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Modulatory action of potassium channel openers on field potential and histamine release from rat peritoneal mast cells.
Can J Physiol Pharmacol. 2009 Aug; 87(8):624-32.CJ

Abstract

To determine whether changes in membrane potential affect the extent of mast cell degranulation, compound 48/80 was added to rat peritoneal mast cell suspensions in the absence or presence of potassium channel openers (KCOs). Changes were compared between the field potential (FP) and the amount of histamine released. The results demonstrated that (i) the onset and duration of FP, which reflects the hyperpolarizing nature of the response, increased as the concentration of compound 48/80 increased; (ii) both FP and the amount of histamine released increased as the concentration of compound 48/80 increased; (iii) although both KCOs (SDZ PCO400, a benzopyran derivative, and P1060, a cyanoguanidine derivative) potentiated compound 48/80-induced increases in FP and histamine release, without compound 48/80, they had no effect on either parameter; (iv) both glibenclamide and charybdotoxin significantly attenuated the compound 48/80-induced increase in FP; and (v) glibenclamide was able to attenuate the KCO-induced potentiation of FP. The results show that drugs presumably causing hyperpolarization can affect histamine release from rat peritoneal mast cells. The effect of KCOs on compound 48/80-induced response appears to be potentiation in nature rather than synergism. It is possible that KCO hyperpolarizes the cell membrane, enhances Ca2+ influx, and thus increases histamine release. As such, selective blockers of K+ channels may be useful for the treatment of immunological disorders.

Authors+Show Affiliations

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong. yeungck@rocketmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19767887

Citation

Yeung, Chi-Kong, et al. "Modulatory Action of Potassium Channel Openers On Field Potential and Histamine Release From Rat Peritoneal Mast Cells." Canadian Journal of Physiology and Pharmacology, vol. 87, no. 8, 2009, pp. 624-32.
Yeung CK, Law JK, Sam SW, et al. Modulatory action of potassium channel openers on field potential and histamine release from rat peritoneal mast cells. Can J Physiol Pharmacol. 2009;87(8):624-32.
Yeung, C. K., Law, J. K., Sam, S. W., Ingebrandt, S., Lau, H. Y., Rudd, J. A., & Chan, M. (2009). Modulatory action of potassium channel openers on field potential and histamine release from rat peritoneal mast cells. Canadian Journal of Physiology and Pharmacology, 87(8), 624-32. https://doi.org/10.1139/y09-047
Yeung CK, et al. Modulatory Action of Potassium Channel Openers On Field Potential and Histamine Release From Rat Peritoneal Mast Cells. Can J Physiol Pharmacol. 2009;87(8):624-32. PubMed PMID: 19767887.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulatory action of potassium channel openers on field potential and histamine release from rat peritoneal mast cells. AU - Yeung,Chi-Kong, AU - Law,Jessica Ka-Yan, AU - Sam,Sze-Wing, AU - Ingebrandt,Sven, AU - Lau,Hang-Yung Alaster, AU - Rudd,John Anthony, AU - Chan,Mansun, PY - 2009/9/22/entrez PY - 2009/9/22/pubmed PY - 2010/5/7/medline SP - 624 EP - 32 JF - Canadian journal of physiology and pharmacology JO - Can J Physiol Pharmacol VL - 87 IS - 8 N2 - To determine whether changes in membrane potential affect the extent of mast cell degranulation, compound 48/80 was added to rat peritoneal mast cell suspensions in the absence or presence of potassium channel openers (KCOs). Changes were compared between the field potential (FP) and the amount of histamine released. The results demonstrated that (i) the onset and duration of FP, which reflects the hyperpolarizing nature of the response, increased as the concentration of compound 48/80 increased; (ii) both FP and the amount of histamine released increased as the concentration of compound 48/80 increased; (iii) although both KCOs (SDZ PCO400, a benzopyran derivative, and P1060, a cyanoguanidine derivative) potentiated compound 48/80-induced increases in FP and histamine release, without compound 48/80, they had no effect on either parameter; (iv) both glibenclamide and charybdotoxin significantly attenuated the compound 48/80-induced increase in FP; and (v) glibenclamide was able to attenuate the KCO-induced potentiation of FP. The results show that drugs presumably causing hyperpolarization can affect histamine release from rat peritoneal mast cells. The effect of KCOs on compound 48/80-induced response appears to be potentiation in nature rather than synergism. It is possible that KCO hyperpolarizes the cell membrane, enhances Ca2+ influx, and thus increases histamine release. As such, selective blockers of K+ channels may be useful for the treatment of immunological disorders. SN - 1205-7541 UR - https://www.unboundmedicine.com/medline/citation/19767887/Modulatory_action_of_potassium_channel_openers_on_field_potential_and_histamine_release_from_rat_peritoneal_mast_cells_ L2 - https://cdnsciencepub.com/doi/10.1139/y09-047?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -