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The topographical effect of electrospun nanofibrous scaffolds on the in vivo and in vitro foreign body reaction.
J Biomed Mater Res A 2010; 93(3):1151-9JB

Abstract

Topographical cues play an important role in influencing cellular behavior and are considered as significant parameters to be controlled in tissue engineering applications. This work investigated the biocompatibility with regard to scaffold architecture and topographical effect of nanofibrous scaffolds on the in vivo and in vitro foreign body reaction. Random and aligned polycaprolactone (PCL) nanofibers were fabricated by electrospinning technique, with diameters of 313 +/- 5 nm and 506 +/- 24 nm, respectively. Primary monocytes isolated from five human donors were cultured on PCL nanofibers, PCL film, and RGD-coated glass in vitro and cell density and morphology was evaluated at time points of day 0 (2 h), day 3, day 7, and day 10. The in vivo study was carried out by implanting PCL nanofibers and film scaffolds subcutaneously in rats to test the biocompatibility and host response at time points of week 1, week 2, and week 4. The in vitro studies revealed that the initial monocyte adhesion on the aligned fiber scaffold was significantly less (p < 0.001) when compared to the random fiber scaffold. The in vivo study showed that the thicknesses of fibrous capsule on fibrous scaffolds were 7.55 +/- 0.54 microm for aligned fibers and 4.13 +/- 0.31 microm for random fibers, which were significantly thinner than that of film implants 37.7 +/- 0.25 microm (p < 0.001). Additionally, cell infiltration was observed in aligned fibrous scaffolds both in vitro and in vivo, while on random fibers and films, distinct fibrous capsule boundaries were found on the surfaces. These results indicate that aligned electrospun nanofibers may serve as a promising scaffold for tissue engineering by minimizing host response, enhancing tissue-scaffold integration, and eliciting a thinner fibrous capsule.

Authors+Show Affiliations

School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19768795

Citation

Cao, Haoqing, et al. "The Topographical Effect of Electrospun Nanofibrous Scaffolds On the in Vivo and in Vitro Foreign Body Reaction." Journal of Biomedical Materials Research. Part A, vol. 93, no. 3, 2010, pp. 1151-9.
Cao H, McHugh K, Chew SY, et al. The topographical effect of electrospun nanofibrous scaffolds on the in vivo and in vitro foreign body reaction. J Biomed Mater Res A. 2010;93(3):1151-9.
Cao, H., McHugh, K., Chew, S. Y., & Anderson, J. M. (2010). The topographical effect of electrospun nanofibrous scaffolds on the in vivo and in vitro foreign body reaction. Journal of Biomedical Materials Research. Part A, 93(3), pp. 1151-9. doi:10.1002/jbm.a.32609.
Cao H, et al. The Topographical Effect of Electrospun Nanofibrous Scaffolds On the in Vivo and in Vitro Foreign Body Reaction. J Biomed Mater Res A. 2010 Jun 1;93(3):1151-9. PubMed PMID: 19768795.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The topographical effect of electrospun nanofibrous scaffolds on the in vivo and in vitro foreign body reaction. AU - Cao,Haoqing, AU - McHugh,Kevin, AU - Chew,Sing Yian, AU - Anderson,James M, PY - 2009/9/22/entrez PY - 2009/9/22/pubmed PY - 2010/7/9/medline SP - 1151 EP - 9 JF - Journal of biomedical materials research. Part A JO - J Biomed Mater Res A VL - 93 IS - 3 N2 - Topographical cues play an important role in influencing cellular behavior and are considered as significant parameters to be controlled in tissue engineering applications. This work investigated the biocompatibility with regard to scaffold architecture and topographical effect of nanofibrous scaffolds on the in vivo and in vitro foreign body reaction. Random and aligned polycaprolactone (PCL) nanofibers were fabricated by electrospinning technique, with diameters of 313 +/- 5 nm and 506 +/- 24 nm, respectively. Primary monocytes isolated from five human donors were cultured on PCL nanofibers, PCL film, and RGD-coated glass in vitro and cell density and morphology was evaluated at time points of day 0 (2 h), day 3, day 7, and day 10. The in vivo study was carried out by implanting PCL nanofibers and film scaffolds subcutaneously in rats to test the biocompatibility and host response at time points of week 1, week 2, and week 4. The in vitro studies revealed that the initial monocyte adhesion on the aligned fiber scaffold was significantly less (p < 0.001) when compared to the random fiber scaffold. The in vivo study showed that the thicknesses of fibrous capsule on fibrous scaffolds were 7.55 +/- 0.54 microm for aligned fibers and 4.13 +/- 0.31 microm for random fibers, which were significantly thinner than that of film implants 37.7 +/- 0.25 microm (p < 0.001). Additionally, cell infiltration was observed in aligned fibrous scaffolds both in vitro and in vivo, while on random fibers and films, distinct fibrous capsule boundaries were found on the surfaces. These results indicate that aligned electrospun nanofibers may serve as a promising scaffold for tissue engineering by minimizing host response, enhancing tissue-scaffold integration, and eliciting a thinner fibrous capsule. SN - 1552-4965 UR - https://www.unboundmedicine.com/medline/citation/19768795/The_topographical_effect_of_electrospun_nanofibrous_scaffolds_on_the_in_vivo_and_in_vitro_foreign_body_reaction_ L2 - https://doi.org/10.1002/jbm.a.32609 DB - PRIME DP - Unbound Medicine ER -