Tags

Type your tag names separated by a space and hit enter

Dynamics of the CD8 T-cell response following yellow fever virus 17D immunization.
Immunology. 2009 Sep; 128(1 Suppl):e718-27.I

Abstract

Management of yellow fever is focused on the prevention of illness by the use of the yellow fever virus (YFV) 17D vaccine. The role of neutralizing antibodies in protection is generally accepted with YFV-specific T cells likely contributing to the control of viral replication. We studied CD8(+) T-cell responses to four defined human leucocyte antigen-B35-restricted epitopes in YFV vaccine recipients as a model of the kinetics of cytotoxic T-lymphocyte responses to an acute human viral infection. Multiple features of these epitope-specific responses were analysed after vaccination including magnitude, cytokine production, phenotype and T-cell receptor repertoire. Peak peptide-specific interferon-gamma (IFN-gamma) responses of almost 1% of CD8(+) T cells were seen as early as 2 weeks post-vaccination; however, dominant responses varied between donors. Peptide-specific responses were still detectable at 54 months post-vaccination. Tetramer-positive cells, at high frequencies, were detected as early as 7-9 days, before detectable IFN-gamma-producing cells, suggesting a defect in the functional capacity of some antigen-specific cells early post-vaccination. The predominant memory phenotype of the tetramer-positive population was a differentiated effector (CD45RA(+) CCR7(-) CD62L(-)) phenotype. The T-cell receptor Vbeta analysis revealed a diverse oligoclonal repertoire in tetramer-positive T-cell populations in two individuals. These characteristics of the YFV-specific T-cell response could contribute to vaccine effectiveness.

Authors+Show Affiliations

Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, MA 01655, USA. mary.co@umassmed.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19740333

Citation

Co, Mary Dawn T., et al. "Dynamics of the CD8 T-cell Response Following Yellow Fever Virus 17D Immunization." Immunology, vol. 128, no. 1 Suppl, 2009, pp. e718-27.
Co MD, Kilpatrick ED, Rothman AL. Dynamics of the CD8 T-cell response following yellow fever virus 17D immunization. Immunology. 2009;128(1 Suppl):e718-27.
Co, M. D., Kilpatrick, E. D., & Rothman, A. L. (2009). Dynamics of the CD8 T-cell response following yellow fever virus 17D immunization. Immunology, 128(1 Suppl), e718-27. https://doi.org/10.1111/j.1365-2567.2009.03070.x
Co MD, Kilpatrick ED, Rothman AL. Dynamics of the CD8 T-cell Response Following Yellow Fever Virus 17D Immunization. Immunology. 2009;128(1 Suppl):e718-27. PubMed PMID: 19740333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamics of the CD8 T-cell response following yellow fever virus 17D immunization. AU - Co,Mary Dawn T, AU - Kilpatrick,Elizabeth D, AU - Rothman,Alan L, Y1 - 2009/02/11/ PY - 2009/9/11/entrez PY - 2009/9/25/pubmed PY - 2010/1/26/medline SP - e718 EP - 27 JF - Immunology JO - Immunology VL - 128 IS - 1 Suppl N2 - Management of yellow fever is focused on the prevention of illness by the use of the yellow fever virus (YFV) 17D vaccine. The role of neutralizing antibodies in protection is generally accepted with YFV-specific T cells likely contributing to the control of viral replication. We studied CD8(+) T-cell responses to four defined human leucocyte antigen-B35-restricted epitopes in YFV vaccine recipients as a model of the kinetics of cytotoxic T-lymphocyte responses to an acute human viral infection. Multiple features of these epitope-specific responses were analysed after vaccination including magnitude, cytokine production, phenotype and T-cell receptor repertoire. Peak peptide-specific interferon-gamma (IFN-gamma) responses of almost 1% of CD8(+) T cells were seen as early as 2 weeks post-vaccination; however, dominant responses varied between donors. Peptide-specific responses were still detectable at 54 months post-vaccination. Tetramer-positive cells, at high frequencies, were detected as early as 7-9 days, before detectable IFN-gamma-producing cells, suggesting a defect in the functional capacity of some antigen-specific cells early post-vaccination. The predominant memory phenotype of the tetramer-positive population was a differentiated effector (CD45RA(+) CCR7(-) CD62L(-)) phenotype. The T-cell receptor Vbeta analysis revealed a diverse oligoclonal repertoire in tetramer-positive T-cell populations in two individuals. These characteristics of the YFV-specific T-cell response could contribute to vaccine effectiveness. SN - 1365-2567 UR - https://www.unboundmedicine.com/medline/citation/19740333/Dynamics_of_the_CD8_T_cell_response_following_yellow_fever_virus_17D_immunization_ L2 - https://doi.org/10.1111/j.1365-2567.2009.03070.x DB - PRIME DP - Unbound Medicine ER -