Prime

Type your tag names separated by a space and hit enter

Molecular hydrogen suppresses FcepsilonRI-mediated signal transduction and prevents degranulation of mast cells.

Abstract

Molecular hydrogen ameliorates oxidative stress-associated diseases in animal models. We found that oral intake of hydrogen-rich water abolishes an immediate-type allergic reaction in mice. Using rat RBL-2H3 mast cells, we demonstrated that hydrogen attenuates phosphorylation of the FcepsilonRI-associated Lyn and its downstream signal transduction, which subsequently inhibits the NADPH oxidase activity and reduces the generation of hydrogen peroxide. We also found that inhibition of NADPH oxidase attenuates phosphorylation of Lyn in mast cells, indicating the presence of a feed-forward loop that potentiates the allergic responses. Hydrogen accordingly inhibits all tested signaling molecule(s) in the loop. Hydrogen effects have been solely ascribed to exclusive removal of hydroxyl radical. In the immediate-type allergic reaction, hydrogen exerts its beneficial effect not by its radical scavenging activity but by modulating a specific signaling pathway. Effects of hydrogen in other diseases are possibly mediated by modulation of yet unidentified signaling pathways. Our studies also suggest that hydrogen is a gaseous signaling molecule like nitric oxide.

Links

  • Publisher Full Text
  • Authors

    , , , , , , , ,

    Source

    MeSH

    Animals
    Cell Degranulation
    Cell Line, Tumor
    Hydrogen
    Mast Cells
    Mice
    Mice, Inbred ICR
    NADPH Oxidase
    Phosphorylation
    Rats
    Receptors, IgE
    Signal Transduction
    src-Family Kinases

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19766097

    Citation

    TY - JOUR T1 - Molecular hydrogen suppresses FcepsilonRI-mediated signal transduction and prevents degranulation of mast cells. AU - Itoh,Tomohiro, AU - Fujita,Yasunori, AU - Ito,Mikako, AU - Masuda,Akio, AU - Ohno,Kinji, AU - Ichihara,Masatoshi, AU - Kojima,Toshio, AU - Nozawa,Yoshinori, AU - Ito,Masafumi, Y1 - 2009/09/17/ PY - 2009/8/26/received PY - 2009/9/14/accepted PY - 2009/9/17/aheadofprint PY - 2009/9/22/entrez PY - 2009/9/22/pubmed PY - 2009/11/3/medline SP - 651 EP - 6 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 389 IS - 4 N2 - Molecular hydrogen ameliorates oxidative stress-associated diseases in animal models. We found that oral intake of hydrogen-rich water abolishes an immediate-type allergic reaction in mice. Using rat RBL-2H3 mast cells, we demonstrated that hydrogen attenuates phosphorylation of the FcepsilonRI-associated Lyn and its downstream signal transduction, which subsequently inhibits the NADPH oxidase activity and reduces the generation of hydrogen peroxide. We also found that inhibition of NADPH oxidase attenuates phosphorylation of Lyn in mast cells, indicating the presence of a feed-forward loop that potentiates the allergic responses. Hydrogen accordingly inhibits all tested signaling molecule(s) in the loop. Hydrogen effects have been solely ascribed to exclusive removal of hydroxyl radical. In the immediate-type allergic reaction, hydrogen exerts its beneficial effect not by its radical scavenging activity but by modulating a specific signaling pathway. Effects of hydrogen in other diseases are possibly mediated by modulation of yet unidentified signaling pathways. Our studies also suggest that hydrogen is a gaseous signaling molecule like nitric oxide. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/19766097/abstract/Molecular_hydrogen_suppresses_FcepsilonRI_mediated_signal_transduction_and_prevents_degranulation_of_mast_cells_ L2 - http://linkinghub.elsevier.com/retrieve/pii/S0006-291X(09)01849-X ER -