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Evaluation of dengue NS1 antigen detection tests with acute sera from patients infected with dengue virus in Venezuela.
Diagn Microbiol Infect Dis. 2009 Nov; 65(3):247-53.DM

Abstract

The performances of 2 commercial enzyme-linked immunosorbent assay (ELISA) kits (PLATELIA Dengue NS1 AG and Dengue Early ELISA) and a rapid immunochromatography test (Dengue NS1 AG Strip) for detection of dengue NS1 protein were compared using a panel of 87 sera from viremic dengue patients, as well as 36 sera from patients with other acute febrile illnesses. PLATELIA was more sensitive and slightly less specific than Dengue Early ELISA (sensitivity, 71.3% versus 60.9%; specificity, 86.1% versus 94.3%, respectively). The strip test showed an overall sensitivity of 67.8% with a specificity of 94.4%. A lower sensitivity was observed with Dengue Early ELISA for dengue virus (DENV) type 4 (30%) and by the 3 tests for DENV type 2 (56.5%). The use of these kits allows for rapid and specific early diagnosis of dengue infection; however, their sensitivity for each serotype must be further evaluated to guarantee an accurate diagnosis, particularly in those regions where the 4 dengue serotypes are cocirculating.

Authors+Show Affiliations

Instituto Venezolano de Investigaciones Científicas, Centro de Microbiología y Biología Celular, Laboratorio de Biología de Virus, Caracas 1020A, Venezuela. alramire@ivic.veNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19733994

Citation

Ramirez, Alvaro H., et al. "Evaluation of Dengue NS1 Antigen Detection Tests With Acute Sera From Patients Infected With Dengue Virus in Venezuela." Diagnostic Microbiology and Infectious Disease, vol. 65, no. 3, 2009, pp. 247-53.
Ramirez AH, Moros Z, Comach G, et al. Evaluation of dengue NS1 antigen detection tests with acute sera from patients infected with dengue virus in Venezuela. Diagn Microbiol Infect Dis. 2009;65(3):247-53.
Ramirez, A. H., Moros, Z., Comach, G., Zambrano, J., Bravo, L., Pinto, B., Vielma, S., Cardier, J., & Liprandi, F. (2009). Evaluation of dengue NS1 antigen detection tests with acute sera from patients infected with dengue virus in Venezuela. Diagnostic Microbiology and Infectious Disease, 65(3), 247-53. https://doi.org/10.1016/j.diagmicrobio.2009.07.022
Ramirez AH, et al. Evaluation of Dengue NS1 Antigen Detection Tests With Acute Sera From Patients Infected With Dengue Virus in Venezuela. Diagn Microbiol Infect Dis. 2009;65(3):247-53. PubMed PMID: 19733994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of dengue NS1 antigen detection tests with acute sera from patients infected with dengue virus in Venezuela. AU - Ramirez,Alvaro H, AU - Moros,Zoila, AU - Comach,Guillermo, AU - Zambrano,Julio, AU - Bravo,Lola, AU - Pinto,Belqys, AU - Vielma,Silvana, AU - Cardier,José, AU - Liprandi,Ferdinando, Y1 - 2009/09/05/ PY - 2009/04/01/received PY - 2009/07/20/revised PY - 2009/07/27/accepted PY - 2009/9/8/entrez PY - 2009/9/8/pubmed PY - 2010/2/2/medline SP - 247 EP - 53 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 65 IS - 3 N2 - The performances of 2 commercial enzyme-linked immunosorbent assay (ELISA) kits (PLATELIA Dengue NS1 AG and Dengue Early ELISA) and a rapid immunochromatography test (Dengue NS1 AG Strip) for detection of dengue NS1 protein were compared using a panel of 87 sera from viremic dengue patients, as well as 36 sera from patients with other acute febrile illnesses. PLATELIA was more sensitive and slightly less specific than Dengue Early ELISA (sensitivity, 71.3% versus 60.9%; specificity, 86.1% versus 94.3%, respectively). The strip test showed an overall sensitivity of 67.8% with a specificity of 94.4%. A lower sensitivity was observed with Dengue Early ELISA for dengue virus (DENV) type 4 (30%) and by the 3 tests for DENV type 2 (56.5%). The use of these kits allows for rapid and specific early diagnosis of dengue infection; however, their sensitivity for each serotype must be further evaluated to guarantee an accurate diagnosis, particularly in those regions where the 4 dengue serotypes are cocirculating. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/19733994/Evaluation_of_dengue_NS1_antigen_detection_tests_with_acute_sera_from_patients_infected_with_dengue_virus_in_Venezuela_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(09)00324-1 DB - PRIME DP - Unbound Medicine ER -