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Exopolysaccharides produced by Bifidobacterium longum IPLA E44 and Bifidobacterium animalis subsp. lactis IPLA R1 modify the composition and metabolic activity of human faecal microbiota in pH-controlled batch cultures.
Int J Food Microbiol. 2009 Nov 15; 135(3):260-7.IJ

Abstract

Exopolysaccharides (EPS) isolated from two Bifidobacterium strains, one of human intestinal origin (Bifidobacterium longum subsp. longum IPLA E44) and the other from dairy origin (Bifidobacterium animalis subsp. lactis IPLA R1), were subjected to in vitro chemically simulated gastrointestinal digestion, which showed the absence of degradation of both polymers in these conditions. Polymers were then used as carbon sources in pH-controlled faecal batch cultures and compared with the non-prebiotic carbohydrate glucose and the prebiotic inulin to determine changes in the composition of faecal bacteria. A set of eight fluorescent in situ hybridisation oligonucleotide probes targeting 16S rRNA sequences was used to quantify specific groups of microorganisms. Growth of the opportunistic pathogen Clostridium histolyticum occurred with all carbohydrates tested similarly to that found in negative control cultures without added carbohydrate and was mainly attributed to the culture conditions used rather than enhancement of growth by these substrates. Polymers E44 and R1 stimulated growth of Lactobacillus/Enterococcus, Bifidobacterium, and Bacteroides/Prevotella in a similar way to that seen with inulin. The EPS R1 also promoted growth of the Atopobium cluster during the first 24h of fermentation. An increase in acetic and lactic acids was found during early stages of fermentation (first 10-24h) correlating with increases of Lactobacillus, Bifidobacterium, and Atopobium. Propionic acid concentrations increased in old cultures, which was coincident with the enrichment of Clostridium cluster IX in cultures with EPS R1 and with the increases in Bacteroides in cultures with both microbial EPS (R1 and E44) and inulin. The lowest acetic to propionic acid ratio was obtained for EPS E44. None of the carbohydrates tested supported the growth of microorganisms from Clostridium clusters XIVa+b and IV, results that correlate with the poor butyrate production in the presence of EPS. Thus, EPS synthesized by bifidobacteria from dairy and intestinal origins can modulate the intestinal microbiota in vitro, promoting changes in some numerically and metabolically relevant microbial populations and shifts in the production of short chain fatty acids.

Authors+Show Affiliations

Department of Food Biosciences, The University of Reading, Reading, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19735956

Citation

Salazar, Nuria, et al. "Exopolysaccharides Produced By Bifidobacterium Longum IPLA E44 and Bifidobacterium Animalis Subsp. Lactis IPLA R1 Modify the Composition and Metabolic Activity of Human Faecal Microbiota in pH-controlled Batch Cultures." International Journal of Food Microbiology, vol. 135, no. 3, 2009, pp. 260-7.
Salazar N, Ruas-Madiedo P, Kolida S, et al. Exopolysaccharides produced by Bifidobacterium longum IPLA E44 and Bifidobacterium animalis subsp. lactis IPLA R1 modify the composition and metabolic activity of human faecal microbiota in pH-controlled batch cultures. Int J Food Microbiol. 2009;135(3):260-7.
Salazar, N., Ruas-Madiedo, P., Kolida, S., Collins, M., Rastall, R., Gibson, G., & de Los Reyes-Gavilán, C. G. (2009). Exopolysaccharides produced by Bifidobacterium longum IPLA E44 and Bifidobacterium animalis subsp. lactis IPLA R1 modify the composition and metabolic activity of human faecal microbiota in pH-controlled batch cultures. International Journal of Food Microbiology, 135(3), 260-7. https://doi.org/10.1016/j.ijfoodmicro.2009.08.017
Salazar N, et al. Exopolysaccharides Produced By Bifidobacterium Longum IPLA E44 and Bifidobacterium Animalis Subsp. Lactis IPLA R1 Modify the Composition and Metabolic Activity of Human Faecal Microbiota in pH-controlled Batch Cultures. Int J Food Microbiol. 2009 Nov 15;135(3):260-7. PubMed PMID: 19735956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exopolysaccharides produced by Bifidobacterium longum IPLA E44 and Bifidobacterium animalis subsp. lactis IPLA R1 modify the composition and metabolic activity of human faecal microbiota in pH-controlled batch cultures. AU - Salazar,Nuria, AU - Ruas-Madiedo,Patricia, AU - Kolida,Sofia, AU - Collins,Michelle, AU - Rastall,Robert, AU - Gibson,Glenn, AU - de Los Reyes-Gavilán,Clara G, Y1 - 2009/08/19/ PY - 2009/04/07/received PY - 2009/06/19/revised PY - 2009/08/10/accepted PY - 2009/9/9/entrez PY - 2009/9/9/pubmed PY - 2010/2/3/medline SP - 260 EP - 7 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 135 IS - 3 N2 - Exopolysaccharides (EPS) isolated from two Bifidobacterium strains, one of human intestinal origin (Bifidobacterium longum subsp. longum IPLA E44) and the other from dairy origin (Bifidobacterium animalis subsp. lactis IPLA R1), were subjected to in vitro chemically simulated gastrointestinal digestion, which showed the absence of degradation of both polymers in these conditions. Polymers were then used as carbon sources in pH-controlled faecal batch cultures and compared with the non-prebiotic carbohydrate glucose and the prebiotic inulin to determine changes in the composition of faecal bacteria. A set of eight fluorescent in situ hybridisation oligonucleotide probes targeting 16S rRNA sequences was used to quantify specific groups of microorganisms. Growth of the opportunistic pathogen Clostridium histolyticum occurred with all carbohydrates tested similarly to that found in negative control cultures without added carbohydrate and was mainly attributed to the culture conditions used rather than enhancement of growth by these substrates. Polymers E44 and R1 stimulated growth of Lactobacillus/Enterococcus, Bifidobacterium, and Bacteroides/Prevotella in a similar way to that seen with inulin. The EPS R1 also promoted growth of the Atopobium cluster during the first 24h of fermentation. An increase in acetic and lactic acids was found during early stages of fermentation (first 10-24h) correlating with increases of Lactobacillus, Bifidobacterium, and Atopobium. Propionic acid concentrations increased in old cultures, which was coincident with the enrichment of Clostridium cluster IX in cultures with EPS R1 and with the increases in Bacteroides in cultures with both microbial EPS (R1 and E44) and inulin. The lowest acetic to propionic acid ratio was obtained for EPS E44. None of the carbohydrates tested supported the growth of microorganisms from Clostridium clusters XIVa+b and IV, results that correlate with the poor butyrate production in the presence of EPS. Thus, EPS synthesized by bifidobacteria from dairy and intestinal origins can modulate the intestinal microbiota in vitro, promoting changes in some numerically and metabolically relevant microbial populations and shifts in the production of short chain fatty acids. SN - 1879-3460 UR - https://www.unboundmedicine.com/medline/citation/19735956/Exopolysaccharides_produced_by_Bifidobacterium_longum_IPLA_E44_and_Bifidobacterium_animalis_subsp__lactis_IPLA_R1_modify_the_composition_and_metabolic_activity_of_human_faecal_microbiota_in_pH_controlled_batch_cultures_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(09)00423-1 DB - PRIME DP - Unbound Medicine ER -