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Direct electron transfer and electrocatalysis of hemoglobin in ZnO coated multiwalled carbon nanotubes and Nafion composite matrix.
Bioelectrochemistry. 2010 Jun; 78(2):106-12.B

Abstract

ZnO coated multiwalled carbon nanotubes (ZnO-MWCNTs) nano-composite was synthesized by the hydrothermal method and expected to offer a promising template for biosensor fabrication due to satisfying biocompatibility and improved properties. The ZnO-MWCNTs nano-composite was mixed with Nafion solution to form a composite matrix for the fabrication of hemoglobin (Hb) biosensor. This composite matrix combined the advantages of inorganic composite (ZnO-MWCNTs) and organic polymer (Nafion) and could promote the direct electron transfer of Hb. The Hb/ZnO-MWCNTs/Nafion film exhibited a pair of well-defined, quasi-reversible redox peaks with a formal potential of -0.353V (vs. SCE), characteristic of heme redox couple (Fe(III)/Fe(II)) of Hb. Hb retained its near-native conformation in the composite film. The immobilized Hb showed fast and excellent electrocatalytic activity to H(2)O(2) with a linear range from 2x10(-7) to 1.2x10(-5)M, and the detection limit was 8.4x10(-8)M. The sensitivity and apparent Michaelis-Menten constant were 1.31A/(M x cm(2)) and 82.8microM, respectively, which indicated that Hb had a high affinity to H(2)O(2). This biosensor also showed excellent electrocatalytic activity towards trichloroacetic acid.

Authors+Show Affiliations

College of Science, Nanjing University of Technology, Nanjing 210009, PR China.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19758846

Citation

Ma, Wei, and Danbi Tian. "Direct Electron Transfer and Electrocatalysis of Hemoglobin in ZnO Coated Multiwalled Carbon Nanotubes and Nafion Composite Matrix." Bioelectrochemistry (Amsterdam, Netherlands), vol. 78, no. 2, 2010, pp. 106-12.
Ma W, Tian D. Direct electron transfer and electrocatalysis of hemoglobin in ZnO coated multiwalled carbon nanotubes and Nafion composite matrix. Bioelectrochemistry. 2010;78(2):106-12.
Ma, W., & Tian, D. (2010). Direct electron transfer and electrocatalysis of hemoglobin in ZnO coated multiwalled carbon nanotubes and Nafion composite matrix. Bioelectrochemistry (Amsterdam, Netherlands), 78(2), 106-12. https://doi.org/10.1016/j.bioelechem.2009.08.002
Ma W, Tian D. Direct Electron Transfer and Electrocatalysis of Hemoglobin in ZnO Coated Multiwalled Carbon Nanotubes and Nafion Composite Matrix. Bioelectrochemistry. 2010;78(2):106-12. PubMed PMID: 19758846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct electron transfer and electrocatalysis of hemoglobin in ZnO coated multiwalled carbon nanotubes and Nafion composite matrix. AU - Ma,Wei, AU - Tian,Danbi, Y1 - 2009/08/17/ PY - 2008/10/09/received PY - 2009/08/05/revised PY - 2009/08/09/accepted PY - 2009/9/18/entrez PY - 2009/9/18/pubmed PY - 2010/6/24/medline SP - 106 EP - 12 JF - Bioelectrochemistry (Amsterdam, Netherlands) JO - Bioelectrochemistry VL - 78 IS - 2 N2 - ZnO coated multiwalled carbon nanotubes (ZnO-MWCNTs) nano-composite was synthesized by the hydrothermal method and expected to offer a promising template for biosensor fabrication due to satisfying biocompatibility and improved properties. The ZnO-MWCNTs nano-composite was mixed with Nafion solution to form a composite matrix for the fabrication of hemoglobin (Hb) biosensor. This composite matrix combined the advantages of inorganic composite (ZnO-MWCNTs) and organic polymer (Nafion) and could promote the direct electron transfer of Hb. The Hb/ZnO-MWCNTs/Nafion film exhibited a pair of well-defined, quasi-reversible redox peaks with a formal potential of -0.353V (vs. SCE), characteristic of heme redox couple (Fe(III)/Fe(II)) of Hb. Hb retained its near-native conformation in the composite film. The immobilized Hb showed fast and excellent electrocatalytic activity to H(2)O(2) with a linear range from 2x10(-7) to 1.2x10(-5)M, and the detection limit was 8.4x10(-8)M. The sensitivity and apparent Michaelis-Menten constant were 1.31A/(M x cm(2)) and 82.8microM, respectively, which indicated that Hb had a high affinity to H(2)O(2). This biosensor also showed excellent electrocatalytic activity towards trichloroacetic acid. SN - 1878-562X UR - https://www.unboundmedicine.com/medline/citation/19758846/Direct_electron_transfer_and_electrocatalysis_of_hemoglobin_in_ZnO_coated_multiwalled_carbon_nanotubes_and_Nafion_composite_matrix_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5394(09)00162-5 DB - PRIME DP - Unbound Medicine ER -