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Response to different oxidants of Saccharomyces cerevisiae ure2Delta mutant.
Arch Microbiol. 2009 Nov; 191(11):837-45.AM

Abstract

Growth of Saccharomyces cerevisiae ure2Delta mutant strain was investigated in the presence of diverse oxidant compounds. The inability of the strain to grow on a medium supplemented with H(2)O(2) was confirmed and a relationship between diminishing levels of glutathione (GSH) and peroxide sensitivity was established. Data for the lack of significant effect of URE2 disruption on the cellular growth in the presence of paraquat and menadione were obtained. The possible role of Ure2p in acquiring sensitivity to oxidative stress by means of its regulatory role in the GATA signal transduction pathway was discussed. It was suggested that the susceptibility of ure2Delta mutant to the exogenous hydrogen peroxide can result from increased GSH degradation due to the deregulated localization of the gamma-glutamyl transpeptidase activating factors Gln3/Gat1. The important role of Ure2p in in vivo glutathione-mediated reactive oxygen species (ROS) scavenging was shown by measuring the activity of antioxidant enzymes glutathione peroxidase, superoxide dismutase (SOD) and catalase in an URE2 disrupted strain. A time-dependent increase in SOD and catalase activity was observed. More importantly, it was shown that the ure2 mutation could cause significant disturbance in cellular oxidant balance and increased ROS level.

Authors+Show Affiliations

University of Strasbourg UMR 7156 CNRS, 28 rue Goethe, 67083 Strasbourg Cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19777209

Citation

Todorova, Tatina T., et al. "Response to Different Oxidants of Saccharomyces Cerevisiae ure2Delta Mutant." Archives of Microbiology, vol. 191, no. 11, 2009, pp. 837-45.
Todorova TT, Petrova VY, Vuilleumier S, et al. Response to different oxidants of Saccharomyces cerevisiae ure2Delta mutant. Arch Microbiol. 2009;191(11):837-45.
Todorova, T. T., Petrova, V. Y., Vuilleumier, S., & Kujumdzieva, A. V. (2009). Response to different oxidants of Saccharomyces cerevisiae ure2Delta mutant. Archives of Microbiology, 191(11), 837-45. https://doi.org/10.1007/s00203-009-0512-9
Todorova TT, et al. Response to Different Oxidants of Saccharomyces Cerevisiae ure2Delta Mutant. Arch Microbiol. 2009;191(11):837-45. PubMed PMID: 19777209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Response to different oxidants of Saccharomyces cerevisiae ure2Delta mutant. AU - Todorova,Tatina T, AU - Petrova,Ventsislava Y, AU - Vuilleumier,Stéphane, AU - Kujumdzieva,Anna V, Y1 - 2009/09/24/ PY - 2009/03/20/received PY - 2009/09/07/accepted PY - 2009/07/09/revised PY - 2009/9/25/entrez PY - 2009/9/25/pubmed PY - 2010/3/20/medline SP - 837 EP - 45 JF - Archives of microbiology JO - Arch Microbiol VL - 191 IS - 11 N2 - Growth of Saccharomyces cerevisiae ure2Delta mutant strain was investigated in the presence of diverse oxidant compounds. The inability of the strain to grow on a medium supplemented with H(2)O(2) was confirmed and a relationship between diminishing levels of glutathione (GSH) and peroxide sensitivity was established. Data for the lack of significant effect of URE2 disruption on the cellular growth in the presence of paraquat and menadione were obtained. The possible role of Ure2p in acquiring sensitivity to oxidative stress by means of its regulatory role in the GATA signal transduction pathway was discussed. It was suggested that the susceptibility of ure2Delta mutant to the exogenous hydrogen peroxide can result from increased GSH degradation due to the deregulated localization of the gamma-glutamyl transpeptidase activating factors Gln3/Gat1. The important role of Ure2p in in vivo glutathione-mediated reactive oxygen species (ROS) scavenging was shown by measuring the activity of antioxidant enzymes glutathione peroxidase, superoxide dismutase (SOD) and catalase in an URE2 disrupted strain. A time-dependent increase in SOD and catalase activity was observed. More importantly, it was shown that the ure2 mutation could cause significant disturbance in cellular oxidant balance and increased ROS level. SN - 1432-072X UR - https://www.unboundmedicine.com/medline/citation/19777209/Response_to_different_oxidants_of_Saccharomyces_cerevisiae_ure2Delta_mutant_ L2 - https://dx.doi.org/10.1007/s00203-009-0512-9 DB - PRIME DP - Unbound Medicine ER -