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Pomegranate juice polyphenols increase recombinant paraoxonase-1 binding to high-density lipoprotein: studies in vitro and in diabetic patients.
Nutrition. 2010 Apr; 26(4):359-66.N

Abstract

OBJECTIVE

The high-density lipoprotein (HDL)-associated paraoxonase-1 (PON1)/free PON1 ratio is lower in diabetic patients in comparison with healthy controls. Because diabetes is associated with increased oxidative stress, we hypothesized that a labeled recombinant PON1 (rePON1) would detect differences in HDL capacity to bind PON1 under specific experimental conditions, such as oxidation, addition of polyphenols, or in vivo dosing of diabetic patients with polyphenols.

METHODS

In the present study we determined labeled rePON1 binding to HDL under various oxidative conditions by using polyacrylamide gel electrophoresis for the separation of free labeled rePON1 from HDL-bound labeled rePON1.

RESULTS

The HDL-rePON1/free rePON1 ratio gradually decreased as the extent of HDL oxidation increased, and the antioxidants vitamin E or pomegranate juice (PJ) inhibited the redistribution of rePON1. PJ or its purified polyphenols, punicalagin, gallic acid, or ellagic acid, increased rePON1 binding also to non-oxidized HDL. Further, rePON1 associated more efficiently with HDLs isolated from diabetic patients after PJ consumption versus HDLs isolated before PJ consumption.

CONCLUSIONS

We conclude that 1) oxidative stress impairs binding of fluorescein isothiocyanate-labeled rePON1 to HDL and 2) PJ polyphenols directly increase the HDL-rePON1 association beyond their antioxidative effect.

Authors+Show Affiliations

Rambam Medical Center, Rappaport Family Institute for Research in Medical Sciences and Technion Faculty of Medicine, Haifa, Israel. Fuhrman@tx.technion.ac.ilNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19762215

Citation

Fuhrman, Bianca, et al. "Pomegranate Juice Polyphenols Increase Recombinant Paraoxonase-1 Binding to High-density Lipoprotein: Studies in Vitro and in Diabetic Patients." Nutrition (Burbank, Los Angeles County, Calif.), vol. 26, no. 4, 2010, pp. 359-66.
Fuhrman B, Volkova N, Aviram M. Pomegranate juice polyphenols increase recombinant paraoxonase-1 binding to high-density lipoprotein: studies in vitro and in diabetic patients. Nutrition. 2010;26(4):359-66.
Fuhrman, B., Volkova, N., & Aviram, M. (2010). Pomegranate juice polyphenols increase recombinant paraoxonase-1 binding to high-density lipoprotein: studies in vitro and in diabetic patients. Nutrition (Burbank, Los Angeles County, Calif.), 26(4), 359-66. https://doi.org/10.1016/j.nut.2009.05.003
Fuhrman B, Volkova N, Aviram M. Pomegranate Juice Polyphenols Increase Recombinant Paraoxonase-1 Binding to High-density Lipoprotein: Studies in Vitro and in Diabetic Patients. Nutrition. 2010;26(4):359-66. PubMed PMID: 19762215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pomegranate juice polyphenols increase recombinant paraoxonase-1 binding to high-density lipoprotein: studies in vitro and in diabetic patients. AU - Fuhrman,Bianca, AU - Volkova,Nina, AU - Aviram,Michael, Y1 - 2009/09/17/ PY - 2009/02/22/received PY - 2009/05/04/revised PY - 2009/05/05/accepted PY - 2009/9/19/entrez PY - 2009/9/19/pubmed PY - 2010/5/28/medline SP - 359 EP - 66 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 26 IS - 4 N2 - OBJECTIVE: The high-density lipoprotein (HDL)-associated paraoxonase-1 (PON1)/free PON1 ratio is lower in diabetic patients in comparison with healthy controls. Because diabetes is associated with increased oxidative stress, we hypothesized that a labeled recombinant PON1 (rePON1) would detect differences in HDL capacity to bind PON1 under specific experimental conditions, such as oxidation, addition of polyphenols, or in vivo dosing of diabetic patients with polyphenols. METHODS: In the present study we determined labeled rePON1 binding to HDL under various oxidative conditions by using polyacrylamide gel electrophoresis for the separation of free labeled rePON1 from HDL-bound labeled rePON1. RESULTS: The HDL-rePON1/free rePON1 ratio gradually decreased as the extent of HDL oxidation increased, and the antioxidants vitamin E or pomegranate juice (PJ) inhibited the redistribution of rePON1. PJ or its purified polyphenols, punicalagin, gallic acid, or ellagic acid, increased rePON1 binding also to non-oxidized HDL. Further, rePON1 associated more efficiently with HDLs isolated from diabetic patients after PJ consumption versus HDLs isolated before PJ consumption. CONCLUSIONS: We conclude that 1) oxidative stress impairs binding of fluorescein isothiocyanate-labeled rePON1 to HDL and 2) PJ polyphenols directly increase the HDL-rePON1 association beyond their antioxidative effect. SN - 1873-1244 UR - https://www.unboundmedicine.com/medline/citation/19762215/Pomegranate_juice_polyphenols_increase_recombinant_paraoxonase_1_binding_to_high_density_lipoprotein:_studies_in_vitro_and_in_diabetic_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(09)00221-4 DB - PRIME DP - Unbound Medicine ER -