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Anti-inflammatory effects of madecassic acid via the suppression of NF-kappaB pathway in LPS-induced RAW 264.7 macrophage cells.
Planta Med. 2010 Feb; 76(3):251-7.PM

Abstract

We have investigated the anti-inflammatory effects of madecassic acid and madecassoside isolated from Centella asiatica (Umbelliferae) on lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cells. Both madecassic acid and madecassoside inhibited the production of nitric oxide (NO), prostaglandin E(2) (PGE(2)), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1beta), and IL-6. However, madecassic acid more potently suppressed these inflammatory mediators than did madecassoside. Consistent with these observations, madecassic acid inhibited the LPS-induced expression of iNOS and COX-2 at the protein level and of iNOS, COX-2, TNF-alpha, IL-1beta, and IL-6 at the mRNA level in RAW 264.7 macrophage cells, as determined by Western blotting and RT-PCR, respectively. Furthermore, madecassic acid suppressed the LPS-induced activation of nuclear factor-kappaB (NF-kappaB), and this was associated with the abrogation of inhibitory kappa B-alpha (IkappaB-alpha) degradation and with the subsequent blocking of p65 protein translocation to the nucleus. These results suggest that the anti-inflammatory properties of madecassic acid are caused by iNOS, COX-2, TNF-alpha, IL-1beta, and IL-6 inhibition via the downregulation of NF-kappaB activation in RAW 264.7 macrophage cells.

Authors+Show Affiliations

College of Pharmacy, Kyung-Hee University, Hoegi-Dong, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19774506

Citation

Won, Jong-Heon, et al. "Anti-inflammatory Effects of Madecassic Acid Via the Suppression of NF-kappaB Pathway in LPS-induced RAW 264.7 Macrophage Cells." Planta Medica, vol. 76, no. 3, 2010, pp. 251-7.
Won JH, Shin JS, Park HJ, et al. Anti-inflammatory effects of madecassic acid via the suppression of NF-kappaB pathway in LPS-induced RAW 264.7 macrophage cells. Planta Med. 2010;76(3):251-7.
Won, J. H., Shin, J. S., Park, H. J., Jung, H. J., Koh, D. J., Jo, B. G., Lee, J. Y., Yun, K., & Lee, K. T. (2010). Anti-inflammatory effects of madecassic acid via the suppression of NF-kappaB pathway in LPS-induced RAW 264.7 macrophage cells. Planta Medica, 76(3), 251-7. https://doi.org/10.1055/s-0029-1186142
Won JH, et al. Anti-inflammatory Effects of Madecassic Acid Via the Suppression of NF-kappaB Pathway in LPS-induced RAW 264.7 Macrophage Cells. Planta Med. 2010;76(3):251-7. PubMed PMID: 19774506.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory effects of madecassic acid via the suppression of NF-kappaB pathway in LPS-induced RAW 264.7 macrophage cells. AU - Won,Jong-Heon, AU - Shin,Ji-Sun, AU - Park,Hee-Juhn, AU - Jung,Hyun-Ju, AU - Koh,Duck-Jae, AU - Jo,Baek-Geon, AU - Lee,Jin-Yong, AU - Yun,Kijoo, AU - Lee,Kyung-Tae, Y1 - 2009/09/11/ PY - 2009/9/24/entrez PY - 2009/9/24/pubmed PY - 2010/6/19/medline SP - 251 EP - 7 JF - Planta medica JO - Planta Med VL - 76 IS - 3 N2 - We have investigated the anti-inflammatory effects of madecassic acid and madecassoside isolated from Centella asiatica (Umbelliferae) on lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cells. Both madecassic acid and madecassoside inhibited the production of nitric oxide (NO), prostaglandin E(2) (PGE(2)), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1beta), and IL-6. However, madecassic acid more potently suppressed these inflammatory mediators than did madecassoside. Consistent with these observations, madecassic acid inhibited the LPS-induced expression of iNOS and COX-2 at the protein level and of iNOS, COX-2, TNF-alpha, IL-1beta, and IL-6 at the mRNA level in RAW 264.7 macrophage cells, as determined by Western blotting and RT-PCR, respectively. Furthermore, madecassic acid suppressed the LPS-induced activation of nuclear factor-kappaB (NF-kappaB), and this was associated with the abrogation of inhibitory kappa B-alpha (IkappaB-alpha) degradation and with the subsequent blocking of p65 protein translocation to the nucleus. These results suggest that the anti-inflammatory properties of madecassic acid are caused by iNOS, COX-2, TNF-alpha, IL-1beta, and IL-6 inhibition via the downregulation of NF-kappaB activation in RAW 264.7 macrophage cells. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/19774506/Anti_inflammatory_effects_of_madecassic_acid_via_the_suppression_of_NF_kappaB_pathway_in_LPS_induced_RAW_264_7_macrophage_cells_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0029-1186142 DB - PRIME DP - Unbound Medicine ER -