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Preventive efficacy of hydroalcoholic extract of Cymbopogon citratus against radiation-induced DNA damage on V79 cells and free radical scavenging ability against radicals generated in vitro.
Hum Exp Toxicol. 2009 Apr; 28(4):195-202.HE

Abstract

This study presents the findings of free radical scavenging and antigenotoxic effect of hydroalcoholic extract of Cymbopogon citratus (CCE). The CCE at a concentration of 60 microg/mL resulted in a significant scavenging ability of 2,2-diphenyl-2-picryl hydrazyl (DPPH; (85%), 2,2-azinobis (3-ethyl benzothiazoline-6-sulphonic acid) (ABTS; 77%), hydroxyl (70%), superoxide (76%), nitric oxide (78%) free radicals generated using in vitro and also a moderate anti-lipid peroxidative effect (57%). Further, the radiation-induced antigenotoxic potential of CCE was assessed in Chinese hamster lung fibroblast cells (V79) using micronucleus assay. The CCE resulted in a dose-dependent decrease in the yield of radiation-induced micronuclei, with a maximum effect at 125 microg/mL CCE for 1 h before 2 Gy of radiation. Similarly, there was a significant (P < 0.05-0.0001) decrease in percentage of micronuclei when V79 cells were treated with optimal dose of CCE (125 microg/mL) before exposure to different doses of gamma radiation, that is, 0.5-4 Gy, compared with radiation alone groups. The results of the micronucleus study indicated antigenotoxic effect demonstrating the radioprotective potential of CCE and, which may partly due to its and antioxidant capacity as it presented its ability to scavenge various free radicals in vitro and anti-lipid peroxidative potential.

Authors+Show Affiliations

Division of Radiobiology & Toxicology, Manipal Life Sciences Center, Manipal University, Karnataka, India. satishraomlsc@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19734270

Citation

Rao, B S S., et al. "Preventive Efficacy of Hydroalcoholic Extract of Cymbopogon Citratus Against Radiation-induced DNA Damage On V79 Cells and Free Radical Scavenging Ability Against Radicals Generated in Vitro." Human & Experimental Toxicology, vol. 28, no. 4, 2009, pp. 195-202.
Rao BS, Shanbhoge R, Rao BN, et al. Preventive efficacy of hydroalcoholic extract of Cymbopogon citratus against radiation-induced DNA damage on V79 cells and free radical scavenging ability against radicals generated in vitro. Hum Exp Toxicol. 2009;28(4):195-202.
Rao, B. S., Shanbhoge, R., Rao, B. N., Adiga, S. K., Upadhya, D., Aithal, B. K., & Kumar, M. R. (2009). Preventive efficacy of hydroalcoholic extract of Cymbopogon citratus against radiation-induced DNA damage on V79 cells and free radical scavenging ability against radicals generated in vitro. Human & Experimental Toxicology, 28(4), 195-202. https://doi.org/10.1177/0960327109104822
Rao BS, et al. Preventive Efficacy of Hydroalcoholic Extract of Cymbopogon Citratus Against Radiation-induced DNA Damage On V79 Cells and Free Radical Scavenging Ability Against Radicals Generated in Vitro. Hum Exp Toxicol. 2009;28(4):195-202. PubMed PMID: 19734270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preventive efficacy of hydroalcoholic extract of Cymbopogon citratus against radiation-induced DNA damage on V79 cells and free radical scavenging ability against radicals generated in vitro. AU - Rao,B S S, AU - Shanbhoge,R, AU - Rao,B N, AU - Adiga,S K, AU - Upadhya,D, AU - Aithal,B K, AU - Kumar,M R S, PY - 2009/9/8/entrez PY - 2009/9/8/pubmed PY - 2009/12/16/medline SP - 195 EP - 202 JF - Human & experimental toxicology JO - Hum Exp Toxicol VL - 28 IS - 4 N2 - This study presents the findings of free radical scavenging and antigenotoxic effect of hydroalcoholic extract of Cymbopogon citratus (CCE). The CCE at a concentration of 60 microg/mL resulted in a significant scavenging ability of 2,2-diphenyl-2-picryl hydrazyl (DPPH; (85%), 2,2-azinobis (3-ethyl benzothiazoline-6-sulphonic acid) (ABTS; 77%), hydroxyl (70%), superoxide (76%), nitric oxide (78%) free radicals generated using in vitro and also a moderate anti-lipid peroxidative effect (57%). Further, the radiation-induced antigenotoxic potential of CCE was assessed in Chinese hamster lung fibroblast cells (V79) using micronucleus assay. The CCE resulted in a dose-dependent decrease in the yield of radiation-induced micronuclei, with a maximum effect at 125 microg/mL CCE for 1 h before 2 Gy of radiation. Similarly, there was a significant (P < 0.05-0.0001) decrease in percentage of micronuclei when V79 cells were treated with optimal dose of CCE (125 microg/mL) before exposure to different doses of gamma radiation, that is, 0.5-4 Gy, compared with radiation alone groups. The results of the micronucleus study indicated antigenotoxic effect demonstrating the radioprotective potential of CCE and, which may partly due to its and antioxidant capacity as it presented its ability to scavenge various free radicals in vitro and anti-lipid peroxidative potential. SN - 1477-0903 UR - https://www.unboundmedicine.com/medline/citation/19734270/Preventive_efficacy_of_hydroalcoholic_extract_of_Cymbopogon_citratus_against_radiation_induced_DNA_damage_on_V79_cells_and_free_radical_scavenging_ability_against_radicals_generated_in_vitro_ L2 - https://journals.sagepub.com/doi/10.1177/0960327109104822?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -