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Chrysanthemum morifolium Ramat (CM) extract protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity.
J Ethnopharmacol. 2009 Dec 10; 126(3):447-54.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Chrysanthemum morifolium Ramat (Asteraceae) has (CM) long been used in Korean and Chinese traditional herbal medicines with numerous therapeutic applications.

AIM OF THE STUDY

To evaluate the neuroprotective activities of Chrysanthemum morifolium (CM) extract against 1-methyl-4-phenylpridinium ions (MPP(+)), Parkinsonian toxin through oxidative stress and impaired energy metabolism, in human SH-SY5Y neuroblastoma cells and the underlying mechanisms.

MATERIALS AND METHODS

The effects of CM against MPP(+)-induced cytotoxicity and neuronal cell viability, oxidative damage, the expression of Bcl-2 and Bax, caspase-3 and poly(ADP-ribose) polymerase (PARP) proteolysis were evaluated by using SH-SY5Y neuroblastoma cells.

RESULTS

CM effectively inhibited the cytotoxicity and improved cell viability. CM also attenuated the elevation of reactive oxygen species (ROS) level, increase in Bax/Bcl-2 ratio, cleavage of caspase-3 and PARP proteolysis.

CONCLUSION

These results demonstrate that CM possesses potent neuroprotective activity and therefore, might be a potential candidate in neurodegenerative diseases such as Parkinson's disease.

Authors+Show Affiliations

Department of Biotechnology, Konkuk University, 322 Danwol-dong, Chungju 380-701, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19770030

Citation

Kim, In Su, et al. "Chrysanthemum Morifolium Ramat (CM) Extract Protects Human Neuroblastoma SH-SY5Y Cells Against MPP+-induced Cytotoxicity." Journal of Ethnopharmacology, vol. 126, no. 3, 2009, pp. 447-54.
Kim IS, Koppula S, Park PJ, et al. Chrysanthemum morifolium Ramat (CM) extract protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. J Ethnopharmacol. 2009;126(3):447-54.
Kim, I. S., Koppula, S., Park, P. J., Kim, E. H., Kim, C. G., Choi, W. S., Lee, K. H., & Choi, D. K. (2009). Chrysanthemum morifolium Ramat (CM) extract protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. Journal of Ethnopharmacology, 126(3), 447-54. https://doi.org/10.1016/j.jep.2009.09.017
Kim IS, et al. Chrysanthemum Morifolium Ramat (CM) Extract Protects Human Neuroblastoma SH-SY5Y Cells Against MPP+-induced Cytotoxicity. J Ethnopharmacol. 2009 Dec 10;126(3):447-54. PubMed PMID: 19770030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chrysanthemum morifolium Ramat (CM) extract protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. AU - Kim,In Su, AU - Koppula,Sushruta, AU - Park,Pyo-Jam, AU - Kim,Ee Hwa, AU - Kim,Chan Gil, AU - Choi,Wahn Soo, AU - Lee,Kwang Ho, AU - Choi,Dong-Kug, Y1 - 2009/09/19/ PY - 2009/04/09/received PY - 2009/08/18/revised PY - 2009/09/14/accepted PY - 2009/9/23/entrez PY - 2009/9/23/pubmed PY - 2010/2/19/medline SP - 447 EP - 54 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 126 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Chrysanthemum morifolium Ramat (Asteraceae) has (CM) long been used in Korean and Chinese traditional herbal medicines with numerous therapeutic applications. AIM OF THE STUDY: To evaluate the neuroprotective activities of Chrysanthemum morifolium (CM) extract against 1-methyl-4-phenylpridinium ions (MPP(+)), Parkinsonian toxin through oxidative stress and impaired energy metabolism, in human SH-SY5Y neuroblastoma cells and the underlying mechanisms. MATERIALS AND METHODS: The effects of CM against MPP(+)-induced cytotoxicity and neuronal cell viability, oxidative damage, the expression of Bcl-2 and Bax, caspase-3 and poly(ADP-ribose) polymerase (PARP) proteolysis were evaluated by using SH-SY5Y neuroblastoma cells. RESULTS: CM effectively inhibited the cytotoxicity and improved cell viability. CM also attenuated the elevation of reactive oxygen species (ROS) level, increase in Bax/Bcl-2 ratio, cleavage of caspase-3 and PARP proteolysis. CONCLUSION: These results demonstrate that CM possesses potent neuroprotective activity and therefore, might be a potential candidate in neurodegenerative diseases such as Parkinson's disease. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19770030/Chrysanthemum_morifolium_Ramat__CM__extract_protects_human_neuroblastoma_SH_SY5Y_cells_against_MPP+_induced_cytotoxicity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00577-7 DB - PRIME DP - Unbound Medicine ER -