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Impaired immune response in severe human lower tract respiratory infection by respiratory syncytial virus.
Pediatr Infect Dis J. 2009 Oct; 28(10):867-73.PI

Abstract

BACKGROUND

Respiratory syncytial virus (RSV) is a major cause of acute lower respiratory infection in infants. The immune response plays a leading role in the severity of the disease. We hypothesized that severe RSV disease is associated with an impaired immune response characterized by low circulating T lymphocytes and plasma cytokine concentrations.

METHODS

We evaluate the in vivo immune responses of previously healthy infants with their first proven RSV-acute lower respiratory infection that required hospitalization. According to the clinical severity, defined by using a strict scoring system, the in vivo immune response was compared through the analysis of plasma cytokine values and the phenotyping of peripheral blood lymphocyte and natural killer (NK) cells.

RESULTS

Absolute blood cell counts of CD4+, CD8+, and CD19+ lymphocytes and NK cells were lower in subjects with RSV than in control infants. Lowest cell counts were observed in more severe RSV-infected infants. Significant low values were obtained in CD8+ lymphocytes (P = 0.03) and nonactive NK cells, that express CD94 antigen (P = 0.046). In contrast, activated NK cells that do not express CD94 molecules were significantly higher in RSV infected infants than in healthy controls (% of cells: P = 0.004). The interferon-gamma and tumor necrosis factor-alpha values in RSV infected patients were lower than in controls subjects. Interleukin-17 cytokine was not detected in healthy infants and the largest concentration was found in moderately ill patients as compared with severe cases (P = 0.033). RSV infection showed significantly higher interleukin-8 chemokine than in control infants (P = 0.024).

CONCLUSION

We propose that severe RSV infection in very young infants is associated with poor blood proinflammatory cytokine production, low counts of CD8+ T cells and with a greater activity of a group of NK cells, that are independent of the major histocompatibility complex class Ib recognition system.

Authors+Show Affiliations

Programa de Virologia, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile. clarrana@med.uchile.clNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19738511

Citation

Larrañaga, Carmen L., et al. "Impaired Immune Response in Severe Human Lower Tract Respiratory Infection By Respiratory Syncytial Virus." The Pediatric Infectious Disease Journal, vol. 28, no. 10, 2009, pp. 867-73.
Larrañaga CL, Ampuero SL, Luchsinger VF, et al. Impaired immune response in severe human lower tract respiratory infection by respiratory syncytial virus. Pediatr Infect Dis J. 2009;28(10):867-73.
Larrañaga, C. L., Ampuero, S. L., Luchsinger, V. F., Carrión, F. A., Aguilar, N. V., Morales, P. R., Palomino, M. A., Tapia, L. F., & Avendaño, L. F. (2009). Impaired immune response in severe human lower tract respiratory infection by respiratory syncytial virus. The Pediatric Infectious Disease Journal, 28(10), 867-73. https://doi.org/10.1097/INF.0b013e3181a3ea71
Larrañaga CL, et al. Impaired Immune Response in Severe Human Lower Tract Respiratory Infection By Respiratory Syncytial Virus. Pediatr Infect Dis J. 2009;28(10):867-73. PubMed PMID: 19738511.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impaired immune response in severe human lower tract respiratory infection by respiratory syncytial virus. AU - Larrañaga,Carmen L, AU - Ampuero,Sandra L, AU - Luchsinger,Vivian F, AU - Carrión,Flavio A, AU - Aguilar,Nelson V, AU - Morales,Pamela R, AU - Palomino,María Angélica M, AU - Tapia,Lorena F, AU - Avendaño,Luis F, PY - 2009/9/10/entrez PY - 2009/9/10/pubmed PY - 2010/3/31/medline SP - 867 EP - 73 JF - The Pediatric infectious disease journal JO - Pediatr Infect Dis J VL - 28 IS - 10 N2 - BACKGROUND: Respiratory syncytial virus (RSV) is a major cause of acute lower respiratory infection in infants. The immune response plays a leading role in the severity of the disease. We hypothesized that severe RSV disease is associated with an impaired immune response characterized by low circulating T lymphocytes and plasma cytokine concentrations. METHODS: We evaluate the in vivo immune responses of previously healthy infants with their first proven RSV-acute lower respiratory infection that required hospitalization. According to the clinical severity, defined by using a strict scoring system, the in vivo immune response was compared through the analysis of plasma cytokine values and the phenotyping of peripheral blood lymphocyte and natural killer (NK) cells. RESULTS: Absolute blood cell counts of CD4+, CD8+, and CD19+ lymphocytes and NK cells were lower in subjects with RSV than in control infants. Lowest cell counts were observed in more severe RSV-infected infants. Significant low values were obtained in CD8+ lymphocytes (P = 0.03) and nonactive NK cells, that express CD94 antigen (P = 0.046). In contrast, activated NK cells that do not express CD94 molecules were significantly higher in RSV infected infants than in healthy controls (% of cells: P = 0.004). The interferon-gamma and tumor necrosis factor-alpha values in RSV infected patients were lower than in controls subjects. Interleukin-17 cytokine was not detected in healthy infants and the largest concentration was found in moderately ill patients as compared with severe cases (P = 0.033). RSV infection showed significantly higher interleukin-8 chemokine than in control infants (P = 0.024). CONCLUSION: We propose that severe RSV infection in very young infants is associated with poor blood proinflammatory cytokine production, low counts of CD8+ T cells and with a greater activity of a group of NK cells, that are independent of the major histocompatibility complex class Ib recognition system. SN - 1532-0987 UR - https://www.unboundmedicine.com/medline/citation/19738511/Impaired_immune_response_in_severe_human_lower_tract_respiratory_infection_by_respiratory_syncytial_virus_ L2 - https://doi.org/10.1097/INF.0b013e3181a3ea71 DB - PRIME DP - Unbound Medicine ER -