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Intraperiaqueductal gray glycine and D-serine exert dual effects on rostral ventromedial medulla ON- and OFF-cell activity and thermoceptive threshold in the rat.
J Neurophysiol. 2009 Dec; 102(6):3169-79.JN

Abstract

We have studied the involvement of the N-methyl-D-aspartate receptor (NMDAR) glycine site and the strychnine-sensitive glycine receptor (GlyR) in the ventrolateral periaqueductal gray (VL-PAG) on nociceptive behavior (tail flick) and pain-related changes on neuronal activity in the rostral ventromedial medulla (RVM). Glycine or D-serine increased the tail-flick latency, reduced OFF-cell pause, and delayed its onset and increased the time between the onset of the OFF-cell pause and the tail withdrawal. Conversely, they decreased the ongoing activity of the ON cell, the tail-flick-induced ON-cell firing, whereas they delayed the onset of increased tail-flick-induced ON-cell firing. Also, glycine or D-serine reduced the interval between the onset of the increased ON-cell firing and tail withdrawal. Whereas 7-Cl-kynurenic acid (7-Cl-KYN) prevented such effects, strychnine did not do so. A higher dose of 7-Cl-KYN or strychnine was per se able to reduce or increase tail-flick latency and increase or reduce ON-cell activities, respectively. A higher dose of glycine was hyperalgesic in the presence of 7-Cl-KYN, whereas such an effect was prevented by strychnine. These data suggest 1) a dual role of glycine in producing hyperalgesia or analgesia by stimulating the GlyR or the NMDARs within the VL-PAG, respectively; 2) consistently that RVM ON and OFF cells display opposite firing patterns to the stimulation of the VL-PAG NMDAR glycine site and GlyR activation; and 3) a tonic role of these receptors within the VL-PAG-RVM antinociceptive descending pathway.

Authors+Show Affiliations

Department of Experimental Medicine, Second University of Naples, Naples, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19776366

Citation

Palazzo, Enza, et al. "Intraperiaqueductal Gray Glycine and D-serine Exert Dual Effects On Rostral Ventromedial Medulla ON- and OFF-cell Activity and Thermoceptive Threshold in the Rat." Journal of Neurophysiology, vol. 102, no. 6, 2009, pp. 3169-79.
Palazzo E, Guida F, Migliozzi A, et al. Intraperiaqueductal gray glycine and D-serine exert dual effects on rostral ventromedial medulla ON- and OFF-cell activity and thermoceptive threshold in the rat. J Neurophysiol. 2009;102(6):3169-79.
Palazzo, E., Guida, F., Migliozzi, A., Gatta, L., Marabese, I., Luongo, L., Rossi, C., de Novellis, V., Fernández-Sánchez, E., Soukupova, M., Zafra, F., & Maione, S. (2009). Intraperiaqueductal gray glycine and D-serine exert dual effects on rostral ventromedial medulla ON- and OFF-cell activity and thermoceptive threshold in the rat. Journal of Neurophysiology, 102(6), 3169-79. https://doi.org/10.1152/jn.00124.2009
Palazzo E, et al. Intraperiaqueductal Gray Glycine and D-serine Exert Dual Effects On Rostral Ventromedial Medulla ON- and OFF-cell Activity and Thermoceptive Threshold in the Rat. J Neurophysiol. 2009;102(6):3169-79. PubMed PMID: 19776366.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraperiaqueductal gray glycine and D-serine exert dual effects on rostral ventromedial medulla ON- and OFF-cell activity and thermoceptive threshold in the rat. AU - Palazzo,Enza, AU - Guida,Francesca, AU - Migliozzi,Annalucia, AU - Gatta,Luisa, AU - Marabese,Ida, AU - Luongo,Livio, AU - Rossi,Claudia, AU - de Novellis,Vito, AU - Fernández-Sánchez,Enrique, AU - Soukupova,Marie, AU - Zafra,Francisco, AU - Maione,Sabatino, Y1 - 2009/09/23/ PY - 2009/9/25/entrez PY - 2009/9/25/pubmed PY - 2010/2/23/medline SP - 3169 EP - 79 JF - Journal of neurophysiology JO - J Neurophysiol VL - 102 IS - 6 N2 - We have studied the involvement of the N-methyl-D-aspartate receptor (NMDAR) glycine site and the strychnine-sensitive glycine receptor (GlyR) in the ventrolateral periaqueductal gray (VL-PAG) on nociceptive behavior (tail flick) and pain-related changes on neuronal activity in the rostral ventromedial medulla (RVM). Glycine or D-serine increased the tail-flick latency, reduced OFF-cell pause, and delayed its onset and increased the time between the onset of the OFF-cell pause and the tail withdrawal. Conversely, they decreased the ongoing activity of the ON cell, the tail-flick-induced ON-cell firing, whereas they delayed the onset of increased tail-flick-induced ON-cell firing. Also, glycine or D-serine reduced the interval between the onset of the increased ON-cell firing and tail withdrawal. Whereas 7-Cl-kynurenic acid (7-Cl-KYN) prevented such effects, strychnine did not do so. A higher dose of 7-Cl-KYN or strychnine was per se able to reduce or increase tail-flick latency and increase or reduce ON-cell activities, respectively. A higher dose of glycine was hyperalgesic in the presence of 7-Cl-KYN, whereas such an effect was prevented by strychnine. These data suggest 1) a dual role of glycine in producing hyperalgesia or analgesia by stimulating the GlyR or the NMDARs within the VL-PAG, respectively; 2) consistently that RVM ON and OFF cells display opposite firing patterns to the stimulation of the VL-PAG NMDAR glycine site and GlyR activation; and 3) a tonic role of these receptors within the VL-PAG-RVM antinociceptive descending pathway. SN - 1522-1598 UR - https://www.unboundmedicine.com/medline/citation/19776366/Intraperiaqueductal_gray_glycine_and_D_serine_exert_dual_effects_on_rostral_ventromedial_medulla_ON__and_OFF_cell_activity_and_thermoceptive_threshold_in_the_rat_ L2 - https://journals.physiology.org/doi/10.1152/jn.00124.2009?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -