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Validation of an ultra high pressure liquid chromatographic method for the determination of biologically active amines in food.
J Chromatogr A. 2009 Nov 06; 1216(45):7715-20.JC

Abstract

Biologically active amines include the so called biogenic amines, such as histamine, tyramine and cadaverine, and polyamines such as spermidine and spermine. Ultra high pressure liquid chromatography (UHPLC) is a new generation of separation techniques that takes full advantage of chromatographic principles to increase speed flow which drastically reduce analysis time. The aim of the present work was to validate a rapid method of UHPLC to detect the presence of biogenic amines and polyamines in food. Different food matrixes (wine, fish, cheese, and dry fermented sausage) were used in order to test the versatility of the method. The UHPLC method described in this article has been demonstrated as a reliable procedure to determine 12 biogenic amines and polyamines in less than 7min of chromatographic elution. The method provides a satisfactory linearity and chromatographic sensitivity with a detection limit lower than 0.2mg/L and a determination limit falling below 0.3mg/L for all amines. The precision, in terms of relative standard deviation, was lower than 5% and the accuracy, as mean recovery, was between 93% and 98%, depending on the food matrix.

Authors+Show Affiliations

Department of Nutrition and Food Science, XaRTA (Catalonian Reference Network on Food Technology) - INSA (Nutrition and Food Safety Research Institute), UB, Av. Joan XXIII s/n, E-08028, Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19762030

Citation

Latorre-Moratalla, M L., et al. "Validation of an Ultra High Pressure Liquid Chromatographic Method for the Determination of Biologically Active Amines in Food." Journal of Chromatography. A, vol. 1216, no. 45, 2009, pp. 7715-20.
Latorre-Moratalla ML, Bosch-Fusté J, Lavizzari T, et al. Validation of an ultra high pressure liquid chromatographic method for the determination of biologically active amines in food. J Chromatogr A. 2009;1216(45):7715-20.
Latorre-Moratalla, M. L., Bosch-Fusté, J., Lavizzari, T., Bover-Cid, S., Veciana-Nogués, M. T., & Vidal-Carou, M. C. (2009). Validation of an ultra high pressure liquid chromatographic method for the determination of biologically active amines in food. Journal of Chromatography. A, 1216(45), 7715-20. https://doi.org/10.1016/j.chroma.2009.08.072
Latorre-Moratalla ML, et al. Validation of an Ultra High Pressure Liquid Chromatographic Method for the Determination of Biologically Active Amines in Food. J Chromatogr A. 2009 Nov 6;1216(45):7715-20. PubMed PMID: 19762030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validation of an ultra high pressure liquid chromatographic method for the determination of biologically active amines in food. AU - Latorre-Moratalla,M L, AU - Bosch-Fusté,J, AU - Lavizzari,T, AU - Bover-Cid,S, AU - Veciana-Nogués,M T, AU - Vidal-Carou,M C, Y1 - 2009/09/01/ PY - 2009/04/22/received PY - 2009/08/23/revised PY - 2009/08/27/accepted PY - 2009/9/19/entrez PY - 2009/9/19/pubmed PY - 2009/12/16/medline SP - 7715 EP - 20 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 45 N2 - Biologically active amines include the so called biogenic amines, such as histamine, tyramine and cadaverine, and polyamines such as spermidine and spermine. Ultra high pressure liquid chromatography (UHPLC) is a new generation of separation techniques that takes full advantage of chromatographic principles to increase speed flow which drastically reduce analysis time. The aim of the present work was to validate a rapid method of UHPLC to detect the presence of biogenic amines and polyamines in food. Different food matrixes (wine, fish, cheese, and dry fermented sausage) were used in order to test the versatility of the method. The UHPLC method described in this article has been demonstrated as a reliable procedure to determine 12 biogenic amines and polyamines in less than 7min of chromatographic elution. The method provides a satisfactory linearity and chromatographic sensitivity with a detection limit lower than 0.2mg/L and a determination limit falling below 0.3mg/L for all amines. The precision, in terms of relative standard deviation, was lower than 5% and the accuracy, as mean recovery, was between 93% and 98%, depending on the food matrix. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19762030/Validation_of_an_ultra_high_pressure_liquid_chromatographic_method_for_the_determination_of_biologically_active_amines_in_food_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)01305-3 DB - PRIME DP - Unbound Medicine ER -