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The application of ultrasound energy to increase lipid extraction throughput of solid matrix samples (flaxseed).
Prostaglandins Leukot Essent Fatty Acids. 2009 Nov-Dec; 81(5-6):417-23.PL

Abstract

Ultrasound may reduce lipid extraction times and increase analysis throughput of food materials. Ground flaxseed (25mg aliquots) were extracted in quadruplicate in 2:1 (v:v) chloroform:methanol, 3:2 hexane:isopropanol, 1:1 diethyl:petroleum ether or hexane with exposure to sonication at low frequencies of 20kHz with a 600W ultrasonic processor. Power was automatically varied to maintain constant amplitudes of 20%, 60% and 100% of 240microm for sonication exposures for 5, 10 and 20min, respectively. Total lipid dry weights and quantitative and qualitative fatty acids were determined. Results were compared to a standard 24-h, Folch-based, 2:1 chloroform:methanol extraction. Longer time exposures and higher sonication amplitudes were associated with increases in lipid recoveries. In particular, ultrasound-assisted extraction in 3:2 hexane:isopropanol for only 10min resulted in lipid and fatty acid recoveries similar to the 24-h standard method. Comprehensive testing on a variety of sample matrices and food products is required, but lipid extraction by ultrasound has potential to reduce sample processing time.

Authors+Show Affiliations

Laboratory of Nutritional and Nutraceutical Research, Department of Kinesiology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada, N2L 3G1.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19744844

Citation

Metherel, Adam H., et al. "The Application of Ultrasound Energy to Increase Lipid Extraction Throughput of Solid Matrix Samples (flaxseed)." Prostaglandins, Leukotrienes, and Essential Fatty Acids, vol. 81, no. 5-6, 2009, pp. 417-23.
Metherel AH, Taha AY, Izadi H, et al. The application of ultrasound energy to increase lipid extraction throughput of solid matrix samples (flaxseed). Prostaglandins Leukot Essent Fatty Acids. 2009;81(5-6):417-23.
Metherel, A. H., Taha, A. Y., Izadi, H., & Stark, K. D. (2009). The application of ultrasound energy to increase lipid extraction throughput of solid matrix samples (flaxseed). Prostaglandins, Leukotrienes, and Essential Fatty Acids, 81(5-6), 417-23. https://doi.org/10.1016/j.plefa.2009.07.003
Metherel AH, et al. The Application of Ultrasound Energy to Increase Lipid Extraction Throughput of Solid Matrix Samples (flaxseed). Prostaglandins Leukot Essent Fatty Acids. 2009 Nov-Dec;81(5-6):417-23. PubMed PMID: 19744844.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The application of ultrasound energy to increase lipid extraction throughput of solid matrix samples (flaxseed). AU - Metherel,Adam H, AU - Taha,Ameer Y, AU - Izadi,Hamid, AU - Stark,Ken D, Y1 - 2009/09/09/ PY - 2009/06/08/received PY - 2009/07/21/revised PY - 2009/07/24/accepted PY - 2009/9/12/entrez PY - 2009/9/12/pubmed PY - 2010/3/24/medline SP - 417 EP - 23 JF - Prostaglandins, leukotrienes, and essential fatty acids JO - Prostaglandins Leukot. Essent. Fatty Acids VL - 81 IS - 5-6 N2 - Ultrasound may reduce lipid extraction times and increase analysis throughput of food materials. Ground flaxseed (25mg aliquots) were extracted in quadruplicate in 2:1 (v:v) chloroform:methanol, 3:2 hexane:isopropanol, 1:1 diethyl:petroleum ether or hexane with exposure to sonication at low frequencies of 20kHz with a 600W ultrasonic processor. Power was automatically varied to maintain constant amplitudes of 20%, 60% and 100% of 240microm for sonication exposures for 5, 10 and 20min, respectively. Total lipid dry weights and quantitative and qualitative fatty acids were determined. Results were compared to a standard 24-h, Folch-based, 2:1 chloroform:methanol extraction. Longer time exposures and higher sonication amplitudes were associated with increases in lipid recoveries. In particular, ultrasound-assisted extraction in 3:2 hexane:isopropanol for only 10min resulted in lipid and fatty acid recoveries similar to the 24-h standard method. Comprehensive testing on a variety of sample matrices and food products is required, but lipid extraction by ultrasound has potential to reduce sample processing time. SN - 1532-2823 UR - https://www.unboundmedicine.com/medline/citation/19744844/The_application_of_ultrasound_energy_to_increase_lipid_extraction_throughput_of_solid_matrix_samples__flaxseed__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952-3278(09)00129-X DB - PRIME DP - Unbound Medicine ER -