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A sensitive DNA biosensor fabricated with gold nanoparticles/poly (p-aminobenzoic acid)/carbon nanotubes modified electrode.
Colloids Surf B Biointerfaces. 2010 Jan 01; 75(1):179-85.CS

Abstract

In this work, we fabricated a sensitive electrochemical DNA biosensor for the detection of target DNA. Aminobenzoic acid (ABA) was firstly electropolymerized on the surface of the glassy carbon electrode (GCE) modified with multi-walled carbon nanotubes with carboxyl groups (MWCNTs) by cyclic voltammetry (CV). Gold nanoparticles (AuNPs) were subsequently introduced to the surface of PABA-MWNTs composite film by electrochemical deposition mode. Probe DNA was immobilized on the surface of AuNPs through Au-S bond. Scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectra (EIS) were used to investigate the film assembly process. Differential pulse voltammetry (DPV) was used to monitor DNA hybridization event by measurement of the intercalated adriamycin. Under the optimal conditions, the increase of reduction peak current of adriamycin was linear with the logarithm of the concentration of the complementary oligonucleotides from 1.0 x 10(-12) to 5.0 x 10(-9)M with a detection limit of 3.5 x 10(-13)M. This DNA biosensor has a good stability and reproducibility.

Authors+Show Affiliations

College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Normal University, Wuhu 241000, PR China. zhyz65@mail.ahnu.edu.cnNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19740633

Citation

Zhang, Yuzhong, et al. "A Sensitive DNA Biosensor Fabricated With Gold Nanoparticles/poly (p-aminobenzoic Acid)/carbon Nanotubes Modified Electrode." Colloids and Surfaces. B, Biointerfaces, vol. 75, no. 1, 2010, pp. 179-85.
Zhang Y, Wang J, Xu M. A sensitive DNA biosensor fabricated with gold nanoparticles/poly (p-aminobenzoic acid)/carbon nanotubes modified electrode. Colloids Surf B Biointerfaces. 2010;75(1):179-85.
Zhang, Y., Wang, J., & Xu, M. (2010). A sensitive DNA biosensor fabricated with gold nanoparticles/poly (p-aminobenzoic acid)/carbon nanotubes modified electrode. Colloids and Surfaces. B, Biointerfaces, 75(1), 179-85. https://doi.org/10.1016/j.colsurfb.2009.08.030
Zhang Y, Wang J, Xu M. A Sensitive DNA Biosensor Fabricated With Gold Nanoparticles/poly (p-aminobenzoic Acid)/carbon Nanotubes Modified Electrode. Colloids Surf B Biointerfaces. 2010 Jan 1;75(1):179-85. PubMed PMID: 19740633.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A sensitive DNA biosensor fabricated with gold nanoparticles/poly (p-aminobenzoic acid)/carbon nanotubes modified electrode. AU - Zhang,Yuzhong, AU - Wang,Jie, AU - Xu,Minglu, Y1 - 2009/08/25/ PY - 2009/06/25/received PY - 2009/08/04/revised PY - 2009/08/18/accepted PY - 2009/9/11/entrez PY - 2009/9/11/pubmed PY - 2010/2/18/medline SP - 179 EP - 85 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 75 IS - 1 N2 - In this work, we fabricated a sensitive electrochemical DNA biosensor for the detection of target DNA. Aminobenzoic acid (ABA) was firstly electropolymerized on the surface of the glassy carbon electrode (GCE) modified with multi-walled carbon nanotubes with carboxyl groups (MWCNTs) by cyclic voltammetry (CV). Gold nanoparticles (AuNPs) were subsequently introduced to the surface of PABA-MWNTs composite film by electrochemical deposition mode. Probe DNA was immobilized on the surface of AuNPs through Au-S bond. Scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectra (EIS) were used to investigate the film assembly process. Differential pulse voltammetry (DPV) was used to monitor DNA hybridization event by measurement of the intercalated adriamycin. Under the optimal conditions, the increase of reduction peak current of adriamycin was linear with the logarithm of the concentration of the complementary oligonucleotides from 1.0 x 10(-12) to 5.0 x 10(-9)M with a detection limit of 3.5 x 10(-13)M. This DNA biosensor has a good stability and reproducibility. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/19740633/A_sensitive_DNA_biosensor_fabricated_with_gold_nanoparticles/poly__p_aminobenzoic_acid_/carbon_nanotubes_modified_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(09)00389-0 DB - PRIME DP - Unbound Medicine ER -