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SERS detection of indirect viral DNA capture using colloidal gold and methylene blue as a Raman label.
Biosens Bioelectron. 2009 Dec 15; 25(4):674-81.BB

Abstract

An indirect capture model assay using colloidal Au nanoparticles is demonstrated for surface enhanced Raman scattering (SERS) spectroscopy detection of DNA. The sequence targeted for capture was derived from the West Nile Virus (WNV) RNA genome and selected on the basis of exhibiting minimal secondary structure formation. Upon incubation with colloidal Au, hybridization complexes containing the WNV target sequence, a complementary capture oligonucleotide conjugated to a strong tethering group and a complementary reporter oligonucleotide conjugated to methylene blue (MB), a Raman label, anchors the resultant ternary complex to Au nanoparticles and positions MB within the required sensing distance for SERS enhancement. The subsequent elicitation of surface enhanced plasmon resonance by laser excitation provides a spectral peak signature profile that is capture-specific and characteristic of the Raman spectrum for MB. Detection sensitivity is in the submicromolar range and was shown to be highest for thiol, and less so for amino, modifications at the 5' terminus of the capture oligonucleotide. Finally, using Quartz Crystal Microbalance-Dissipation as a tool for modeling ternary complex binding to Au surfaces, quantitative measurements of surface mass coverage on Au plated sensor crystals established a positive correlation between levels of ternary complex adsorption and their correspondent levels of SERS signal intensification. Adapted to a compact Raman spectrometer, which is designed for analyte detection in capillary tubes, this assay provides a rapid, mobile and cost effective alternative to expensive spectroscopic instrumentation, which is often restricted to analytical laboratories.

Authors+Show Affiliations

Arthropod-Borne Animal Diseases Research Laboratory, USDA/ARS, Laramie, WY 82071, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19740646

Citation

Harpster, Mark H., et al. "SERS Detection of Indirect Viral DNA Capture Using Colloidal Gold and Methylene Blue as a Raman Label." Biosensors & Bioelectronics, vol. 25, no. 4, 2009, pp. 674-81.
Harpster MH, Zhang H, Sankara-Warrier AK, et al. SERS detection of indirect viral DNA capture using colloidal gold and methylene blue as a Raman label. Biosens Bioelectron. 2009;25(4):674-81.
Harpster, M. H., Zhang, H., Sankara-Warrier, A. K., Ray, B. H., Ward, T. R., Kollmar, J. P., Carron, K. T., Mecham, J. O., Corcoran, R. C., Wilson, W. C., & Johnson, P. A. (2009). SERS detection of indirect viral DNA capture using colloidal gold and methylene blue as a Raman label. Biosensors & Bioelectronics, 25(4), 674-81. https://doi.org/10.1016/j.bios.2009.05.020
Harpster MH, et al. SERS Detection of Indirect Viral DNA Capture Using Colloidal Gold and Methylene Blue as a Raman Label. Biosens Bioelectron. 2009 Dec 15;25(4):674-81. PubMed PMID: 19740646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SERS detection of indirect viral DNA capture using colloidal gold and methylene blue as a Raman label. AU - Harpster,Mark H, AU - Zhang,Hao, AU - Sankara-Warrier,Ajaya K, AU - Ray,Bryan H, AU - Ward,Timothy R, AU - Kollmar,J Pablo, AU - Carron,Keith T, AU - Mecham,James O, AU - Corcoran,Robert C, AU - Wilson,William C, AU - Johnson,Patrick A, Y1 - 2009/05/27/ PY - 2009/05/15/received PY - 2009/05/19/accepted PY - 2009/9/11/entrez PY - 2009/9/11/pubmed PY - 2010/1/14/medline SP - 674 EP - 81 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 25 IS - 4 N2 - An indirect capture model assay using colloidal Au nanoparticles is demonstrated for surface enhanced Raman scattering (SERS) spectroscopy detection of DNA. The sequence targeted for capture was derived from the West Nile Virus (WNV) RNA genome and selected on the basis of exhibiting minimal secondary structure formation. Upon incubation with colloidal Au, hybridization complexes containing the WNV target sequence, a complementary capture oligonucleotide conjugated to a strong tethering group and a complementary reporter oligonucleotide conjugated to methylene blue (MB), a Raman label, anchors the resultant ternary complex to Au nanoparticles and positions MB within the required sensing distance for SERS enhancement. The subsequent elicitation of surface enhanced plasmon resonance by laser excitation provides a spectral peak signature profile that is capture-specific and characteristic of the Raman spectrum for MB. Detection sensitivity is in the submicromolar range and was shown to be highest for thiol, and less so for amino, modifications at the 5' terminus of the capture oligonucleotide. Finally, using Quartz Crystal Microbalance-Dissipation as a tool for modeling ternary complex binding to Au surfaces, quantitative measurements of surface mass coverage on Au plated sensor crystals established a positive correlation between levels of ternary complex adsorption and their correspondent levels of SERS signal intensification. Adapted to a compact Raman spectrometer, which is designed for analyte detection in capillary tubes, this assay provides a rapid, mobile and cost effective alternative to expensive spectroscopic instrumentation, which is often restricted to analytical laboratories. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19740646/SERS_detection_of_indirect_viral_DNA_capture_using_colloidal_gold_and_methylene_blue_as_a_Raman_label_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(09)00291-7 DB - PRIME DP - Unbound Medicine ER -