Tags

Type your tag names separated by a space and hit enter

Surface plasmon resonance biosensor for direct detection of antibodies against human growth hormone.
Analyst. 2009 Oct; 134(10):2051-7.A

Abstract

A direct, label-free detection method of antibodies against the human growth hormone (anti-HGH) using a surface plasmon resonance (SPR) biosensor is reported. The sensing surface of the surface plasmon resonance biosensor chip (SPR-chip) was modified by covalent coupling of the human growth hormone (HGH) to the self-assembled monolayer of 11-mercaptoundecanoic acid (MUA). HGH was immobilized via primary amine groups after activation of the MUA carboxyl groups with a mixture of N-hydroxysuccinimide (NHS), and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC). The specific binding of monoclonal anti-HGH antibody on the HGH-modified surface was examined in the concentration range from 0.25 nM to 10 microM. The experimentally observed detection minimum for anti-HGH was 2.47 nM. A single immunoassay cycle could be done within 30 min including the HGH and anti-HGH association, HGH/anti-HGH complex dissociation and surface regeneration steps. The SPR biosensor response for repeatable detections of anti-HGH was highly reproducible and very stable. On the SPR-chip the formed HGH and anti-HGH complex (HGH/anti-HGH) could be gently dissociated and the sensing surface might be regenerated by 50 mM NaOH and 0.5% sodium dodecylsulfate (SDS) solution. Any changes in the original baseline level were detected during the 40 detection-regeneration cycles. This means that damage of the immobilized HGH-based sensitive layer during regeneration was minimal. It was demonstrated that the developed SPR-chip could be stored for at least 21 days before use without considerable loss of sensitivity towards anti-HGH.

Authors+Show Affiliations

Centre of Nanotechnology and Material science-Nanotechnas, Faculty of Chemistry, Vilnius University, Naugarduko 24, LT-03225, Vilnius 6, Lithuania. astakausaite@imi.ltNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19768212

Citation

Kausaite-Minkstimiene, Asta, et al. "Surface Plasmon Resonance Biosensor for Direct Detection of Antibodies Against Human Growth Hormone." The Analyst, vol. 134, no. 10, 2009, pp. 2051-7.
Kausaite-Minkstimiene A, Ramanaviciene A, Ramanavicius A. Surface plasmon resonance biosensor for direct detection of antibodies against human growth hormone. Analyst. 2009;134(10):2051-7.
Kausaite-Minkstimiene, A., Ramanaviciene, A., & Ramanavicius, A. (2009). Surface plasmon resonance biosensor for direct detection of antibodies against human growth hormone. The Analyst, 134(10), 2051-7. https://doi.org/10.1039/b907315a
Kausaite-Minkstimiene A, Ramanaviciene A, Ramanavicius A. Surface Plasmon Resonance Biosensor for Direct Detection of Antibodies Against Human Growth Hormone. Analyst. 2009;134(10):2051-7. PubMed PMID: 19768212.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface plasmon resonance biosensor for direct detection of antibodies against human growth hormone. AU - Kausaite-Minkstimiene,Asta, AU - Ramanaviciene,Almira, AU - Ramanavicius,Arunas, Y1 - 2009/07/31/ PY - 2009/9/22/entrez PY - 2009/9/22/pubmed PY - 2010/3/27/medline SP - 2051 EP - 7 JF - The Analyst JO - Analyst VL - 134 IS - 10 N2 - A direct, label-free detection method of antibodies against the human growth hormone (anti-HGH) using a surface plasmon resonance (SPR) biosensor is reported. The sensing surface of the surface plasmon resonance biosensor chip (SPR-chip) was modified by covalent coupling of the human growth hormone (HGH) to the self-assembled monolayer of 11-mercaptoundecanoic acid (MUA). HGH was immobilized via primary amine groups after activation of the MUA carboxyl groups with a mixture of N-hydroxysuccinimide (NHS), and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC). The specific binding of monoclonal anti-HGH antibody on the HGH-modified surface was examined in the concentration range from 0.25 nM to 10 microM. The experimentally observed detection minimum for anti-HGH was 2.47 nM. A single immunoassay cycle could be done within 30 min including the HGH and anti-HGH association, HGH/anti-HGH complex dissociation and surface regeneration steps. The SPR biosensor response for repeatable detections of anti-HGH was highly reproducible and very stable. On the SPR-chip the formed HGH and anti-HGH complex (HGH/anti-HGH) could be gently dissociated and the sensing surface might be regenerated by 50 mM NaOH and 0.5% sodium dodecylsulfate (SDS) solution. Any changes in the original baseline level were detected during the 40 detection-regeneration cycles. This means that damage of the immobilized HGH-based sensitive layer during regeneration was minimal. It was demonstrated that the developed SPR-chip could be stored for at least 21 days before use without considerable loss of sensitivity towards anti-HGH. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/19768212/Surface_plasmon_resonance_biosensor_for_direct_detection_of_antibodies_against_human_growth_hormone_ DB - PRIME DP - Unbound Medicine ER -