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Surface plasmon resonance imaging for affinity-based biosensors.
Biosens Bioelectron. 2010 Jan 15; 25(5):957-66.BB

Abstract

SPR imaging (SPRi) is at the forefront of optical label-free and real-time detection. It offers the possibility of monitoring hundreds of biological interactions simultaneously and from the binding profiles, allows the estimation of the kinetic parameters of the interactions between the immobilised probes and the ligands in solution. We review the current state of development of SPRi technology and its application including commercially available SPRi instruments. Attention is also given to surface chemistries for biochip functionalisation and suitable approaches to improve sensitivity.

Authors+Show Affiliations

Dipartimento di Chimica, Università degli Studi di Firenze-Polo Scientifico, Sesto F.no (FI), Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

19765967

Citation

Scarano, Simona, et al. "Surface Plasmon Resonance Imaging for Affinity-based Biosensors." Biosensors & Bioelectronics, vol. 25, no. 5, 2010, pp. 957-66.
Scarano S, Mascini M, Turner AP, et al. Surface plasmon resonance imaging for affinity-based biosensors. Biosens Bioelectron. 2010;25(5):957-66.
Scarano, S., Mascini, M., Turner, A. P., & Minunni, M. (2010). Surface plasmon resonance imaging for affinity-based biosensors. Biosensors & Bioelectronics, 25(5), 957-66. https://doi.org/10.1016/j.bios.2009.08.039
Scarano S, et al. Surface Plasmon Resonance Imaging for Affinity-based Biosensors. Biosens Bioelectron. 2010 Jan 15;25(5):957-66. PubMed PMID: 19765967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface plasmon resonance imaging for affinity-based biosensors. AU - Scarano,Simona, AU - Mascini,Marco, AU - Turner,Anthony P F, AU - Minunni,Maria, Y1 - 2009/09/02/ PY - 2009/06/11/received PY - 2009/07/15/revised PY - 2009/08/26/accepted PY - 2009/9/22/entrez PY - 2009/9/22/pubmed PY - 2010/4/24/medline SP - 957 EP - 66 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 25 IS - 5 N2 - SPR imaging (SPRi) is at the forefront of optical label-free and real-time detection. It offers the possibility of monitoring hundreds of biological interactions simultaneously and from the binding profiles, allows the estimation of the kinetic parameters of the interactions between the immobilised probes and the ligands in solution. We review the current state of development of SPRi technology and its application including commercially available SPRi instruments. Attention is also given to surface chemistries for biochip functionalisation and suitable approaches to improve sensitivity. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/19765967/Surface_plasmon_resonance_imaging_for_affinity_based_biosensors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(09)00463-1 DB - PRIME DP - Unbound Medicine ER -