Tags

Type your tag names separated by a space and hit enter

[Study on HPLC fingerprint of flavonoids from Houttuynia cordata by comparing with fingerprint reference].
Zhong Yao Cai. 2009 May; 32(5):687-90.ZY

Abstract

OBJECTIVE

To establish a stable and repeatable HPLC fingerprint standard and evaluate the flavonoids from Houttuynia cordata qualitatively and quantitatively.

METHODS

HPLC separation was performed on a C18 column with methanol-0.1% phosphoric acid mixed solution as mobile phase in gradient elution mode. The fingerprint reference was determined as one of the most typical chromatograms and used to be compared with other samples through Cosine and Relative Euclid Distance methods, thus the chromatographic fingerprints of flavonoids from Houttuynia cordata were evaluated by constitutes and contents, respectively.

RESULTS

Fourteen mutual peaks were fixed in the HPLC fingerprint of flavonoids from Houttaynia cordata. It showed good results in validation tests in which the quercitrin's peak was set as the reference peak to calculate relative retention time and area of other peaks in the chromatograms, and the RSD were less than 0.2% and 5.0%, respectively. The linear ranges for quercitrin was 1.07-83.4 microg/mL (r=0.9999) and the average recovery was 100.3%.

CONCLUSION

The method shows good repeatability, ruggedness and reliability. Comparing with the established reference fingerprint, the evaluation system including Cosine and Relative Euclid Distance methods lays dependable foundation for controlling the quality of Houttuynia cordata.

Authors+Show Affiliations

Zhang TT
Nanjing Municipal Second Hospital Affiliated to Medical College of Southeast University, Nanjing 210003, China. tingtingzh826@hotmail.com
Wu Y
No affiliation info available
Hang TJ
No affiliation info available

MeSH

Chromatography, High Pressure LiquidDrugs, Chinese HerbalFlavonoidsHouttuyniaPlants, MedicinalQuality ControlReproducibility of Results

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19771839

Citation

Zhang, Ting-Ting, et al. "[Study On HPLC Fingerprint of Flavonoids From Houttuynia Cordata By Comparing With Fingerprint Reference]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 32, no. 5, 2009, pp. 687-90.
Zhang TT, Wu Y, Hang TJ. [Study on HPLC fingerprint of flavonoids from Houttuynia cordata by comparing with fingerprint reference]. Zhong Yao Cai. 2009;32(5):687-90.
Zhang, T. T., Wu, Y., & Hang, T. J. (2009). [Study on HPLC fingerprint of flavonoids from Houttuynia cordata by comparing with fingerprint reference]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 32(5), 687-90.
Zhang TT, Wu Y, Hang TJ. [Study On HPLC Fingerprint of Flavonoids From Houttuynia Cordata By Comparing With Fingerprint Reference]. Zhong Yao Cai. 2009;32(5):687-90. PubMed PMID: 19771839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on HPLC fingerprint of flavonoids from Houttuynia cordata by comparing with fingerprint reference]. AU - Zhang,Ting-Ting, AU - Wu,Yi, AU - Hang,Tai-Jun, PY - 2009/9/24/entrez PY - 2009/9/24/pubmed PY - 2009/12/30/medline SP - 687 EP - 90 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 32 IS - 5 N2 - OBJECTIVE: To establish a stable and repeatable HPLC fingerprint standard and evaluate the flavonoids from Houttuynia cordata qualitatively and quantitatively. METHODS: HPLC separation was performed on a C18 column with methanol-0.1% phosphoric acid mixed solution as mobile phase in gradient elution mode. The fingerprint reference was determined as one of the most typical chromatograms and used to be compared with other samples through Cosine and Relative Euclid Distance methods, thus the chromatographic fingerprints of flavonoids from Houttuynia cordata were evaluated by constitutes and contents, respectively. RESULTS: Fourteen mutual peaks were fixed in the HPLC fingerprint of flavonoids from Houttaynia cordata. It showed good results in validation tests in which the quercitrin's peak was set as the reference peak to calculate relative retention time and area of other peaks in the chromatograms, and the RSD were less than 0.2% and 5.0%, respectively. The linear ranges for quercitrin was 1.07-83.4 microg/mL (r=0.9999) and the average recovery was 100.3%. CONCLUSION: The method shows good repeatability, ruggedness and reliability. Comparing with the established reference fingerprint, the evaluation system including Cosine and Relative Euclid Distance methods lays dependable foundation for controlling the quality of Houttuynia cordata. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/19771839/[Study_on_HPLC_fingerprint_of_flavonoids_from_Houttuynia_cordata_by_comparing_with_fingerprint_reference]_ DB - PRIME DP - Unbound Medicine ER -