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DNA aptamer-based QDs electrochemiluminescence biosensor for the detection of thrombin.
Biosens Bioelectron. 2009 Dec 15; 25(4):927-30.BB

Abstract

A novel biosensor for the detection of thrombin was developed by using QDs electrochemiluminescence (ECL) technique. The thiol-terminated aptamer with 15 nucleotides (probe I) was first immobilized on Au electrode, and then thrombin was imported to form the aptamer-thrombin bioaffinity complexes. Another 5'-biotin modified aptamer (29 nucleotides, probe II) was next hybridized with the combined thrombin to form a sandwich type structure. Streptavidin modified QDs (avidin-QDs) were bound to probe II via the biotin-avidin-system. The QDs ECL signal was responsive to the amount of probe II, which was indirect proportional to the combined thrombin. The ECL intensity of the biosensor increased with the increase of thrombin concentration in the range of 0-20 microgmL(-1). In addition, the biosensor exhibited the excellent selectivity responses and good stability toward the target analyte.

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Authors+Show Affiliations

Huang H
School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
Zhu JJ
No affiliation info available

MeSH

Aptamers, NucleotideBiosensing TechniquesElectrochemistryEquipment DesignEquipment Failure AnalysisImmunoassayLuminescent MeasurementsQuantum DotsThrombin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19747817

Citation

Huang, Haiping, and Jun-Jie Zhu. "DNA Aptamer-based QDs Electrochemiluminescence Biosensor for the Detection of Thrombin." Biosensors & Bioelectronics, vol. 25, no. 4, 2009, pp. 927-30.
Huang H, Zhu JJ. DNA aptamer-based QDs electrochemiluminescence biosensor for the detection of thrombin. Biosens Bioelectron. 2009;25(4):927-30.
Huang, H., & Zhu, J. J. (2009). DNA aptamer-based QDs electrochemiluminescence biosensor for the detection of thrombin. Biosensors & Bioelectronics, 25(4), 927-30. https://doi.org/10.1016/j.bios.2009.08.008
Huang H, Zhu JJ. DNA Aptamer-based QDs Electrochemiluminescence Biosensor for the Detection of Thrombin. Biosens Bioelectron. 2009 Dec 15;25(4):927-30. PubMed PMID: 19747817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA aptamer-based QDs electrochemiluminescence biosensor for the detection of thrombin. AU - Huang,Haiping, AU - Zhu,Jun-Jie, Y1 - 2009/08/12/ PY - 2009/05/07/received PY - 2009/07/17/revised PY - 2009/08/06/accepted PY - 2009/9/15/entrez PY - 2009/9/15/pubmed PY - 2010/1/14/medline SP - 927 EP - 30 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 25 IS - 4 N2 - A novel biosensor for the detection of thrombin was developed by using QDs electrochemiluminescence (ECL) technique. The thiol-terminated aptamer with 15 nucleotides (probe I) was first immobilized on Au electrode, and then thrombin was imported to form the aptamer-thrombin bioaffinity complexes. Another 5'-biotin modified aptamer (29 nucleotides, probe II) was next hybridized with the combined thrombin to form a sandwich type structure. Streptavidin modified QDs (avidin-QDs) were bound to probe II via the biotin-avidin-system. The QDs ECL signal was responsive to the amount of probe II, which was indirect proportional to the combined thrombin. The ECL intensity of the biosensor increased with the increase of thrombin concentration in the range of 0-20 microgmL(-1). In addition, the biosensor exhibited the excellent selectivity responses and good stability toward the target analyte. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19747817/DNA_aptamer_based_QDs_electrochemiluminescence_biosensor_for_the_detection_of_thrombin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(09)00431-X DB - PRIME DP - Unbound Medicine ER -