Tags

Type your tag names separated by a space and hit enter

Molecular hydrogen suppresses FcepsilonRI-mediated signal transduction and prevents degranulation of mast cells.
Biochem Biophys Res Commun 2009; 389(4):651-6BB

Abstract

Molecular hydrogen ameliorates oxidative stress-associated diseases in animal models. We found that oral intake of hydrogen-rich water abolishes an immediate-type allergic reaction in mice. Using rat RBL-2H3 mast cells, we demonstrated that hydrogen attenuates phosphorylation of the FcepsilonRI-associated Lyn and its downstream signal transduction, which subsequently inhibits the NADPH oxidase activity and reduces the generation of hydrogen peroxide. We also found that inhibition of NADPH oxidase attenuates phosphorylation of Lyn in mast cells, indicating the presence of a feed-forward loop that potentiates the allergic responses. Hydrogen accordingly inhibits all tested signaling molecule(s) in the loop. Hydrogen effects have been solely ascribed to exclusive removal of hydroxyl radical. In the immediate-type allergic reaction, hydrogen exerts its beneficial effect not by its radical scavenging activity but by modulating a specific signaling pathway. Effects of hydrogen in other diseases are possibly mediated by modulation of yet unidentified signaling pathways. Our studies also suggest that hydrogen is a gaseous signaling molecule like nitric oxide.

Authors+Show Affiliations

Department of Longevity and Aging Research, Gifu International Institute of Biotechnology, 1-1 Naka-fudogaoka, Kakamigahara, Gifu, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19766097

Citation

Itoh, Tomohiro, et al. "Molecular Hydrogen Suppresses FcepsilonRI-mediated Signal Transduction and Prevents Degranulation of Mast Cells." Biochemical and Biophysical Research Communications, vol. 389, no. 4, 2009, pp. 651-6.
Itoh T, Fujita Y, Ito M, et al. Molecular hydrogen suppresses FcepsilonRI-mediated signal transduction and prevents degranulation of mast cells. Biochem Biophys Res Commun. 2009;389(4):651-6.
Itoh, T., Fujita, Y., Ito, M., Masuda, A., Ohno, K., Ichihara, M., ... Ito, M. (2009). Molecular hydrogen suppresses FcepsilonRI-mediated signal transduction and prevents degranulation of mast cells. Biochemical and Biophysical Research Communications, 389(4), pp. 651-6. doi:10.1016/j.bbrc.2009.09.047.
Itoh T, et al. Molecular Hydrogen Suppresses FcepsilonRI-mediated Signal Transduction and Prevents Degranulation of Mast Cells. Biochem Biophys Res Commun. 2009 Nov 27;389(4):651-6. PubMed PMID: 19766097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular hydrogen suppresses FcepsilonRI-mediated signal transduction and prevents degranulation of mast cells. AU - Itoh,Tomohiro, AU - Fujita,Yasunori, AU - Ito,Mikako, AU - Masuda,Akio, AU - Ohno,Kinji, AU - Ichihara,Masatoshi, AU - Kojima,Toshio, AU - Nozawa,Yoshinori, AU - Ito,Masafumi, Y1 - 2009/09/17/ PY - 2009/08/26/received PY - 2009/09/14/accepted PY - 2009/9/22/entrez PY - 2009/9/22/pubmed PY - 2009/11/3/medline SP - 651 EP - 6 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 389 IS - 4 N2 - Molecular hydrogen ameliorates oxidative stress-associated diseases in animal models. We found that oral intake of hydrogen-rich water abolishes an immediate-type allergic reaction in mice. Using rat RBL-2H3 mast cells, we demonstrated that hydrogen attenuates phosphorylation of the FcepsilonRI-associated Lyn and its downstream signal transduction, which subsequently inhibits the NADPH oxidase activity and reduces the generation of hydrogen peroxide. We also found that inhibition of NADPH oxidase attenuates phosphorylation of Lyn in mast cells, indicating the presence of a feed-forward loop that potentiates the allergic responses. Hydrogen accordingly inhibits all tested signaling molecule(s) in the loop. Hydrogen effects have been solely ascribed to exclusive removal of hydroxyl radical. In the immediate-type allergic reaction, hydrogen exerts its beneficial effect not by its radical scavenging activity but by modulating a specific signaling pathway. Effects of hydrogen in other diseases are possibly mediated by modulation of yet unidentified signaling pathways. Our studies also suggest that hydrogen is a gaseous signaling molecule like nitric oxide. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/19766097/abstract/Molecular_hydrogen_suppresses_FcepsilonRI_mediated_signal_transduction_and_prevents_degranulation_of_mast_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(09)01849-X DB - PRIME DP - Unbound Medicine ER -