[HPLC fingerprint of the antiarrhythmic fraction of Valeriana officinalis].Zhong Yao Cai. 2009 Jun; 32(6):866-70.ZY
To establish HPLC fingerprints of the Antiarrhythmic fraction of Valeriana officinalis.
Agilent C18 (250 mm x 4.6 mm, 5 microm) column was used and the acetonitrile-water was chosen as the mobile phase in a gradient mode. The column temperature was 380 degrees C and the detection wavelength was 218 nm. The detection time was 70 min, and the flow rate was 1.0 mL/ min.
Fifteen characteristic peaks were indicated in HPLC fingerprints. The relative retention time and the ranges of relative areas of the common peaks were also determined.
This method is simple and accurate with a good reproducibility and provides a reference standard for the quality control of Valeriana officinalis.