro, AU - Shichiri,Mototada, AU - Nishio,Keiko, AU - Kinumi,Tomoya, AU - Noguchi,Noriko, AU - Kodama,Tatsuhiko, AU - Niki,Etsuo, Y1 - 2009/09/03/ PY - 2009/05/11/received PY - 2009/08/25/revised PY - 2009/08/29/accepted PY - 2009/9/8/entrez PY - 2009/9/8/pubmed PY - 2010/1/15/medline SP - 1 EP - 5 JF - Neuroscience letters JO - Neurosci Lett VL - 465 IS - 1 N2 - DJ-1 was initially identified as a novel oncogene and has recently been found to be a causative gene for a familial form of Parkinson's disease (PD), viz, PARK7. Cysteine residue at position 106 (Cys-106) in DJ-1 was found to be oxidized preferentially under oxidative stress. In the present study, we developed specific antibodies against Cys-106-oxidized DJ-1 using baculovirus particles displaying the surface glycoprotein gp64-fusion protein as the immunizing agent. Western blot analysis combined with two-dimensional gel electrophoresis revealed that these antibodies specifically recognized oxidized DJ-1. Furthermore, we developed a competitive enzyme-linked immunosorbent assay (ELISA) for detecting oxidized DJ-1 and measured blood levels of oxidized DJ-1 in PD patients (n=15). It was observed that the levels of oxidized DJ-1 in erythrocytes of unmedicated PD patients were markedly higher without overlap than those of medicated PD patients and healthy subjects. No significant difference was observed in DJ-1 levels between mediated and unmediated PD patient. These results suggest the oxidative modification of DJ-1 in PD patients and the potential application of the antibody for diagnosis of PD at early-stage. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/19733211/Preparation_and_application_of_monoclonal_antibodies_against_oxidized_DJ_1__Significant_elevation_of_oxidized_DJ_1_in_erythrocytes_of_early_stage_Parkinson_disease_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(09)01182-3 DB - PRIME DP - Unbound Medicine ER -