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[Simultaneous determination of anthraquinones in Liuwei'anxiao capsules by HPLC].
Zhong Yao Cai. 2009 Jun; 32(6):975-7.ZY

Abstract

OBJECTIVE

To establish an RP-HPLC method for measuring the contents of aloe-emodin, rhein, emodin, chrysophanol and physion in Liuwei'anxiao Capsules (traditional Chinese medicine).

METHODS

The analysis was carried out with a Diamonsil-C18 column (200 mm x 4.6 mm, 5 microm) and a mobile phase of methanol-0.05% phosphoric acid solution with gradient elution. The flow rate was 1.0 mL/min and the column temperature was 35 degrees C. The detection wavelength was 254 nm.

RESULTS

The calibration curve was linear within the range of 1.622 - 16.22 microg/mL (r1 = 0.9998), 0.6960 - 6.960 microg/mL (r2 = 0.9998), 2.448 - 24.48 microg/mL (r3 = 0.9995), 2.752 - 27.52 microg/mL (r4 = 0.9997), 2.702 -27.02 microg/mL (r = 0.9997) for aloe-emodin, rhein, emodin, chrysophanol and physion, respectively.

CONCLUSION

This method is convenient, accurate and reproducible for the quality control of Liuwei'anxiao Capsules.

Authors+Show Affiliations

Jiang B
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. rabbitbo@sina.com
Gao X
No affiliation info available
Bi KS
No affiliation info available
Chen XH
No affiliation info available

MeSH

AnthraquinonesCapsulesChromatography, High Pressure LiquidDrug CombinationsDrugs, Chinese HerbalEmodinPlants, MedicinalQuality ControlReproducibility of Results

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19764339

Citation

Jiang, Bo, et al. "[Simultaneous Determination of Anthraquinones in Liuwei'anxiao Capsules By HPLC]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 32, no. 6, 2009, pp. 975-7.
Jiang B, Gao X, Bi KS, et al. [Simultaneous determination of anthraquinones in Liuwei'anxiao capsules by HPLC]. Zhong Yao Cai. 2009;32(6):975-7.
Jiang, B., Gao, X., Bi, K. S., & Chen, X. H. (2009). [Simultaneous determination of anthraquinones in Liuwei'anxiao capsules by HPLC]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 32(6), 975-7.
Jiang B, et al. [Simultaneous Determination of Anthraquinones in Liuwei'anxiao Capsules By HPLC]. Zhong Yao Cai. 2009;32(6):975-7. PubMed PMID: 19764339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Simultaneous determination of anthraquinones in Liuwei'anxiao capsules by HPLC]. AU - Jiang,Bo, AU - Gao,Xun, AU - Bi,Kai-Shun, AU - Chen,Xiao-Hui, PY - 2009/9/22/entrez PY - 2009/9/22/pubmed PY - 2009/12/30/medline SP - 975 EP - 7 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 32 IS - 6 N2 - OBJECTIVE: To establish an RP-HPLC method for measuring the contents of aloe-emodin, rhein, emodin, chrysophanol and physion in Liuwei'anxiao Capsules (traditional Chinese medicine). METHODS: The analysis was carried out with a Diamonsil-C18 column (200 mm x 4.6 mm, 5 microm) and a mobile phase of methanol-0.05% phosphoric acid solution with gradient elution. The flow rate was 1.0 mL/min and the column temperature was 35 degrees C. The detection wavelength was 254 nm. RESULTS: The calibration curve was linear within the range of 1.622 - 16.22 microg/mL (r1 = 0.9998), 0.6960 - 6.960 microg/mL (r2 = 0.9998), 2.448 - 24.48 microg/mL (r3 = 0.9995), 2.752 - 27.52 microg/mL (r4 = 0.9997), 2.702 -27.02 microg/mL (r = 0.9997) for aloe-emodin, rhein, emodin, chrysophanol and physion, respectively. CONCLUSION: This method is convenient, accurate and reproducible for the quality control of Liuwei'anxiao Capsules. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/19764339/[Simultaneous_determination_of_anthraquinones_in_Liuwei'anxiao_capsules_by_HPLC]_ L2 - https://antibodies.cancer.gov/detail/CPTC-RRM2-2 DB - PRIME DP - Unbound Medicine ER -