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Fruit extracts of Momordica charantia potentiate glucose uptake and up-regulate Glut-4, PPAR gamma and PI3K.
J Ethnopharmacol. 2009 Dec 10; 126(3):533-7.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Momordica charantia fruit is a widely used traditional medicinal herb as, anti-diabetic, anti-HIV, anti-ulcer, anti-inflammatory, anti-leukemic, anti-microbial, and anti-tumor.

AIMS OF STUDY

The present study is undertaken to investigate the possible mode of action of fruit extracts derived from Momordica charantia (MC) and study its pharmacological effects for controlling diabetic mellitus. Effects of aqueous and chloroform extracts of Momordica charantia fruit on glucose uptake and up-regulation of glucose transporter (Glut-4), peroxisome proliferator activator receptor gamma (PPAR gamma) and phosphatidylinositol-3 kinase (PI3K), were investigated to show its efficacy as a hypoglycaemic agent.

MATERIALS AND METHODS

Dose dependent glucose uptake assay was performed on L6 myotubes using 2-deoxy-D-[1-(3)H] glucose. Up-regulatory effects of the extracts on the mRNA expression level of Glut-4, PPAR gamma and PI3K have been studied.

RESULTS

The association of Momordica charantia with the aqueous and chloroform extracts of Momordica charantia fruit at 6 microg/ml has shown significant up-regulatory effect, respectively, by 3.6-, 2.8- and 3.8-fold on the battery of targets Glut-4, PPAR gamma and PI3K involved in glucose transport. The up-regulation of glucose uptake was comparable with insulin and rosiglitazone which was approximately 2-fold over the control. Moreover, the inhibitory effect of the cyclohexamide on Momordica charantia fruit extract mediated glucose uptake suggested the requirement of new protein synthesis for the enhanced glucose uptake.

CONCLUSION

This study demonstrated the significance of Glut-4, PPAR gamma and PI3K up-regulation by Momordica charantia in augmenting the glucose uptake and homeostasis.

Authors+Show Affiliations

Bio-products Laboratory, Central Leather Research Institute, Council of Scientific and Industrial Research, Adyar, Chennai 600 020, Tamil Nadu, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19744549

Citation

Kumar, Ramadhar, et al. "Fruit Extracts of Momordica Charantia Potentiate Glucose Uptake and Up-regulate Glut-4, PPAR Gamma and PI3K." Journal of Ethnopharmacology, vol. 126, no. 3, 2009, pp. 533-7.
Kumar R, Balaji S, Uma TS, et al. Fruit extracts of Momordica charantia potentiate glucose uptake and up-regulate Glut-4, PPAR gamma and PI3K. J Ethnopharmacol. 2009;126(3):533-7.
Kumar, R., Balaji, S., Uma, T. S., & Sehgal, P. K. (2009). Fruit extracts of Momordica charantia potentiate glucose uptake and up-regulate Glut-4, PPAR gamma and PI3K. Journal of Ethnopharmacology, 126(3), 533-7. https://doi.org/10.1016/j.jep.2009.08.048
Kumar R, et al. Fruit Extracts of Momordica Charantia Potentiate Glucose Uptake and Up-regulate Glut-4, PPAR Gamma and PI3K. J Ethnopharmacol. 2009 Dec 10;126(3):533-7. PubMed PMID: 19744549.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fruit extracts of Momordica charantia potentiate glucose uptake and up-regulate Glut-4, PPAR gamma and PI3K. AU - Kumar,Ramadhar, AU - Balaji,S, AU - Uma,T S, AU - Sehgal,P K, Y1 - 2009/09/08/ PY - 2008/12/21/received PY - 2009/07/17/revised PY - 2009/08/31/accepted PY - 2009/9/12/entrez PY - 2009/9/12/pubmed PY - 2010/2/19/medline SP - 533 EP - 7 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 126 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Momordica charantia fruit is a widely used traditional medicinal herb as, anti-diabetic, anti-HIV, anti-ulcer, anti-inflammatory, anti-leukemic, anti-microbial, and anti-tumor. AIMS OF STUDY: The present study is undertaken to investigate the possible mode of action of fruit extracts derived from Momordica charantia (MC) and study its pharmacological effects for controlling diabetic mellitus. Effects of aqueous and chloroform extracts of Momordica charantia fruit on glucose uptake and up-regulation of glucose transporter (Glut-4), peroxisome proliferator activator receptor gamma (PPAR gamma) and phosphatidylinositol-3 kinase (PI3K), were investigated to show its efficacy as a hypoglycaemic agent. MATERIALS AND METHODS: Dose dependent glucose uptake assay was performed on L6 myotubes using 2-deoxy-D-[1-(3)H] glucose. Up-regulatory effects of the extracts on the mRNA expression level of Glut-4, PPAR gamma and PI3K have been studied. RESULTS: The association of Momordica charantia with the aqueous and chloroform extracts of Momordica charantia fruit at 6 microg/ml has shown significant up-regulatory effect, respectively, by 3.6-, 2.8- and 3.8-fold on the battery of targets Glut-4, PPAR gamma and PI3K involved in glucose transport. The up-regulation of glucose uptake was comparable with insulin and rosiglitazone which was approximately 2-fold over the control. Moreover, the inhibitory effect of the cyclohexamide on Momordica charantia fruit extract mediated glucose uptake suggested the requirement of new protein synthesis for the enhanced glucose uptake. CONCLUSION: This study demonstrated the significance of Glut-4, PPAR gamma and PI3K up-regulation by Momordica charantia in augmenting the glucose uptake and homeostasis. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19744549/Fruit_extracts_of_Momordica_charantia_potentiate_glucose_uptake_and_up_regulate_Glut_4_PPAR_gamma_and_PI3K_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00559-5 DB - PRIME DP - Unbound Medicine ER -