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Graphite nanosheet-based composites for mediator-free H2O2 biosensor.
Analyst. 2009 Oct; 134(10):2135-40.A

Abstract

Graphite nanosheet (GNS)-Nafion composites were prepared for the immobilization of hemoglobin (Hb) to construct a mediator-free H(2)O(2) biosensor. Scanning electron microscopy (SEM), UV-vis spectroscopy and Fourier transform infrared spectroscopy were used to characterize the Hb-GNS-Nafion composite film. Due to good biocompatibility of the composite film, immobilized Hb retained its native structure. The Hb-GNS-Nafion composite film-modified electrodes showed direct electrochemistry with a fast electron-transfer rate (6.63 s(-1)) and high electrocatalytic activity to the reduction of hydrogen peroxide (H(2)O(2)). The resulting biosensor exhibited a high sensitivity of 412.7 mA M(-1) cm(-2), a low detection limit of 2.0 x 10(-7) M, and a small apparent Michaelis-Menten constant of 37 microM. These results were comparable or superior to the biosensors based on the carbon nanofiber (CNF)- and carbon nanotube (CNT)-based biosensors. Due to the lower cost of GNSs, the GNS-based composite can combine with other redox proteins and widely apply in mediator-free biosensors, bioelectronics and biofuel cells.

Authors+Show Affiliations

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19768226

Citation

Chen, Xu, et al. "Graphite Nanosheet-based Composites for Mediator-free H2O2 Biosensor." The Analyst, vol. 134, no. 10, 2009, pp. 2135-40.
Chen X, Fu C, Yang W. Graphite nanosheet-based composites for mediator-free H2O2 biosensor. Analyst. 2009;134(10):2135-40.
Chen, X., Fu, C., & Yang, W. (2009). Graphite nanosheet-based composites for mediator-free H2O2 biosensor. The Analyst, 134(10), 2135-40. https://doi.org/10.1039/b910754a
Chen X, Fu C, Yang W. Graphite Nanosheet-based Composites for Mediator-free H2O2 Biosensor. Analyst. 2009;134(10):2135-40. PubMed PMID: 19768226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Graphite nanosheet-based composites for mediator-free H2O2 biosensor. AU - Chen,Xu, AU - Fu,Chenglin, AU - Yang,Wensheng, Y1 - 2009/08/19/ PY - 2009/9/22/entrez PY - 2009/9/22/pubmed PY - 2010/3/27/medline SP - 2135 EP - 40 JF - The Analyst JO - Analyst VL - 134 IS - 10 N2 - Graphite nanosheet (GNS)-Nafion composites were prepared for the immobilization of hemoglobin (Hb) to construct a mediator-free H(2)O(2) biosensor. Scanning electron microscopy (SEM), UV-vis spectroscopy and Fourier transform infrared spectroscopy were used to characterize the Hb-GNS-Nafion composite film. Due to good biocompatibility of the composite film, immobilized Hb retained its native structure. The Hb-GNS-Nafion composite film-modified electrodes showed direct electrochemistry with a fast electron-transfer rate (6.63 s(-1)) and high electrocatalytic activity to the reduction of hydrogen peroxide (H(2)O(2)). The resulting biosensor exhibited a high sensitivity of 412.7 mA M(-1) cm(-2), a low detection limit of 2.0 x 10(-7) M, and a small apparent Michaelis-Menten constant of 37 microM. These results were comparable or superior to the biosensors based on the carbon nanofiber (CNF)- and carbon nanotube (CNT)-based biosensors. Due to the lower cost of GNSs, the GNS-based composite can combine with other redox proteins and widely apply in mediator-free biosensors, bioelectronics and biofuel cells. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/19768226/Graphite_nanosheet_based_composites_for_mediator_free_H2O2_biosensor_ L2 - https://doi.org/10.1039/b910754a DB - PRIME DP - Unbound Medicine ER -