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Direct characterization of aqueous extract of Hibiscus sabdariffa using HPLC with diode array detection coupled to ESI and ion trap MS.
J Sep Sci. 2009 Oct; 32(20):3441-8.JS

Abstract

The phenolic fraction and other polar compounds of the Hibiscus sabdariffa were separated and identified by HPLC with diode array detection coupled to electrospray TOF and IT tandem MS (DAD-HPLC-ESI-TOF-MS and IT-MS). The H. sabdariffa aqueous extract was filtered and directly injected into the LC system. The analysis of the compounds was carried out by RP HPLC coupled to DAD and TOF-MS in order to obtain molecular formula and exact mass. Posterior analyses with IT-MS were performed and the fragmentation pattern and confirmation of the structures were achieved. The H. sabdariffa samples were successfully analyzed in positive and negative ionization modes with two optimized linear gradients. In positive mode, the two most representative anthocyanins and other compounds were identified whereas the phenolic fraction, hydroxycitric acid and its lactone were identified using the negative ionization mode.

Authors+Show Affiliations

Faculty of Sciences, Department of Analytical Chemistry, University of Granada, Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19750503

Citation

Rodríguez-Medina, Inmaculada C., et al. "Direct Characterization of Aqueous Extract of Hibiscus Sabdariffa Using HPLC With Diode Array Detection Coupled to ESI and Ion Trap MS." Journal of Separation Science, vol. 32, no. 20, 2009, pp. 3441-8.
Rodríguez-Medina IC, Beltrán-Debón R, Molina VM, et al. Direct characterization of aqueous extract of Hibiscus sabdariffa using HPLC with diode array detection coupled to ESI and ion trap MS. J Sep Sci. 2009;32(20):3441-8.
Rodríguez-Medina, I. C., Beltrán-Debón, R., Molina, V. M., Alonso-Villaverde, C., Joven, J., Menéndez, J. A., Segura-Carretero, A., & Fernández-Gutiérrez, A. (2009). Direct characterization of aqueous extract of Hibiscus sabdariffa using HPLC with diode array detection coupled to ESI and ion trap MS. Journal of Separation Science, 32(20), 3441-8. https://doi.org/10.1002/jssc.200900298
Rodríguez-Medina IC, et al. Direct Characterization of Aqueous Extract of Hibiscus Sabdariffa Using HPLC With Diode Array Detection Coupled to ESI and Ion Trap MS. J Sep Sci. 2009;32(20):3441-8. PubMed PMID: 19750503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct characterization of aqueous extract of Hibiscus sabdariffa using HPLC with diode array detection coupled to ESI and ion trap MS. AU - Rodríguez-Medina,Inmaculada C, AU - Beltrán-Debón,Raúl, AU - Molina,Vicente Micol, AU - Alonso-Villaverde,Carlos, AU - Joven,Jorge, AU - Menéndez,Javier A, AU - Segura-Carretero,Antonio, AU - Fernández-Gutiérrez,Alberto, PY - 2009/9/15/entrez PY - 2009/9/15/pubmed PY - 2010/1/6/medline SP - 3441 EP - 8 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 20 N2 - The phenolic fraction and other polar compounds of the Hibiscus sabdariffa were separated and identified by HPLC with diode array detection coupled to electrospray TOF and IT tandem MS (DAD-HPLC-ESI-TOF-MS and IT-MS). The H. sabdariffa aqueous extract was filtered and directly injected into the LC system. The analysis of the compounds was carried out by RP HPLC coupled to DAD and TOF-MS in order to obtain molecular formula and exact mass. Posterior analyses with IT-MS were performed and the fragmentation pattern and confirmation of the structures were achieved. The H. sabdariffa samples were successfully analyzed in positive and negative ionization modes with two optimized linear gradients. In positive mode, the two most representative anthocyanins and other compounds were identified whereas the phenolic fraction, hydroxycitric acid and its lactone were identified using the negative ionization mode. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/19750503/Direct_characterization_of_aqueous_extract_of_Hibiscus_sabdariffa_using_HPLC_with_diode_array_detection_coupled_to_ESI_and_ion_trap_MS_ L2 - https://doi.org/10.1002/jssc.200900298 DB - PRIME DP - Unbound Medicine ER -