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Qualitative and quantitative analysis of steroidal saponins in crude extracts from Paris polyphylla var. yunnanensis and P. polyphylla var. chinensis by high performance liquid chromatography coupled with mass spectrometry.
J Pharm Biomed Anal. 2010 Jan 05; 51(1):114-24.JP

Abstract

High performance liquid chromatography coupled with electrospray ionization multi-stage tandem mass spectrometry (HPLC-ESI-MS(n)) and triple quadrupole mass spectrometric detection (HPLC-ESI-MS/MS), respectively, had been employed for the simultaneous identification and quantification of steroidal saponins in the rhizomes of Parispolyphylla var. yunnanensis and P. polyphylla var. chinensis, which are the qualified plants of "Chonglou" in Chinese. The HPLC experiments were performed by means of a reversed-phase C-18 column and a binary mobile phase system consisting of 0.1% aqueous formic acid and acetonitrile under gradient elution conditions. The characteristic fragmentation patterns of diosgenin- and pennogenin-type steroidal saponins were investigated using ESI-MS(n) in negative ion mode. The MS(n) data of the [M-H](-) ions provided structural information on the sugar sequence of the oligosaccharide chains and the aglycones of steroidal saponins. As a result, ten and seven saponins were determined in P. polyphylla var. yunnanensis and P. polyphylla var. chinensis, respectively, including four unknown compounds. One unknown compound was tentatively identified as diosgenin-3-O-alpha-L-rhamnopyranosyl(1-->4) [alpha-L-rhamnopyranosyl(1-->2)]-beta-D-glucopyranoside and the aglycones of the other three new compounds were reported from Chonglou for the first time. The developed HPLC-ESI-MS/MS method was validated and found to be satisfactorily linear, selective and robust. The limits of detection (LODs) and quantitation (LOQs) ranged, respectively, from 0.5 to 10 ng/mL and 2 to 34 ng/mL depending on six various compounds. The intra- and inter-day precisions of the method were evaluated and were less than 5.0%. Recoveries ranged from 92% to 104% for all compounds. The established quality evaluation method was successfully used for simultaneous quantification of six predominant steroidal saponins in the rhizomes of these two Paris species.

Authors+Show Affiliations

Murad Research Center for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

19767166

Citation

Zhang, Ting, et al. "Qualitative and Quantitative Analysis of Steroidal Saponins in Crude Extracts From Paris Polyphylla Var. Yunnanensis and P. Polyphylla Var. Chinensis By High Performance Liquid Chromatography Coupled With Mass Spectrometry." Journal of Pharmaceutical and Biomedical Analysis, vol. 51, no. 1, 2010, pp. 114-24.
Zhang T, Liu H, Liu XT, et al. Qualitative and quantitative analysis of steroidal saponins in crude extracts from Paris polyphylla var. yunnanensis and P. polyphylla var. chinensis by high performance liquid chromatography coupled with mass spectrometry. J Pharm Biomed Anal. 2010;51(1):114-24.
Zhang, T., Liu, H., Liu, X. T., Xu, D. R., Chen, X. Q., & Wang, Q. (2010). Qualitative and quantitative analysis of steroidal saponins in crude extracts from Paris polyphylla var. yunnanensis and P. polyphylla var. chinensis by high performance liquid chromatography coupled with mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 51(1), 114-24. https://doi.org/10.1016/j.jpba.2009.08.020
Zhang T, et al. Qualitative and Quantitative Analysis of Steroidal Saponins in Crude Extracts From Paris Polyphylla Var. Yunnanensis and P. Polyphylla Var. Chinensis By High Performance Liquid Chromatography Coupled With Mass Spectrometry. J Pharm Biomed Anal. 2010 Jan 5;51(1):114-24. PubMed PMID: 19767166.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Qualitative and quantitative analysis of steroidal saponins in crude extracts from Paris polyphylla var. yunnanensis and P. polyphylla var. chinensis by high performance liquid chromatography coupled with mass spectrometry. AU - Zhang,Ting, AU - Liu,Hai, AU - Liu,Xue-Ting, AU - Xu,De-ran, AU - Chen,Xiao-qing, AU - Wang,Qiang, Y1 - 2009/08/25/ PY - 2009/02/28/received PY - 2009/08/16/revised PY - 2009/08/18/accepted PY - 2009/9/22/entrez PY - 2009/9/22/pubmed PY - 2010/1/29/medline SP - 114 EP - 24 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 51 IS - 1 N2 - High performance liquid chromatography coupled with electrospray ionization multi-stage tandem mass spectrometry (HPLC-ESI-MS(n)) and triple quadrupole mass spectrometric detection (HPLC-ESI-MS/MS), respectively, had been employed for the simultaneous identification and quantification of steroidal saponins in the rhizomes of Parispolyphylla var. yunnanensis and P. polyphylla var. chinensis, which are the qualified plants of "Chonglou" in Chinese. The HPLC experiments were performed by means of a reversed-phase C-18 column and a binary mobile phase system consisting of 0.1% aqueous formic acid and acetonitrile under gradient elution conditions. The characteristic fragmentation patterns of diosgenin- and pennogenin-type steroidal saponins were investigated using ESI-MS(n) in negative ion mode. The MS(n) data of the [M-H](-) ions provided structural information on the sugar sequence of the oligosaccharide chains and the aglycones of steroidal saponins. As a result, ten and seven saponins were determined in P. polyphylla var. yunnanensis and P. polyphylla var. chinensis, respectively, including four unknown compounds. One unknown compound was tentatively identified as diosgenin-3-O-alpha-L-rhamnopyranosyl(1-->4) [alpha-L-rhamnopyranosyl(1-->2)]-beta-D-glucopyranoside and the aglycones of the other three new compounds were reported from Chonglou for the first time. The developed HPLC-ESI-MS/MS method was validated and found to be satisfactorily linear, selective and robust. The limits of detection (LODs) and quantitation (LOQs) ranged, respectively, from 0.5 to 10 ng/mL and 2 to 34 ng/mL depending on six various compounds. The intra- and inter-day precisions of the method were evaluated and were less than 5.0%. Recoveries ranged from 92% to 104% for all compounds. The established quality evaluation method was successfully used for simultaneous quantification of six predominant steroidal saponins in the rhizomes of these two Paris species. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/19767166/Qualitative_and_quantitative_analysis_of_steroidal_saponins_in_crude_extracts_from_Paris_polyphylla_var__yunnanensis_and_P__polyphylla_var__chinensis_by_high_performance_liquid_chromatography_coupled_with_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(09)00524-X DB - PRIME DP - Unbound Medicine ER -