[HPLC fingerprint of flavonoids and phenols of Dendrobium nobile].Zhong Yao Cai. 2009 Jun; 32(6):871-4.ZY
To establish HPLC fingerprint of flavonoids and phenols of Dendrobium nobile.
Phenomenex prodigy ODS(3) C18 column (250 mm x 4.6 mm, 5 microm) was used with a mixture of acetonitrile-0.1% acetic acid as the mobile phase in a gradient mode, the column temperature was 25 degrees C, the flow rate was 1.0 mL/min, and the detection wavelength was 254 nm.
The flavonoids and phenols of Dendrobium nobile were well separated, and 10 fingerprint peaks in common were confirmed.
This method is simple, accurate with good reproducibility, and can be used specifically for the quality control of Dendrobium nobile.