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Roles of TLR2, TLR4, NOD2, and NOD1 in pulp fibroblasts.
J Dent Res 2009; 88(8):762-7JD

Abstract

Pulp fibroblasts express various pro-inflammatory mediators leading to marked infiltration of inflammatory cells in the progression of pulpitis. We hypothesized that pulp fibroblasts play roles in the recognition of invaded caries-related bacteria and the subsequent innate immune responses. We found clear expressions of TLR2, NOD1, and NOD2 and a faint expression of TLR4 in human dental pulp fibroblasts (HDPF) by RT-PCR and flow cytometry. We also observed that various pro-inflammatory mediators, including cytokines, chemokines, adhesion molecules, prostaglandin E(2) and its key enzyme COX-2, not iNOS or caspase-1, were markedly up-regulated by stimulation with these TLR and NOD agonists. More over, the NOD2 agonist acted synergistically with the TLR2, not the TLR4, agonist to stimulate the production of pro-inflammatory mediators in HDPF. These findings indicate that TLR2, TLR4, NOD2, and NOD1 in HDPF are functional receptors, and NOD2 is a modulator of signals transmitted through TLR2 in pulpal immune responses, leading to progressive pulpitis.

Authors+Show Affiliations

Department of Conservative Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima, 770-8504, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19734466

Citation

Hirao, K, et al. "Roles of TLR2, TLR4, NOD2, and NOD1 in Pulp Fibroblasts." Journal of Dental Research, vol. 88, no. 8, 2009, pp. 762-7.
Hirao K, Yumoto H, Takahashi K, et al. Roles of TLR2, TLR4, NOD2, and NOD1 in pulp fibroblasts. J Dent Res. 2009;88(8):762-7.
Hirao, K., Yumoto, H., Takahashi, K., Mukai, K., Nakanishi, T., & Matsuo, T. (2009). Roles of TLR2, TLR4, NOD2, and NOD1 in pulp fibroblasts. Journal of Dental Research, 88(8), pp. 762-7. doi:10.1177/0022034509341779.
Hirao K, et al. Roles of TLR2, TLR4, NOD2, and NOD1 in Pulp Fibroblasts. J Dent Res. 2009;88(8):762-7. PubMed PMID: 19734466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Roles of TLR2, TLR4, NOD2, and NOD1 in pulp fibroblasts. AU - Hirao,K, AU - Yumoto,H, AU - Takahashi,K, AU - Mukai,K, AU - Nakanishi,T, AU - Matsuo,T, PY - 2009/9/8/entrez PY - 2009/9/8/pubmed PY - 2009/9/30/medline SP - 762 EP - 7 JF - Journal of dental research JO - J. Dent. Res. VL - 88 IS - 8 N2 - Pulp fibroblasts express various pro-inflammatory mediators leading to marked infiltration of inflammatory cells in the progression of pulpitis. We hypothesized that pulp fibroblasts play roles in the recognition of invaded caries-related bacteria and the subsequent innate immune responses. We found clear expressions of TLR2, NOD1, and NOD2 and a faint expression of TLR4 in human dental pulp fibroblasts (HDPF) by RT-PCR and flow cytometry. We also observed that various pro-inflammatory mediators, including cytokines, chemokines, adhesion molecules, prostaglandin E(2) and its key enzyme COX-2, not iNOS or caspase-1, were markedly up-regulated by stimulation with these TLR and NOD agonists. More over, the NOD2 agonist acted synergistically with the TLR2, not the TLR4, agonist to stimulate the production of pro-inflammatory mediators in HDPF. These findings indicate that TLR2, TLR4, NOD2, and NOD1 in HDPF are functional receptors, and NOD2 is a modulator of signals transmitted through TLR2 in pulpal immune responses, leading to progressive pulpitis. SN - 1544-0591 UR - https://www.unboundmedicine.com/medline/citation/19734466/Roles_of_TLR2_TLR4_NOD2_and_NOD1_in_pulp_fibroblasts_ L2 - http://journals.sagepub.com/doi/full/10.1177/0022034509341779?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -