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Electrochemical behaviour of dimethyl-2-oxoglutarate on glassy carbon electrode.
Bioelectrochemistry. 2010 Feb; 77(2):145-50.B

Abstract

The electrochemical behaviour of dimethyl-2-oxoglutarate (MOG), a key intermediate in the Krebs cycle and an important nitrogen transporter in the metabolic pathways in biological processes, was investigated by cyclic voltammetry, square wave voltammetry and differential pulse voltammetry using a glassy carbon electrode. The reduction of MOG is an irreversible diffusion-controlled process that occurs in a cascade mechanism. For electrolytes with pH <3.0 and pH >7.0 one peak occurred and for 3.0<pH<8.0 two peaks corresponding to consecutive charge transfer reactions were observed. The effects of scan rate, concentration and pH of the electrolyte solution were monitored, and both peaks were found to shift cathodically with the increase in pH. DPV measurements allowed the determination of the number of electrons and protons i.e., one electron and one proton, involved in the reduction mechanism of MOG. Based upon the results obtained a reduction mechanism was proposed and the observed waves were attributed to the hydroxylation of the keto group of MOG to form dimethyl-2-hydroxyglutarate. Furthermore, two methodologies for the electroanalytical determination of MOG were also compared.

Authors+Show Affiliations

Departamento de Química, Universidade de Coimbra, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19766063

Citation

Shah, Afzal, et al. "Electrochemical Behaviour of Dimethyl-2-oxoglutarate On Glassy Carbon Electrode." Bioelectrochemistry (Amsterdam, Netherlands), vol. 77, no. 2, 2010, pp. 145-50.
Shah A, Diculescu VC, Qureshi R, et al. Electrochemical behaviour of dimethyl-2-oxoglutarate on glassy carbon electrode. Bioelectrochemistry. 2010;77(2):145-50.
Shah, A., Diculescu, V. C., Qureshi, R., & Oliveira-Brett, A. M. (2010). Electrochemical behaviour of dimethyl-2-oxoglutarate on glassy carbon electrode. Bioelectrochemistry (Amsterdam, Netherlands), 77(2), 145-50. https://doi.org/10.1016/j.bioelechem.2009.07.012
Shah A, et al. Electrochemical Behaviour of Dimethyl-2-oxoglutarate On Glassy Carbon Electrode. Bioelectrochemistry. 2010;77(2):145-50. PubMed PMID: 19766063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical behaviour of dimethyl-2-oxoglutarate on glassy carbon electrode. AU - Shah,Afzal, AU - Diculescu,Victor C, AU - Qureshi,Rumana, AU - Oliveira-Brett,Ana Maria, Y1 - 2009/08/12/ PY - 2009/06/16/received PY - 2009/07/25/revised PY - 2009/07/31/accepted PY - 2009/9/22/entrez PY - 2009/9/22/pubmed PY - 2010/7/14/medline SP - 145 EP - 50 JF - Bioelectrochemistry (Amsterdam, Netherlands) JO - Bioelectrochemistry VL - 77 IS - 2 N2 - The electrochemical behaviour of dimethyl-2-oxoglutarate (MOG), a key intermediate in the Krebs cycle and an important nitrogen transporter in the metabolic pathways in biological processes, was investigated by cyclic voltammetry, square wave voltammetry and differential pulse voltammetry using a glassy carbon electrode. The reduction of MOG is an irreversible diffusion-controlled process that occurs in a cascade mechanism. For electrolytes with pH <3.0 and pH >7.0 one peak occurred and for 3.0<pH<8.0 two peaks corresponding to consecutive charge transfer reactions were observed. The effects of scan rate, concentration and pH of the electrolyte solution were monitored, and both peaks were found to shift cathodically with the increase in pH. DPV measurements allowed the determination of the number of electrons and protons i.e., one electron and one proton, involved in the reduction mechanism of MOG. Based upon the results obtained a reduction mechanism was proposed and the observed waves were attributed to the hydroxylation of the keto group of MOG to form dimethyl-2-hydroxyglutarate. Furthermore, two methodologies for the electroanalytical determination of MOG were also compared. SN - 1878-562X UR - https://www.unboundmedicine.com/medline/citation/19766063/Electrochemical_behaviour_of_dimethyl_2_oxoglutarate_on_glassy_carbon_electrode_ DB - PRIME DP - Unbound Medicine ER -