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Evidence of field-evolved resistance to Cry1Ac-expressing Bt cotton in Helicoverpa armigera (Lepidoptera: Noctuidae) in northern China.
Pest Manag Sci. 2010 Feb; 66(2):155-61.PM

Abstract

BACKGROUND

Evolution of resistance threatens the continued success of transgenic crops expressing insecticidal proteins. One of the key factors for successful resistance management is the timely implementation of monitoring programmes to detect early changes of resistance allele frequency in field populations. F(1)/F(2) screen, dose-response bioassays and field survey were used to monitor resistance to the Cry1Ac-expressing cotton in a field population of Helicoverpa armigera (Hübner), the primary target of transgenic Bt cotton in China.

RESULTS

Field survey showed an increased trend of egg populations of H. armigera on Bt cotton in the Qiuxian area from 2003 to 2007. By using the F(2) screening procedure, the resistance allele frequency in the Qiuxian (Hebei, China) population of H. armigera collected during 2007 was estimated to be 0.075 (95% CI: 0.053-0.100), which was 12 times greater than that estimated 9 years ago. Dose-response bioassay with the field population collected from the same area showed a significant resistance level (11-fold) to Cry1Ac toxin compared to a laboratory susceptible strain.

CONCLUSION

This study documented a case of field-evolved resistance in H. armigera after several years of intensive planting of Bt cotton. Proactive tactics must be adopted to prevent further increase of resistance gene frequency in the Qiuxian region.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Liu F
Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Xu Z
No affiliation info available
Zhu YC
No affiliation info available
Huang F
No affiliation info available
Wang Y
No affiliation info available
Li H
No affiliation info available
Li H
No affiliation info available
Gao C
No affiliation info available
Zhou W
No affiliation info available
Shen J
No affiliation info available

MeSH

AnimalsBacillus thuringiensis ToxinsBacterial ProteinsChinaEndotoxinsFemaleGossypiumHemolysin ProteinsInsecticide ResistanceInsecticidesMaleMothsPest Control, BiologicalPlants, Genetically Modified

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19764057

Citation

Liu, Fengyi, et al. "Evidence of Field-evolved Resistance to Cry1Ac-expressing Bt Cotton in Helicoverpa Armigera (Lepidoptera: Noctuidae) in Northern China." Pest Management Science, vol. 66, no. 2, 2010, pp. 155-61.
Liu F, Xu Z, Zhu YC, et al. Evidence of field-evolved resistance to Cry1Ac-expressing Bt cotton in Helicoverpa armigera (Lepidoptera: Noctuidae) in northern China. Pest Manag Sci. 2010;66(2):155-61.
Liu, F., Xu, Z., Zhu, Y. C., Huang, F., Wang, Y., Li, H., Li, H., Gao, C., Zhou, W., & Shen, J. (2010). Evidence of field-evolved resistance to Cry1Ac-expressing Bt cotton in Helicoverpa armigera (Lepidoptera: Noctuidae) in northern China. Pest Management Science, 66(2), 155-61. https://doi.org/10.1002/ps.1849
Liu F, et al. Evidence of Field-evolved Resistance to Cry1Ac-expressing Bt Cotton in Helicoverpa Armigera (Lepidoptera: Noctuidae) in Northern China. Pest Manag Sci. 2010;66(2):155-61. PubMed PMID: 19764057.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of field-evolved resistance to Cry1Ac-expressing Bt cotton in Helicoverpa armigera (Lepidoptera: Noctuidae) in northern China. AU - Liu,Fengyi, AU - Xu,Zhiping, AU - Zhu,Yu Cheng, AU - Huang,Fangneng, AU - Wang,Yanhua, AU - Li,Huiling, AU - Li,Hua, AU - Gao,Congfen, AU - Zhou,Weijun, AU - Shen,Jinliang, PY - 2009/9/19/entrez PY - 2009/9/19/pubmed PY - 2010/3/6/medline SP - 155 EP - 61 JF - Pest management science JO - Pest Manag Sci VL - 66 IS - 2 N2 - BACKGROUND: Evolution of resistance threatens the continued success of transgenic crops expressing insecticidal proteins. One of the key factors for successful resistance management is the timely implementation of monitoring programmes to detect early changes of resistance allele frequency in field populations. F(1)/F(2) screen, dose-response bioassays and field survey were used to monitor resistance to the Cry1Ac-expressing cotton in a field population of Helicoverpa armigera (Hübner), the primary target of transgenic Bt cotton in China. RESULTS: Field survey showed an increased trend of egg populations of H. armigera on Bt cotton in the Qiuxian area from 2003 to 2007. By using the F(2) screening procedure, the resistance allele frequency in the Qiuxian (Hebei, China) population of H. armigera collected during 2007 was estimated to be 0.075 (95% CI: 0.053-0.100), which was 12 times greater than that estimated 9 years ago. Dose-response bioassay with the field population collected from the same area showed a significant resistance level (11-fold) to Cry1Ac toxin compared to a laboratory susceptible strain. CONCLUSION: This study documented a case of field-evolved resistance in H. armigera after several years of intensive planting of Bt cotton. Proactive tactics must be adopted to prevent further increase of resistance gene frequency in the Qiuxian region. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/19764057/Evidence_of_field_evolved_resistance_to_Cry1Ac_expressing_Bt_cotton_in_Helicoverpa_armigera__Lepidoptera:_Noctuidae__in_northern_China_ L2 - https://doi.org/10.1002/ps.1849 DB - PRIME DP - Unbound Medicine ER -