Tags

Type your tag names separated by a space and hit enter

Swim stress enhances nociceptin/orphanin FQ-induced inhibition of rat dorsal raphe nucleus activity in vivo and in vitro: role of corticotropin releasing factor.
Neuropharmacology. 2010 Feb; 58(2):457-64.N

Abstract

The effects of nociceptin/orphanin FQ on putative serotonin (5HT) neurons of the dorsal raphe nucleus (DRN), known to modulate the behavioral responses to stress, were investigated in vivo and in vitro. In DRN slices from unstressed rats, nociceptin/orphanin FQ concentration-dependently inhibited the firing rate of putative 5HT neurons (EC(50) = 21.6 +/- 1.21 nM) and the selective NOP receptor antagonist UFP-101 shifted the concentration-response curve to the right (estimated pA(2) 6.86). Nociceptin/orphanin FQ potency was enhanced in slices prepared from rats previously subjected to a 15 min swim stress (EC(50) = 1.98 +/- 0.11 nM). Swim stress did not change the number or affinity of NOP receptors in DRN. Stress-elicited potentiation involved corticotropin-releasing factor (CRF)(1) receptors, GABA signaling and protein synthesis, being attenuated by pre-treatment with antalarmin (20 mg/kg, i.p.), diazepam (2.4 mg/kg, i.p.) and cycloheximide (2.5 mg/kg, i.p.), respectively. In anesthetized unstressed rats, locally applied nociceptin/orphanin FQ (0.03 and 0.1 ng/30 nl) inhibited the firing rate of DRN neurons (to 80 +/- 7 and 54 +/- 10% of baseline, respectively). Nociceptin/orphanin FQ inhibition was potentiated both 24 h after swim stress and 1 h after CRF (30 ng/30 nl intra-DRN). Stress-induced potentiation was prevented by the selective CRF(1) receptor antagonist, NBI 30755 (20 mg/kg, i.p.). In contrast, the inhibitory response of DRN neurons to the 5HT(1A) agonist, 8OH-DPAT (1 microg/1 microl, intra-DRN) was not potentiated by swim stress, ruling out a non-specific enhanced permeability of GIRK channel. Together, these findings suggest that CRF and the nociceptin/orphanin FQ/NOP system interact in the DRN during stress to control 5HT transmission; this may play a role in stress-related neuropsychopathologies.

Authors+Show Affiliations

Department of Clinical and Experimental Medicine, Section Pharmacology, University of Ferrara, via Fossato di Mortara 17-19, 44100-Ferrara, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19747494

Citation

Nazzaro, Cristiano, et al. "Swim Stress Enhances Nociceptin/orphanin FQ-induced Inhibition of Rat Dorsal Raphe Nucleus Activity in Vivo and in Vitro: Role of Corticotropin Releasing Factor." Neuropharmacology, vol. 58, no. 2, 2010, pp. 457-64.
Nazzaro C, Barbieri M, Varani K, et al. Swim stress enhances nociceptin/orphanin FQ-induced inhibition of rat dorsal raphe nucleus activity in vivo and in vitro: role of corticotropin releasing factor. Neuropharmacology. 2010;58(2):457-64.
Nazzaro, C., Barbieri, M., Varani, K., Beani, L., Valentino, R. J., & Siniscalchi, A. (2010). Swim stress enhances nociceptin/orphanin FQ-induced inhibition of rat dorsal raphe nucleus activity in vivo and in vitro: role of corticotropin releasing factor. Neuropharmacology, 58(2), 457-64. https://doi.org/10.1016/j.neuropharm.2009.09.004
Nazzaro C, et al. Swim Stress Enhances Nociceptin/orphanin FQ-induced Inhibition of Rat Dorsal Raphe Nucleus Activity in Vivo and in Vitro: Role of Corticotropin Releasing Factor. Neuropharmacology. 2010;58(2):457-64. PubMed PMID: 19747494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Swim stress enhances nociceptin/orphanin FQ-induced inhibition of rat dorsal raphe nucleus activity in vivo and in vitro: role of corticotropin releasing factor. AU - Nazzaro,Cristiano, AU - Barbieri,Mario, AU - Varani,Katia, AU - Beani,Lorenzo, AU - Valentino,Rita J, AU - Siniscalchi,Anna, Y1 - 2009/09/09/ PY - 2009/04/02/received PY - 2009/09/03/revised PY - 2009/09/03/accepted PY - 2009/9/15/entrez PY - 2009/9/15/pubmed PY - 2010/4/14/medline SP - 457 EP - 64 JF - Neuropharmacology JO - Neuropharmacology VL - 58 IS - 2 N2 - The effects of nociceptin/orphanin FQ on putative serotonin (5HT) neurons of the dorsal raphe nucleus (DRN), known to modulate the behavioral responses to stress, were investigated in vivo and in vitro. In DRN slices from unstressed rats, nociceptin/orphanin FQ concentration-dependently inhibited the firing rate of putative 5HT neurons (EC(50) = 21.6 +/- 1.21 nM) and the selective NOP receptor antagonist UFP-101 shifted the concentration-response curve to the right (estimated pA(2) 6.86). Nociceptin/orphanin FQ potency was enhanced in slices prepared from rats previously subjected to a 15 min swim stress (EC(50) = 1.98 +/- 0.11 nM). Swim stress did not change the number or affinity of NOP receptors in DRN. Stress-elicited potentiation involved corticotropin-releasing factor (CRF)(1) receptors, GABA signaling and protein synthesis, being attenuated by pre-treatment with antalarmin (20 mg/kg, i.p.), diazepam (2.4 mg/kg, i.p.) and cycloheximide (2.5 mg/kg, i.p.), respectively. In anesthetized unstressed rats, locally applied nociceptin/orphanin FQ (0.03 and 0.1 ng/30 nl) inhibited the firing rate of DRN neurons (to 80 +/- 7 and 54 +/- 10% of baseline, respectively). Nociceptin/orphanin FQ inhibition was potentiated both 24 h after swim stress and 1 h after CRF (30 ng/30 nl intra-DRN). Stress-induced potentiation was prevented by the selective CRF(1) receptor antagonist, NBI 30755 (20 mg/kg, i.p.). In contrast, the inhibitory response of DRN neurons to the 5HT(1A) agonist, 8OH-DPAT (1 microg/1 microl, intra-DRN) was not potentiated by swim stress, ruling out a non-specific enhanced permeability of GIRK channel. Together, these findings suggest that CRF and the nociceptin/orphanin FQ/NOP system interact in the DRN during stress to control 5HT transmission; this may play a role in stress-related neuropsychopathologies. SN - 1873-7064 UR - https://www.unboundmedicine.com/medline/citation/19747494/Swim_stress_enhances_nociceptin/orphanin_FQ_induced_inhibition_of_rat_dorsal_raphe_nucleus_activity_in_vivo_and_in_vitro:_role_of_corticotropin_releasing_factor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0028-3908(09)00309-8 DB - PRIME DP - Unbound Medicine ER -