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Expansion of FOXP3-positive CD4+CD25+ T cells associated with disease activity in atopic dermatitis.
Ann Allergy Asthma Immunol. 2009 Aug; 103(2):160-5.AA

Abstract

BACKGROUND

FOXP3-positive CD4+CD25+ T cells are known to have an immunoregulatory function by means of preventing T-cell reactivity to both self- and non-self-antigens. However, the role of these cells in the pathogenesis of allergic diseases is not clear.

OBJECTIVE

To evaluate the quantity and quality of circulating FOXP3-positive T cells in patients with atopic dermatitis (AD).

METHODS

Peripheral blood mononuclear cells were isolated from 35 AD patients (mean [SD] age, 27.1 [7.5] years) and 36 controls (mean [SD] age, 27.5 [10.0] years). Cellular FOXP3 expression was analyzed using flow cytometry. Characteristics of FOXP3-positive T cells were evaluated with respect to cytokine production capability and suppressive function.

RESULTS

Frequencies of circulating FOXP3+CD25+ cells in the CD4+ T-cell population of AD patients were significantly higher than those in controls (mean [SD], 7.4% [4.6%] vs 4.5% [1.3%]; P = .002) and correlated with their Scoring Atopic Dermatitis (SCORAD) scores (r = 0.74, P = .008) and peripheral blood eosinophil counts (r = 0.72, P < .001). In the patients whose samples were analyzed at intervals of 1 to 2 months, frequencies of FOXP3-positive T cells were decreased as their skin lesions improved, regardless of medicines used. FOXP3-positive CD4+ T cells from patients, as well as those from controls, showed little capability to synthesize interferon gamma and interleukin 4. No differences were found in suppression abilities of CD4+CD25+ T cells between AD patients and controls.

CONCLUSIONS

Our data suggest that dynamic fluctuation in numbers of circulating FOXP3-positive regulatory T cells might contribute to the pathogenesis of AD.

Authors+Show Affiliations

Department of Pediatrics, Graduate School of Medicine, University of Toyama, Toyama, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19739430

Citation

Ito, Yasunori, et al. "Expansion of FOXP3-positive CD4+CD25+ T Cells Associated With Disease Activity in Atopic Dermatitis." Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology, vol. 103, no. 2, 2009, pp. 160-5.
Ito Y, Adachi Y, Makino T, et al. Expansion of FOXP3-positive CD4+CD25+ T cells associated with disease activity in atopic dermatitis. Ann Allergy Asthma Immunol. 2009;103(2):160-5.
Ito, Y., Adachi, Y., Makino, T., Higashiyama, H., Fuchizawa, T., Shimizu, T., & Miyawaki, T. (2009). Expansion of FOXP3-positive CD4+CD25+ T cells associated with disease activity in atopic dermatitis. Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology, 103(2), 160-5. https://doi.org/10.1016/S1081-1206(10)60170-6
Ito Y, et al. Expansion of FOXP3-positive CD4+CD25+ T Cells Associated With Disease Activity in Atopic Dermatitis. Ann Allergy Asthma Immunol. 2009;103(2):160-5. PubMed PMID: 19739430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expansion of FOXP3-positive CD4+CD25+ T cells associated with disease activity in atopic dermatitis. AU - Ito,Yasunori, AU - Adachi,Yuichi, AU - Makino,Teruhiko, AU - Higashiyama,Hiroyuki, AU - Fuchizawa,Tatsuya, AU - Shimizu,Tadamichi, AU - Miyawaki,Toshio, PY - 2009/9/11/entrez PY - 2009/9/11/pubmed PY - 2009/10/3/medline SP - 160 EP - 5 JF - Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology JO - Ann Allergy Asthma Immunol VL - 103 IS - 2 N2 - BACKGROUND: FOXP3-positive CD4+CD25+ T cells are known to have an immunoregulatory function by means of preventing T-cell reactivity to both self- and non-self-antigens. However, the role of these cells in the pathogenesis of allergic diseases is not clear. OBJECTIVE: To evaluate the quantity and quality of circulating FOXP3-positive T cells in patients with atopic dermatitis (AD). METHODS: Peripheral blood mononuclear cells were isolated from 35 AD patients (mean [SD] age, 27.1 [7.5] years) and 36 controls (mean [SD] age, 27.5 [10.0] years). Cellular FOXP3 expression was analyzed using flow cytometry. Characteristics of FOXP3-positive T cells were evaluated with respect to cytokine production capability and suppressive function. RESULTS: Frequencies of circulating FOXP3+CD25+ cells in the CD4+ T-cell population of AD patients were significantly higher than those in controls (mean [SD], 7.4% [4.6%] vs 4.5% [1.3%]; P = .002) and correlated with their Scoring Atopic Dermatitis (SCORAD) scores (r = 0.74, P = .008) and peripheral blood eosinophil counts (r = 0.72, P < .001). In the patients whose samples were analyzed at intervals of 1 to 2 months, frequencies of FOXP3-positive T cells were decreased as their skin lesions improved, regardless of medicines used. FOXP3-positive CD4+ T cells from patients, as well as those from controls, showed little capability to synthesize interferon gamma and interleukin 4. No differences were found in suppression abilities of CD4+CD25+ T cells between AD patients and controls. CONCLUSIONS: Our data suggest that dynamic fluctuation in numbers of circulating FOXP3-positive regulatory T cells might contribute to the pathogenesis of AD. SN - 1081-1206 UR - https://www.unboundmedicine.com/medline/citation/19739430/Expansion_of_FOXP3_positive_CD4+CD25+_T_cells_associated_with_disease_activity_in_atopic_dermatitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1081-1206(10)60170-6 DB - PRIME DP - Unbound Medicine ER -