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Licking decreases phosphorylation of extracellular signal-regulated kinase in the dorsal horn of the spinal cord after a formalin test.
Anesth Analg. 2009 Oct; 109(4):1318-22.A&A

Abstract

BACKGROUND

Nociceptive behaviors might attenuate pain sensation. Phosphorylation of extracellular signal-regulated kinase (pERK) was recently reported to be induced by noxious stimuli in dorsal horn neurons. We investigated, in a formalin test, whether pERK of the dorsal horn is affected by licking.

METHODS

Twenty-four adult male rats were divided into four groups: control, formalin test, restricted control, and restricted formalin test. Ten percent formalin was injected subcutaneously into the left rear paw of the formalin test and restricted formalin test groups. The control and formalin test group rats were kept in a clear plastic chamber, whereas the restricted control and restricted formalin test group rats were kept in a modified-restraint, pipe-shaped chamber. All rats were killed after 25 min. Twelve sections of the lumbar spinal cord were processed for p-ERK immunohistochemistry using the avidin-biotin peroxidase method.

RESULTS

The number of p-ERK positive cells in the restricted formalin test group was significantly higher than in the other three groups in the ipsilateral-side superficial dorsal horn (P < 0.05). However, there was no significant difference between the formalin test group and the two control groups in pERK expression.

CONCLUSION

Licking decreased pERK of the spinal cord of the formalin test group. The findings suggested that licking attenuated the pain of the formalin test.

Authors+Show Affiliations

Department of Anesthesiology, Institute of Clinical Medicine, Graduate School of Comprehensive Human Sciences, Tsukuba University, Tsukuba-city, Ibaraki 305-8575, Japan. taekof@md.tsukuba.ac.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19762763

Citation

Fukuda, Taeko, et al. "Licking Decreases Phosphorylation of Extracellular Signal-regulated Kinase in the Dorsal Horn of the Spinal Cord After a Formalin Test." Anesthesia and Analgesia, vol. 109, no. 4, 2009, pp. 1318-22.
Fukuda T, Hisano S, Tanaka M. Licking decreases phosphorylation of extracellular signal-regulated kinase in the dorsal horn of the spinal cord after a formalin test. Anesth Analg. 2009;109(4):1318-22.
Fukuda, T., Hisano, S., & Tanaka, M. (2009). Licking decreases phosphorylation of extracellular signal-regulated kinase in the dorsal horn of the spinal cord after a formalin test. Anesthesia and Analgesia, 109(4), 1318-22. https://doi.org/10.1213/ane.0b013e3181b0fe05
Fukuda T, Hisano S, Tanaka M. Licking Decreases Phosphorylation of Extracellular Signal-regulated Kinase in the Dorsal Horn of the Spinal Cord After a Formalin Test. Anesth Analg. 2009;109(4):1318-22. PubMed PMID: 19762763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Licking decreases phosphorylation of extracellular signal-regulated kinase in the dorsal horn of the spinal cord after a formalin test. AU - Fukuda,Taeko, AU - Hisano,Setsuji, AU - Tanaka,Makoto, PY - 2009/9/19/entrez PY - 2009/9/19/pubmed PY - 2009/10/2/medline SP - 1318 EP - 22 JF - Anesthesia and analgesia JO - Anesth Analg VL - 109 IS - 4 N2 - BACKGROUND: Nociceptive behaviors might attenuate pain sensation. Phosphorylation of extracellular signal-regulated kinase (pERK) was recently reported to be induced by noxious stimuli in dorsal horn neurons. We investigated, in a formalin test, whether pERK of the dorsal horn is affected by licking. METHODS: Twenty-four adult male rats were divided into four groups: control, formalin test, restricted control, and restricted formalin test. Ten percent formalin was injected subcutaneously into the left rear paw of the formalin test and restricted formalin test groups. The control and formalin test group rats were kept in a clear plastic chamber, whereas the restricted control and restricted formalin test group rats were kept in a modified-restraint, pipe-shaped chamber. All rats were killed after 25 min. Twelve sections of the lumbar spinal cord were processed for p-ERK immunohistochemistry using the avidin-biotin peroxidase method. RESULTS: The number of p-ERK positive cells in the restricted formalin test group was significantly higher than in the other three groups in the ipsilateral-side superficial dorsal horn (P < 0.05). However, there was no significant difference between the formalin test group and the two control groups in pERK expression. CONCLUSION: Licking decreased pERK of the spinal cord of the formalin test group. The findings suggested that licking attenuated the pain of the formalin test. SN - 1526-7598 UR - https://www.unboundmedicine.com/medline/citation/19762763/Licking_decreases_phosphorylation_of_extracellular_signal_regulated_kinase_in_the_dorsal_horn_of_the_spinal_cord_after_a_formalin_test_ L2 - https://doi.org/10.1213/ane.0b013e3181b0fe05 DB - PRIME DP - Unbound Medicine ER -