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Role of nanofibrous poly(caprolactone) scaffolds in human mesenchymal stem cell attachment and spreading for in vitro bone tissue engineering--response to osteogenic regulators.
Tissue Eng Part A 2010; 16(2):393-404TE

Abstract

In this study, we evaluated the role of fiber size scale in the adhesion and spreading potential of human mesenchymal stem cells (hMSCs) on electrospun poly(caprolactone) (PCL) nanofibrous and microfibrous scaffolds. The effect of in vivo regulators in inducing osteogenic differentiation of hMSCs on PCL nanofibrous scaffolds was investigated using osteogenic differentiation marker gene expression and matrix mineralization. Here, we report for the first time the influence of in vivo regulators in an in vitro setting with hMSCs for bone tissue engineering on PCL nanofibrous matrices. Our results indicated that hMSCs attached and spread rapidly on nanofibrous scaffolds in comparison to microfibrous PCL. Further, hMSCs proliferated well on the nanofibrous scaffolds. The cells on the nanofibrous PCL were found to differentiate into the osteoblast lineage and subsequently mineralize upon addition of in vivo osteogenic regulators. The attachment and spreading of hMSCs were more effective on the nanofibers compared with the microfibers despite the lower protein surface coverage (total adsorbed protein per unit fiber surface area) on nanofibers.

Authors+Show Affiliations

Amrita Centre for Nanosciences, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19772455

Citation

Binulal, N S., et al. "Role of Nanofibrous Poly(caprolactone) Scaffolds in Human Mesenchymal Stem Cell Attachment and Spreading for in Vitro Bone Tissue Engineering--response to Osteogenic Regulators." Tissue Engineering. Part A, vol. 16, no. 2, 2010, pp. 393-404.
Binulal NS, Deepthy M, Selvamurugan N, et al. Role of nanofibrous poly(caprolactone) scaffolds in human mesenchymal stem cell attachment and spreading for in vitro bone tissue engineering--response to osteogenic regulators. Tissue Eng Part A. 2010;16(2):393-404.
Binulal, N. S., Deepthy, M., Selvamurugan, N., Shalumon, K. T., Suja, S., Mony, U., ... Nair, S. V. (2010). Role of nanofibrous poly(caprolactone) scaffolds in human mesenchymal stem cell attachment and spreading for in vitro bone tissue engineering--response to osteogenic regulators. Tissue Engineering. Part A, 16(2), pp. 393-404. doi:10.1089/ten.TEA.2009.0242.
Binulal NS, et al. Role of Nanofibrous Poly(caprolactone) Scaffolds in Human Mesenchymal Stem Cell Attachment and Spreading for in Vitro Bone Tissue Engineering--response to Osteogenic Regulators. Tissue Eng Part A. 2010;16(2):393-404. PubMed PMID: 19772455.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of nanofibrous poly(caprolactone) scaffolds in human mesenchymal stem cell attachment and spreading for in vitro bone tissue engineering--response to osteogenic regulators. AU - Binulal,N S, AU - Deepthy,M, AU - Selvamurugan,N, AU - Shalumon,K T, AU - Suja,S, AU - Mony,Ullas, AU - Jayakumar,R, AU - Nair,S V, PY - 2009/9/24/entrez PY - 2009/9/24/pubmed PY - 2010/9/29/medline SP - 393 EP - 404 JF - Tissue engineering. Part A JO - Tissue Eng Part A VL - 16 IS - 2 N2 - In this study, we evaluated the role of fiber size scale in the adhesion and spreading potential of human mesenchymal stem cells (hMSCs) on electrospun poly(caprolactone) (PCL) nanofibrous and microfibrous scaffolds. The effect of in vivo regulators in inducing osteogenic differentiation of hMSCs on PCL nanofibrous scaffolds was investigated using osteogenic differentiation marker gene expression and matrix mineralization. Here, we report for the first time the influence of in vivo regulators in an in vitro setting with hMSCs for bone tissue engineering on PCL nanofibrous matrices. Our results indicated that hMSCs attached and spread rapidly on nanofibrous scaffolds in comparison to microfibrous PCL. Further, hMSCs proliferated well on the nanofibrous scaffolds. The cells on the nanofibrous PCL were found to differentiate into the osteoblast lineage and subsequently mineralize upon addition of in vivo osteogenic regulators. The attachment and spreading of hMSCs were more effective on the nanofibers compared with the microfibers despite the lower protein surface coverage (total adsorbed protein per unit fiber surface area) on nanofibers. SN - 1937-335X UR - https://www.unboundmedicine.com/medline/citation/19772455/Role_of_nanofibrous_poly_caprolactone__scaffolds_in_human_mesenchymal_stem_cell_attachment_and_spreading_for_in_vitro_bone_tissue_engineering__response_to_osteogenic_regulators_ L2 - https://www.liebertpub.com/doi/full/10.1089/ten.TEA.2009.0242?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -