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Screen and genetic assessment of tea germplasms with elevated methylated catechin, (-)-epigallocatechin-3-O-(3-O-methyl)gallate.
J Agric Food Chem. 2009 Oct 14; 57(19):8906-12.JA

Abstract

The aim of this research was to identify tea varieties containing high levels of catechin methyl ester that could be used as important sources for genetic breeding and in the production of high quality tea. We examined 113 species that have been preserved in the Taitung Branch of the Tea Research and Extension Station of Taiwan (TTES). The average level of (-)-epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3''Me) was 0.45% for all varieties screened. Among them, 16 varieties with higher EGCG3''Me content (>0.8%) were considered good candidates for manufacturing partially fermented tea. Analysis of these tea varieties revealed that the EGCG3''Me content in leaves did not correlate with the caffeine content. Genetic assessments revealed that the lengths of their internal transcribed spacers (ITS) were in the range of 638-670 bp and that the sequence identities were in the range of 0.690-1. Two major groups were constructed by phylogenetic analysis, I and II, with a genetic distance of 0.08 based on the ITS1-5.8S-ITS2 sequences between the ribosomal genes. Our results provide genetic information about tea varieties with elevated EGCG3''Me content and indicate the need for a comprehensive genetic assessment of tea germplasms preserved in the TTES to better serve the future of tea breeding.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Lee SC
Department of BioIndustry Technology, Da-Yeh University, Changhua 51591, Taiwan.
Yan RH
No affiliation info available
Cheng HY
No affiliation info available
Wu SS
No affiliation info available
Liu SY
No affiliation info available

MeSH

BreedingCaffeineCamellia sinensisCatechinMethylationPhylogenyPlant LeavesSpecies SpecificityTaiwan

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19761185

Citation

Lee, Shih-Chieh, et al. "Screen and Genetic Assessment of Tea Germplasms With Elevated Methylated Catechin, (-)-epigallocatechin-3-O-(3-O-methyl)gallate." Journal of Agricultural and Food Chemistry, vol. 57, no. 19, 2009, pp. 8906-12.
Lee SC, Yan RH, Cheng HY, et al. Screen and genetic assessment of tea germplasms with elevated methylated catechin, (-)-epigallocatechin-3-O-(3-O-methyl)gallate. J Agric Food Chem. 2009;57(19):8906-12.
Lee, S. C., Yan, R. H., Cheng, H. Y., Wu, S. S., & Liu, S. Y. (2009). Screen and genetic assessment of tea germplasms with elevated methylated catechin, (-)-epigallocatechin-3-O-(3-O-methyl)gallate. Journal of Agricultural and Food Chemistry, 57(19), 8906-12. https://doi.org/10.1021/jf901733c
Lee SC, et al. Screen and Genetic Assessment of Tea Germplasms With Elevated Methylated Catechin, (-)-epigallocatechin-3-O-(3-O-methyl)gallate. J Agric Food Chem. 2009 Oct 14;57(19):8906-12. PubMed PMID: 19761185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screen and genetic assessment of tea germplasms with elevated methylated catechin, (-)-epigallocatechin-3-O-(3-O-methyl)gallate. AU - Lee,Shih-Chieh, AU - Yan,Rui-Hong, AU - Cheng,Hun-Yuan, AU - Wu,Sang-Shung, AU - Liu,Shu-Ying, PY - 2009/9/19/entrez PY - 2009/9/19/pubmed PY - 2010/1/29/medline SP - 8906 EP - 12 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 19 N2 - The aim of this research was to identify tea varieties containing high levels of catechin methyl ester that could be used as important sources for genetic breeding and in the production of high quality tea. We examined 113 species that have been preserved in the Taitung Branch of the Tea Research and Extension Station of Taiwan (TTES). The average level of (-)-epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3''Me) was 0.45% for all varieties screened. Among them, 16 varieties with higher EGCG3''Me content (>0.8%) were considered good candidates for manufacturing partially fermented tea. Analysis of these tea varieties revealed that the EGCG3''Me content in leaves did not correlate with the caffeine content. Genetic assessments revealed that the lengths of their internal transcribed spacers (ITS) were in the range of 638-670 bp and that the sequence identities were in the range of 0.690-1. Two major groups were constructed by phylogenetic analysis, I and II, with a genetic distance of 0.08 based on the ITS1-5.8S-ITS2 sequences between the ribosomal genes. Our results provide genetic information about tea varieties with elevated EGCG3''Me content and indicate the need for a comprehensive genetic assessment of tea germplasms preserved in the TTES to better serve the future of tea breeding. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19761185/Screen_and_genetic_assessment_of_tea_germplasms_with_elevated_methylated_catechin_____epigallocatechin_3_O__3_O_methyl_gallate_ L2 - https://doi.org/10.1021/jf901733c DB - PRIME DP - Unbound Medicine ER -