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The cyclization of the 3,6-anhydro-galactose ring of iota-carrageenan is catalyzed by two D-galactose-2,6-sulfurylases in the red alga Chondrus crispus.
Plant Physiol. 2009 Nov; 151(3):1609-16.PP

Abstract

Carrageenans are sulfated galactans found in the cell walls of numerous red seaweeds (Rhodophyta). They are classified according to the number and the position of sulfate ester groups and the occurrence of 3,6-anhydro-galactose. Although the carrageenan biosynthesis pathway is not fully understood, it is usually accepted that the last step consists of the formation of a 3,6-anhydro ring found in kappa- and iota-carrageenans through the enzymatic conversion of d-galactose-6-sulfate or d-galactose-2,6-disulfate occurring in mu- and nu-carrageenan, respectively. We purified two enzymes, sulfurylase I (65 kD) and sulfurylase II (32 kD), that are able to catalyze the conversion of nu- into iota-carrageenan. We compared their sulfate release rates (i.e. arising from the formation of the anhydro ring) with the viscosity of the solution and demonstrated two distinct modes of action. In addition, we found that some mixtures of sulfurylase I and II lead to the formation of carrageenan solutions with unexpectedly low viscosities. We discuss the implication of these findings for the assembly of a densely aggregated matrix in red algal cell walls.

Authors+Show Affiliations

Marine Plants and Biomolecules (UMR7139), Pierre and Marie Curie University-CNRS, Station Biologique, F-29682 Roscoff cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19734263

Citation

Genicot-Joncour, Sabine, et al. "The Cyclization of the 3,6-anhydro-galactose Ring of Iota-carrageenan Is Catalyzed By Two D-galactose-2,6-sulfurylases in the Red Alga Chondrus Crispus." Plant Physiology, vol. 151, no. 3, 2009, pp. 1609-16.
Genicot-Joncour S, Poinas A, Richard O, et al. The cyclization of the 3,6-anhydro-galactose ring of iota-carrageenan is catalyzed by two D-galactose-2,6-sulfurylases in the red alga Chondrus crispus. Plant Physiol. 2009;151(3):1609-16.
Genicot-Joncour, S., Poinas, A., Richard, O., Potin, P., Rudolph, B., Kloareg, B., & Helbert, W. (2009). The cyclization of the 3,6-anhydro-galactose ring of iota-carrageenan is catalyzed by two D-galactose-2,6-sulfurylases in the red alga Chondrus crispus. Plant Physiology, 151(3), 1609-16. https://doi.org/10.1104/pp.109.144329
Genicot-Joncour S, et al. The Cyclization of the 3,6-anhydro-galactose Ring of Iota-carrageenan Is Catalyzed By Two D-galactose-2,6-sulfurylases in the Red Alga Chondrus Crispus. Plant Physiol. 2009;151(3):1609-16. PubMed PMID: 19734263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The cyclization of the 3,6-anhydro-galactose ring of iota-carrageenan is catalyzed by two D-galactose-2,6-sulfurylases in the red alga Chondrus crispus. AU - Genicot-Joncour,Sabine, AU - Poinas,Alexandra, AU - Richard,Odile, AU - Potin,Philippe, AU - Rudolph,Brian, AU - Kloareg,Bernard, AU - Helbert,William, Y1 - 2009/09/04/ PY - 2009/9/8/entrez PY - 2009/9/8/pubmed PY - 2010/1/28/medline SP - 1609 EP - 16 JF - Plant physiology JO - Plant Physiol VL - 151 IS - 3 N2 - Carrageenans are sulfated galactans found in the cell walls of numerous red seaweeds (Rhodophyta). They are classified according to the number and the position of sulfate ester groups and the occurrence of 3,6-anhydro-galactose. Although the carrageenan biosynthesis pathway is not fully understood, it is usually accepted that the last step consists of the formation of a 3,6-anhydro ring found in kappa- and iota-carrageenans through the enzymatic conversion of d-galactose-6-sulfate or d-galactose-2,6-disulfate occurring in mu- and nu-carrageenan, respectively. We purified two enzymes, sulfurylase I (65 kD) and sulfurylase II (32 kD), that are able to catalyze the conversion of nu- into iota-carrageenan. We compared their sulfate release rates (i.e. arising from the formation of the anhydro ring) with the viscosity of the solution and demonstrated two distinct modes of action. In addition, we found that some mixtures of sulfurylase I and II lead to the formation of carrageenan solutions with unexpectedly low viscosities. We discuss the implication of these findings for the assembly of a densely aggregated matrix in red algal cell walls. SN - 1532-2548 UR - https://www.unboundmedicine.com/medline/citation/19734263/The_cyclization_of_the_36_anhydro_galactose_ring_of_iota_carrageenan_is_catalyzed_by_two_D_galactose_26_sulfurylases_in_the_red_alga_Chondrus_crispus_ L2 - https://academic.oup.com/plphys/article-lookup/doi/10.1104/pp.109.144329 DB - PRIME DP - Unbound Medicine ER -