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Circulating IgA and IgE autoantibodies in antilaminin-332 mucous membrane pemphigoid.
Br J Dermatol. 2010 Mar; 162(3):513-7.BJ

Abstract

BACKGROUND

Antilaminin-332 mucous membrane pemphigoid (MMP) is a chronic autoimmune bullous disease that is often associated with internal malignancy. IgG autoantibodies against laminin-332 in patients with MMP are well documented; however, IgA and IgE autoantibodies against laminin-332 have not yet been described.

OBJECTIVES

To characterize IgA and IgE autoantibodies binding to laminin-332 in sera from patients with antilaminin-332 MMP.

METHODS

Sera and skin samples from four patients who met the following criteria were used: (i) subepidermal blistering lesions present on the mucous membranes; (ii) in vivo deposition of IgG along the epidermal basement membrane zone of sampled skin; (iii) circulating IgG antibasement membrane zone antibodies that react with the dermal side of salt-split normal human skin; and (iv) circulating IgG autoantibodies that do not show positivity against type VII collagen or 200-kDa protein (p200 antigen) in immunoblot analysis using dermal extracts. Circulating IgG/IgA/IgE class autoantibodies against laminin-332 were determined by immunoblotting.

RESULTS

Circulating IgG autoantibodies against the gamma2, alpha3/gamma2, alpha3 and alpha3/beta3/gamma2 subunits of laminin-332 were demonstrated in sera from four patients, respectively. Serum from one of the four patients showed IgA reactivity with the alpha3/beta3/gamma2 subunits of laminin-332. Serum from one of the four patients showed IgE reactivity with the gamma2 subunit of laminin-332. The control sera failed to display IgG/IgA/IgE reactivity to laminin-332.

CONCLUSIONS

In addition to IgG autoantibodies, circulating IgA and IgE autoantibodies against laminin-332 are detectable in a subset of patients with antilaminin-332 MMP.

Authors+Show Affiliations

Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan. natsuga@med.hokudai.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

19751242

Citation

Natsuga, K, et al. "Circulating IgA and IgE Autoantibodies in Antilaminin-332 Mucous Membrane Pemphigoid." The British Journal of Dermatology, vol. 162, no. 3, 2010, pp. 513-7.
Natsuga K, Nishie W, Shinkuma S, et al. Circulating IgA and IgE autoantibodies in antilaminin-332 mucous membrane pemphigoid. Br J Dermatol. 2010;162(3):513-7.
Natsuga, K., Nishie, W., Shinkuma, S., Moriuchi, R., Shibata, M., Nishimura, M., Hashimoto, T., & Shimizu, H. (2010). Circulating IgA and IgE autoantibodies in antilaminin-332 mucous membrane pemphigoid. The British Journal of Dermatology, 162(3), 513-7. https://doi.org/10.1111/j.1365-2133.2009.09508.x
Natsuga K, et al. Circulating IgA and IgE Autoantibodies in Antilaminin-332 Mucous Membrane Pemphigoid. Br J Dermatol. 2010;162(3):513-7. PubMed PMID: 19751242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circulating IgA and IgE autoantibodies in antilaminin-332 mucous membrane pemphigoid. AU - Natsuga,K, AU - Nishie,W, AU - Shinkuma,S, AU - Moriuchi,R, AU - Shibata,M, AU - Nishimura,M, AU - Hashimoto,T, AU - Shimizu,H, Y1 - 2009/09/14/ PY - 2009/9/16/entrez PY - 2009/9/16/pubmed PY - 2010/9/21/medline SP - 513 EP - 7 JF - The British journal of dermatology JO - Br J Dermatol VL - 162 IS - 3 N2 - BACKGROUND: Antilaminin-332 mucous membrane pemphigoid (MMP) is a chronic autoimmune bullous disease that is often associated with internal malignancy. IgG autoantibodies against laminin-332 in patients with MMP are well documented; however, IgA and IgE autoantibodies against laminin-332 have not yet been described. OBJECTIVES: To characterize IgA and IgE autoantibodies binding to laminin-332 in sera from patients with antilaminin-332 MMP. METHODS: Sera and skin samples from four patients who met the following criteria were used: (i) subepidermal blistering lesions present on the mucous membranes; (ii) in vivo deposition of IgG along the epidermal basement membrane zone of sampled skin; (iii) circulating IgG antibasement membrane zone antibodies that react with the dermal side of salt-split normal human skin; and (iv) circulating IgG autoantibodies that do not show positivity against type VII collagen or 200-kDa protein (p200 antigen) in immunoblot analysis using dermal extracts. Circulating IgG/IgA/IgE class autoantibodies against laminin-332 were determined by immunoblotting. RESULTS: Circulating IgG autoantibodies against the gamma2, alpha3/gamma2, alpha3 and alpha3/beta3/gamma2 subunits of laminin-332 were demonstrated in sera from four patients, respectively. Serum from one of the four patients showed IgA reactivity with the alpha3/beta3/gamma2 subunits of laminin-332. Serum from one of the four patients showed IgE reactivity with the gamma2 subunit of laminin-332. The control sera failed to display IgG/IgA/IgE reactivity to laminin-332. CONCLUSIONS: In addition to IgG autoantibodies, circulating IgA and IgE autoantibodies against laminin-332 are detectable in a subset of patients with antilaminin-332 MMP. SN - 1365-2133 UR - https://www.unboundmedicine.com/medline/citation/19751242/Circulating_IgA_and_IgE_autoantibodies_in_antilaminin_332_mucous_membrane_pemphigoid_ L2 - https://doi.org/10.1111/j.1365-2133.2009.09508.x DB - PRIME DP - Unbound Medicine ER -