Tags

Type your tag names separated by a space and hit enter

Experimental ocular tuberculosis in guinea pigs.
Arch Ophthalmol. 2009 Sep; 127(9):1162-6.AO

Abstract

OBJECTIVE

To develop an animal model of intraocular tuberculosis (TB) with features of pulmonary TB and extrapulmonary dissemination to the eye.

METHODS

Hartley strain guinea pigs were infected via an aerosol route with virulent Mycobacterium tuberculosis. One group of guinea pigs was infected with a relatively low bacterial inoculum and received no treatment. A second group of guinea pigs received high-dose infection and were treated with the first-line anti-TB drugs isoniazid, rifampin, and pyrazinamide. Development of ocular TB lesions was documented by histological analysis, acid-fast staining, and real-time polymerase chain reaction for M tuberculosis DNA.

RESULTS

Untreated guinea pigs developed pulmonary and extrapulmonary TB. Ocular TB, primarily involving the uvea, developed in 5 of 12 eyes (42%). Uveal granulomatous lesions showed the presence of acid-fast organisms and M tuberculosis DNA. In treated animals, none of the 8 eyes examined revealed the presence of acid-fast organisms; however, there was mild nongranulomatous uveitis in 4 eyes.

CONCLUSIONS

Mycobacterium tuberculosis delivered via aerosol to guinea pigs results in extrapulmonary dissemination to the eye. Of significance, intraocular changes in this model include granulomatous inflammation and the presence of acid-fast organisms, as seen in human cases of ocular TB. Clinical Relevance The guinea pig model may provide greater insight into the pathogenesis of intraocular TB and assist in the development of novel modalities to treat this global infectious disease.

Authors+Show Affiliations

Doheny Eye Institute and Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, California, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19752425

Citation

Rao, Narsing A., et al. "Experimental Ocular Tuberculosis in Guinea Pigs." Archives of Ophthalmology (Chicago, Ill. : 1960), vol. 127, no. 9, 2009, pp. 1162-6.
Rao NA, Albini TA, Kumaradas M, et al. Experimental ocular tuberculosis in guinea pigs. Arch Ophthalmol. 2009;127(9):1162-6.
Rao, N. A., Albini, T. A., Kumaradas, M., Pinn, M. L., Fraig, M. M., & Karakousis, P. C. (2009). Experimental ocular tuberculosis in guinea pigs. Archives of Ophthalmology (Chicago, Ill. : 1960), 127(9), 1162-6. https://doi.org/10.1001/archophthalmol.2009.220
Rao NA, et al. Experimental Ocular Tuberculosis in Guinea Pigs. Arch Ophthalmol. 2009;127(9):1162-6. PubMed PMID: 19752425.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Experimental ocular tuberculosis in guinea pigs. AU - Rao,Narsing A, AU - Albini,Thomas A, AU - Kumaradas,Mirnalini, AU - Pinn,Michael L, AU - Fraig,Mostafa M, AU - Karakousis,Petros C, PY - 2009/9/16/entrez PY - 2009/9/16/pubmed PY - 2009/9/29/medline SP - 1162 EP - 6 JF - Archives of ophthalmology (Chicago, Ill. : 1960) JO - Arch. Ophthalmol. VL - 127 IS - 9 N2 - OBJECTIVE: To develop an animal model of intraocular tuberculosis (TB) with features of pulmonary TB and extrapulmonary dissemination to the eye. METHODS: Hartley strain guinea pigs were infected via an aerosol route with virulent Mycobacterium tuberculosis. One group of guinea pigs was infected with a relatively low bacterial inoculum and received no treatment. A second group of guinea pigs received high-dose infection and were treated with the first-line anti-TB drugs isoniazid, rifampin, and pyrazinamide. Development of ocular TB lesions was documented by histological analysis, acid-fast staining, and real-time polymerase chain reaction for M tuberculosis DNA. RESULTS: Untreated guinea pigs developed pulmonary and extrapulmonary TB. Ocular TB, primarily involving the uvea, developed in 5 of 12 eyes (42%). Uveal granulomatous lesions showed the presence of acid-fast organisms and M tuberculosis DNA. In treated animals, none of the 8 eyes examined revealed the presence of acid-fast organisms; however, there was mild nongranulomatous uveitis in 4 eyes. CONCLUSIONS: Mycobacterium tuberculosis delivered via aerosol to guinea pigs results in extrapulmonary dissemination to the eye. Of significance, intraocular changes in this model include granulomatous inflammation and the presence of acid-fast organisms, as seen in human cases of ocular TB. Clinical Relevance The guinea pig model may provide greater insight into the pathogenesis of intraocular TB and assist in the development of novel modalities to treat this global infectious disease. SN - 1538-3601 UR - https://www.unboundmedicine.com/medline/citation/19752425/Experimental_ocular_tuberculosis_in_guinea_pigs_ L2 - https://jamanetwork.com/journals/jamaophthalmology/fullarticle/10.1001/archophthalmol.2009.220 DB - PRIME DP - Unbound Medicine ER -