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SERS in salt wells.
Chemphyschem. 2009 Oct 19; 10(15):2670-3.C

Abstract

We report herein a simple, inexpensive fabrication methodology of salt microwells, and define the utility of the latter as nanoparticle containers for highly sensitive surface-enhanced Raman scattering (SERS) studies. AFM characterization of Ag and Au loaded salt microwells reveal the ability to contain favorable nanostructures such as nanoparticle dimers, which can significantly enhance the Raman intensity of molecules. By performing diffraction-limited confocal Raman microscopy on salt microwells, we show high sensitivity and fidelity in the detection of dyes, peptides, and proteins, as a proof of our concept. The SERS limit of detection (accumulation time of 1 s) for rhodamine B and TAT contained in salt mircowells is 10 pM and 1 nM, respectively. The Raman characterization measurements of salt microwells with three different laser lines (532 nm, 632.81 nm, 785 nm) reveal low background intensity and high signal-to-noise ratio upon nanoparticle loading, which makes them suitable for enhanced Raman detection. SERS mapping of these sub-femtoliter containers show spatial confinement of the relevant analyte to a few microns, which make them potential candidates for microscale bioreactors.

Authors+Show Affiliations

Birck Nanotechnology & Bindley Bioscience Center, Agricultural & Biological Engineering, Purdue University, 225 S. University Street, West Lafayette, IN 47906, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19750533

Citation

Kumar, G V Pavan, and Joseph Irudayaraj. "SERS in Salt Wells." Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry, vol. 10, no. 15, 2009, pp. 2670-3.
Kumar GV, Irudayaraj J. SERS in salt wells. Chemphyschem. 2009;10(15):2670-3.
Kumar, G. V., & Irudayaraj, J. (2009). SERS in salt wells. Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry, 10(15), 2670-3. https://doi.org/10.1002/cphc.200900634
Kumar GV, Irudayaraj J. SERS in Salt Wells. Chemphyschem. 2009 Oct 19;10(15):2670-3. PubMed PMID: 19750533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SERS in salt wells. AU - Kumar,G V Pavan, AU - Irudayaraj,Joseph, PY - 2009/9/15/entrez PY - 2009/9/15/pubmed PY - 2010/1/16/medline SP - 2670 EP - 3 JF - Chemphyschem : a European journal of chemical physics and physical chemistry JO - Chemphyschem VL - 10 IS - 15 N2 - We report herein a simple, inexpensive fabrication methodology of salt microwells, and define the utility of the latter as nanoparticle containers for highly sensitive surface-enhanced Raman scattering (SERS) studies. AFM characterization of Ag and Au loaded salt microwells reveal the ability to contain favorable nanostructures such as nanoparticle dimers, which can significantly enhance the Raman intensity of molecules. By performing diffraction-limited confocal Raman microscopy on salt microwells, we show high sensitivity and fidelity in the detection of dyes, peptides, and proteins, as a proof of our concept. The SERS limit of detection (accumulation time of 1 s) for rhodamine B and TAT contained in salt mircowells is 10 pM and 1 nM, respectively. The Raman characterization measurements of salt microwells with three different laser lines (532 nm, 632.81 nm, 785 nm) reveal low background intensity and high signal-to-noise ratio upon nanoparticle loading, which makes them suitable for enhanced Raman detection. SERS mapping of these sub-femtoliter containers show spatial confinement of the relevant analyte to a few microns, which make them potential candidates for microscale bioreactors. SN - 1439-7641 UR - https://www.unboundmedicine.com/medline/citation/19750533/SERS_in_salt_wells_ L2 - https://doi.org/10.1002/cphc.200900634 DB - PRIME DP - Unbound Medicine ER -